Enzyme Kinetics Flashcards

(37 cards)

1
Q

What are the two types of irreversible inhibitions

A

Covalent Bonding

Suicide inhibitors

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2
Q

What are the three types of reversible inhibitions

A

Competitive, Uncompetitive, Non-Competitive

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3
Q

True or False, Km is decreased in the presence of a competitive inhibitor

A

False, it’s increases

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4
Q

True or False, maximal velocity is decreased in the presence of a competitive inhibitor

A

False it is constant

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5
Q

True or False, Non-competitive inhibition occurs when the inhibitor and the substrate binds to the same site on the enzyme

A

False, they bind on different sites

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6
Q

In non-competitive inhibition, Vmax is _______, do to the effects not being overcome by an increase in substrate concentration

A

Decreased

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7
Q

In non-competitive inhibition, Km is _________, due to the the inhibition not affecting the binding of the enzyme-substrate

A

Constant

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8
Q

Where does the inhibitor bind to in uncompetitive inhibition

A

Enzyme-substrate complex

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9
Q

Km and Vm __________ in uncompetitive inhibiton

A

Decreases

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10
Q

True or False, in non-competing inhibition, Inhibitor binds only to the enzyme

A

False, it binds to both enzyme and enzyme-substrate complex

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11
Q

In non-competitive inhibition, Vm ______ while Km ______

A

Decreased, remains the same

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12
Q

Allosteric Enzymes are regulated by ________

A

Effectors

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13
Q

What the names of the two binding sites for allosteric enzymes

A

Active and Allosteric

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14
Q

________ concentration affects allosteric enzymes

A

Substrate

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15
Q

Hb is an example of what type of non-michaelis menten kinetics

A

Cooperativity

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16
Q

What are the two states in a concerted model

A

Tense or relaxed

17
Q

When a ligand binds, it drives the equilibrium towards what state in a concerted model

18
Q

R concentration ________ apparent Km

19
Q

Inhibitor binds drives equilibrium to _______

20
Q

T concentration increases _______

21
Q

What disease causes a 3-6 month baby to not be able to turn over, sit or crawl

A

Tay-Sachs Disease

22
Q

What are the symptoms of Tay-Sachs disease

A

Seizures hearing loss, immobility

23
Q

What enzyme is absent in Tay-Sachs disease

A

Beta-Hexosaminidase A

24
Q

What is accumulated in Tay Sachs due to the absence of an enzyme

A

GM2 ganglioside

25
True or False, there is no cure for Tay-Sachs disease
True
26
What disease cause symptoms of intellectual disability, seizures and mental disorders
Phenylketonuria
27
What enzyme is absent to cause Phenylketonuria
Phenylalanine Hydroxylase
28
What disease is caused by a decrease of Phenylalanine
Phenylketonuria
29
What disease causes symptoms of severe gout, renal problems, poor muscle control
Lesch-Nyhan Syndrome
30
What enzyme is inhibited in Lesch Nyhan Syndrome
Hypoxanthine-guanine phosphoribosyltransferaee
31
What pathway is affected in Lesch Nyhan syndrome
Purine Salvage Pathway
32
What substance is built up in Lesch Nyhan syndrome
Uric Acid
33
True or False, there is no cure for Lesch-Nyhan Syndrome
True
34
What disease causes symptoms of Developmental Delay, Megaloblastic Anaemia, FTT
Hereditary Orotic Aciduria
35
What enzyme is inhibited in Hereditary Orotic Aciduria
Unridine Monophosphate Synthase
36
What pathway is affected in Hereditiry Orotic Aciduria
Pyrimidine - Urea cycle
37
What can be used to treat Hereditary Orotic Aciduria
Uridine Monophosphate/Uridine Triacetate