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Vet. Bacteriology > Enterobacteriaceae > Flashcards

Enterobacteriaceae Flashcards

1
Q

What kind of taxonomic unit is Enterobacteriaceae

A

Family

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2
Q

What other subunits are in Enterobacteriaceae

A

ca. 40 genera

ca. 180 species

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3
Q

where to find Enterobacteriaceae Bb

A

normal gut flora

through faeces into the environment

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4
Q

Most of them are pathogenic T/F

A

F most are saprophytes - can be helpful

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5
Q

Morphology

A

1-3 micrometer rod

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6
Q

All of them have flagella T/F

A

F all of them except shigella and klebsiella

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7
Q

Klebsiella and Shigella

A

have no flagella

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8
Q

Resistance

A

average

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9
Q

Staining

A

Gr -

red pinkish

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10
Q

Culture

A

simple aerobic, fac. anaerobic
10-45°c
1 day to produce colony

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11
Q

What is used for differentiation mainly

A

lactose fermentation

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12
Q

name a selective enrichment for Salmonella

A

Rambach agar

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13
Q

What do the differential media contain

A

lactose, indicator and inhibitory materials

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14
Q

What’s in a low selective capacity medium and what can grow in it

A

all kind of enterobacteriaceae and some Gr+ can grow
crysatl violet - lactose - litmus
crystal violet - bile salts - lactose - neutral red

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15
Q

What’s in a medium selective capacity medium and what can grow in it

A

only the most important enterobacteriaceae can grow

brilliant green - lactose - phenol red - E. and S.

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16
Q

High selective cap.

A

designed for isolation of salmonella

-bismuth sulphite agar

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17
Q

O/C

A

catalase +

oxidase -

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18
Q

O/F test

A

fermentative (acid + gas prod)

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19
Q

What else can be used for differentiation

A

the antigens

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20
Q

What are the antigens for differentiation

A

O, K, H, F

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21
Q

What do the antigens stand for

A 
O = cell wall
K= capsule
H= flagella
F= fimbira
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22
Q

Name the lactose pos. genera

A
  • Escherichia
  • Klebsiella
  • Enterobacter
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23
Q

Name the lactose neg. genera

A
  • Salmonella
  • Shigella
  • Yersinia
  • Proteus
  • Providencia
  • Edwardsiella
  • Serratia
  • Citrobacter
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24
Q

Most of Enterobacteriaceae are fac path T/F

A

F overwhelming majority is saprophytic

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25
Where are KLebsiella found
gut, resp. tract, Genital tract, environment, muc mem
26
Morphology of klebsiella
rod, capsullated, non flagellated
27
Describe the Klebsiella colonies
large merging colonies extensive capsule | fast metabolism
28
Enterobacteriaceae diff.
lactose + Voges proskauer + urease +
29
Name the relevant species of Klebsiella
K.pneumoniae
30
List the diseases a fac. K. pneumoniae could cause
Calf: bronchitis, bronchopneumonia Cow: mastits Mare: metritis Ho: pneumonia, urogenital infections
31
Where to find Enterobacter
gut, environment
32
Morphology of enterobacter
rod, thiccccccc capsule
33
Enterobacteriaceae diff
lactose + | Voges proskauer test+
34
Enterobacter is largely saprophytic T/F
T
35
What are the G- anaerobic rods
Fusobacterium, Bacteroides and Dichelobacter
36
Where to find the G- anaerobic rids
muc mem, alimentary tract, resp. tract, genital tract
37
Morph of Fusobacterium
polymorph
38
Culture of Fusobacterium
fastidious, obligate anaerobic, CO2
39
Species of Fusobacterium
F.necrophorum -ssp necroforum -ssp funduliforme F.nucleatum
40
What is the morphology of F.necrophorum
long chains, filaments
41
Culture of F.necrophorum
blood agar, obl. anaerobic, haemolysis
42
What diseases does F. necrophorum cause
ssp. necroforum causes Necrobacillosis in calf, piglet, rabbit and foot rot in sheep ssp funduliforme is saprophytic
43
What diseases does F.nucleatum cause
human carnivore abscess, mastitis, metritis
44
Morphology of bacteroides
3-5 micrometer rod with fimbria
45
Culture of bacteroides
fastidious , obl anaerobic
46
What's the most important spec. in Bacteroides
B.nodosus
47
What does B. nodosus cause
foot rot in sheep
48
What does Dichelobacter nodosus cause
foot rot in sheep
49
Where to find escherichia coli
gut, muc mem
50
Morphology of E.coli
2-3 mircom rod with capsule thiccc
51
Is E.coli flagellated
Yes
52
E-coli ha fimbria
Oh yeah
53
Culture of E.coli
aerobic, fac. anaerobic simple nutrient agar or broth colonies appear very fast
54
Enterobacteriaceae diff
lactose + | indol + (indol prod. from tryptophane)
55
list all the antigens of E.coli
O: type specific (174 types) K: heat stabile (A) and heat labile (L) : 80 types F: 19 types H:57 types
56
Which F antigens belong to which species
``` F1: common fimbria F2,F3: human F4,F6,F18:Su F5,F17,F41: Ru F7-16: UTI Ho and poultry ```
57
What can E.coli cause in Cattle
calf coli diarrhoea and septicaemia, cow mastitis metritis
58
What can E.coli cause in Su
neonatal pigs: coli-diarrhoea weaned piglets: coli-diarrhoea, edema disease Sow: mastitis, metritis
59
What can E.coli cause in lamb
Coli-diarrhoea
60
What can E.coli cause in poultry
dayoldchick: septicaemia chicken: septicaemia older: lesions in organs
61
What can E.coli cause in Ho
neonatal enteritis, septicaemia, urogenital infections, enteritis, haemorrhagic enterocolitis
62
!What protein do pathogenic strains of E.coli produce!
Intimin - attaches to surface of enterocytes and destroys them Enterotoxin - makes enterocytes excrete causing diarrhoea Necrotoxic - attack epithelial gut cells

Vet. Bacteriology (12 decks)

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