Staphylococcus Flashcards

1
Q

Where can Staphylococci be found

A

occur worldwide, commensals on skin, muc. memb.

food, plants, soil, water

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2
Q

Satphyl. can also sometime be found in the upper resp. tract and the lower Ug tract T/F

A

T

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3
Q

Morphology of S

A

Coccus 1 micrometer in diameter

form irregular clusters - bunch of grapes

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4
Q

S are flagellated Bb T/F

A

F

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5
Q

S are spore forming Bb T/F

A

F

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6
Q

S are non capsulated Bb T/F

A

T

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7
Q

S have flagella and a capsule while exposed to CO2 T/f

A

F

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8
Q

S are motile Bb despite having no flagella T/F

A

F

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9
Q

Which agar do S need

A

simple nutrient agar

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10
Q

Do S need oxygen

A

not necessarily

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11
Q

What are S in regard to oxygen

A

facultative anaerobs

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12
Q

How can S be categorized

A

coagulase +

coagulase -

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13
Q

On which medium can S be cultured

A

simple nutrient agar

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14
Q

Do S from pigments

A

yes

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15
Q

Which pigments do S form

A

white , golden (Ru,Ho strains)

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16
Q

How can you culture S selectively

A

10%NaCl - mannitol - phenolred agar
OR
Na tellurite - glycine - pyruvic acid - eggyolk - Baird parker agar

17
Q

What do S colonies look like on Baird parker agar

18
Q

C O

A

catalase +

oxidase -

19
Q

O F test

A

fermentative

20
Q

Characterise coagulase + S

A
  • ec enzymes
  • toxins
  • haemolysis
  • mannitol +
  • fac. path
21
Q

Characterizecoagulase - S

A
  • less ec enzymes
  • usually no toxin prod
  • no haemolysis
  • mannitol -
  • generally saprophytes
22
Q

Name the Coag. + Species

A
  • S.aureus ssp. aureus
  • S.aureus ssp. anaerobicus
  • S.pseudointermedius
23
Q

Name the Coag.- Species

A
  • S.hyicus
  • S.epidermidis
  • S.haemolyticus
  • S.gallinarum
  • S.equorum
  • S.Felis
24
Q

What does S.aureus ssp aureus cause

A

lucal suppuration, abscesses, infections after surgery

25
What does S.aureus ssp aureus cause in what species
``` Poultry: arthritis Ov,Ru,Goat,Su,Eq: mastitis Su: Metritis Ov, goat, Rabbit, poultry: dermatitis Ru,Su: udder impetigo Young lambs, rabbits turkey: septicaemia ```
26
What causes MOREL disease or caseous lymphadenitis of sheep
S.aureus ssp. anaerobicus
27
Pyoderma, pyometra, mastitis, endometritis of Dogs and cats is caused by which Bb
S.pseudointermedius
28
What does S.hyicus cause
exudative epidermitis (greasy pig disease) under 3 months of age
29
What do the rest of the Coagulase - S cause
dermatitis, local suppuration
30
How to perform Gram staining
- dry sample - crystal violet 3-5 min - wash - lugol 1-1,5 min - wash - 96% alc some drops - water immediately - fuchsin 0,5-1 min - wash - dry - enjoy