enteric bacteria 1 Flashcards

(58 cards)

1
Q

shigella stain and morphology

A

gram negative rod

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2
Q

is shigella lactose fermenting

A

no

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3
Q

is shigella sulfur producing

A

no

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4
Q

is shigella motile

A

no

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5
Q

what kind of metabolism does shigella have

A

facultative

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6
Q

is shigella intracellular or extracellular pathogen

A

intracellular

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7
Q

what is macConkey media

A

it is a selective differential media that selects for gram negative and differentiates between lactose fermenters

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8
Q

what is the infectious dose for shigella

A

very low

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9
Q

can shigella cause bacteremia

A

very rarely in malnourished children

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10
Q

what does shigella cause

A

fecal-oral gastroenteritis

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11
Q

what are the virulence factors for shigella

A

they are plasmid acquired. shiga toxin

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12
Q

what are the complications of shigella infection

A

reiters syndrome (reactive arthritis) and HUS

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13
Q

how does shigella infect>

A

enters through the M cells in peyers patches of the intestine. it exists the other side and is engulfed by macrophages. it causes the macrophage to apoptose, survives and enters the backside of the enterocyte via type three secretion system. it can infect neighboring cells

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14
Q

how do we diagnose shigella

A

on physical fever, dehydration, severe HA, progression from watery diarrhea.

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15
Q

what are the labs for shigella

A

agglutination, methylene blue stain for fecal matter.

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16
Q

what to look for when trying to diagnose HUS

A

look for schistocytes on blood smear, decreased platelets, increased molymorphonuclear leukocytes, increased LDH.

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17
Q

treatment for shigella

A

rehydration, ceftriaxone, fluoroquinolone, azithromycin, cefixime.

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18
Q

treatment for HUS

A

dialysis, supportive care.

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19
Q

prevention of shigella

A

hygiene, water treatment, handwashing

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20
Q

E. coli gram stain and mrophology

A

gram negative and rod

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21
Q

E. coli metabolism

A

facultative,

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22
Q

E. coli lactose fermenting

A

yes

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23
Q

E. coli sulfur

A

no

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24
Q

is E. coli motile

A

can be motile or nonmotile

25
where do we find E. coli
normal gut flora
26
what are the virulence factors for E. coli
they are plasmid acquired.
27
what is the pathogenesis of E. coli
gastroenteritis, bloody gastroenteritis, HUS, UTIs, meningitis, pneumonia
28
how does E. coli cause gastroenteritis
bacteria attach via fimbrae to the gut lining, endotoxins force the host gut to release fluids and potassium, there is watery diarrhea.
29
how does E. coli casue bloody diarrhea
adheres to gut lining and causes tissue destruction typically by strains carrying the shiga toxin plasmid. including O157:H7. there is also a risk for HUS in pediatrics.
30
who gets E. coli meningitis
neonates due to cranial trauma or surgery. there is a high mortality 8%. high morbidity
31
how do we get E. coli pneumonia
rare. typically disseminated from the GI or UTI
32
what is the percentage of E. coli HUS complete recovery
60%, 30% have major sequelae and 10% have DIC
33
E. coli diagnosis
physical. culture. check for HUS (schistocytes, decreased platelets, increased PMN, increased LDH.
34
how do grow E. coli
blood agar and differential medium.
35
treatment for E. coli gastroenteritis
rehydrate
36
treatment for bloody E. coli gastroenteritis
rehydrate (antibiotics are controversial)
37
treatment for HUS E. coli
rehydrate, dialysis, supportive care, NO ANTIBIOTICS
38
treatment for E. coli simple UTI
sulfa-trimethylprim or ampicillin.
39
treatment for E. coli simple UTI complicated by extension or sepsis
cephalosporin (cefotaxime)
40
treatment for E. coli meningitis
ampicillin and cefotaxime
41
prevention of E. coli
hand-washing, hygienic food delivery chain and home preparation. cranberry juice for UTI. nosocomial: remove catheters, switch IV often
42
where do we usually get S. aureus food borne gastroenteritis from and what is the pathogenesis?
protein food group. aureus secretes the toxin into room temperature food and it is later ingested.
43
bacillus cereus food borne illness?
causes the same symptoms and by the same mechanism as aureus. associated with rice, but can grow in room temperature foods.
44
what is the onset of aureus and cereus food borne illness?
fast. intox takes only hours. much faster than an infectious process.
45
clostridium botulinum intoxication?
this is a toxin, not a growth. gastroenteritis and descending flaccid paralysis caused by neurotoxin secreted into airtight foods
46
what is the onset for C botulinum poisoning
it is fast, but not as fast as staph
47
what does shiga toxin do
inhibits protein synthesis
48
what are shET1 and shET2
additional toxins for shigella
49
IcsA virulence
shigella. actin-based motility
50
shu and aerobactin?
shigella virulence factors for iro binding.
51
IpaB?
shigella virulence for macropohage apoptosis
52
what virulence factors does EIEC (e coli) have?
same as shigella
53
what virulence factor must the uropathogenic strain of E. coli have
P fimbrae
54
salmonella diseases?
enterocolitis, enteric fevers, septicemia, reactive arthritis
55
what virulence factors does salmonella have
capsule, adherence factors, toxins.
56
is there a vaccine to shigella?
no
57
is there a vaccine to E. coli
no
58
is there a vaccine to salmonella
yes, not commonly used