Enzyme: Kinetics Flashcards
(48 cards)
It lists the initial chemical species present and the new chemical species formed
Balanced chemical equation
It is used to describe reactions in living cells where the product of reaction are immediately consumed by subsequent enzyme catalyzed reaction
Unidirectional arrows
Describes both the direction in which a chemical reaction will tend to proceed and the concentration of reactant and products that will be present in equilibrium
Gibbs free energy change
_____ of the free energy change determines how far the reaction will proceed
Sign and Magnitude
G is independent of the _____ of the reaction and therefore provide no information concerning ____
Mechanism , rate
The natural and favorable tendency of process is to proceed from an area of
High energy to an area of low energy
Used to predict the direction of a reaction at a constant temperature Nd pressure
Standard free energy
Net loss of energy
Exergonic reaction
Net gain of energy
Endergonic reaction
Reaction in equilibrium
Equal
Spontaneous reaction
Exergonic reaction
Non spontaneous reaction
Enedergonic reaction
Forward reaction is equal to backward reaction
Reaction in equilibrium
Field of biochemistry concerned with the quantitative measurement of the rates of enzyme catalyzed reaction
Enzyme kinetics
Constant reaction and product
1 molar
Constant temperature
25C or 298 K
Constant temperature
1 atmosphere
It states that Standard free energy of all consecutive reactions in a pathway are additive
Coupling reaction or hess law
For 2 molecules to react, they must
Approach a bond forming distance
Possess enough kinetic energy to surpass energy barrier
Factors affecting collision
Substrate concentration
Temperature
pH
Inhibitors
It relates reaction rate to substrate concentration
Michaelis menten equation
Michaelis menten equation have _____ curve
Hyperbolic curve
Tell you how fast the reaction is
Vi or velocity
Michaelis constant is equal to
Half the maximal velocity
