Enzyme Kinetics Flashcards
(37 cards)
What are the two types of irreversible inhibitions
Covalent Bonding
Suicide inhibitors
What are the three types of reversible inhibitions
Competitive, Uncompetitive, Non-Competitive
True or False, Km is decreased in the presence of a competitive inhibitor
False, it’s increases
True or False, maximal velocity is decreased in the presence of a competitive inhibitor
False it is constant
True or False, Non-competitive inhibition occurs when the inhibitor and the substrate binds to the same site on the enzyme
False, they bind on different sites
In non-competitive inhibition, Vmax is _______, do to the effects not being overcome by an increase in substrate concentration
Decreased
In non-competitive inhibition, Km is _________, due to the the inhibition not affecting the binding of the enzyme-substrate
Constant
Where does the inhibitor bind to in uncompetitive inhibition
Enzyme-substrate complex
Km and Vm __________ in uncompetitive inhibiton
Decreases
True or False, in non-competing inhibition, Inhibitor binds only to the enzyme
False, it binds to both enzyme and enzyme-substrate complex
In non-competitive inhibition, Vm ______ while Km ______
Decreased, remains the same
Allosteric Enzymes are regulated by ________
Effectors
What the names of the two binding sites for allosteric enzymes
Active and Allosteric
________ concentration affects allosteric enzymes
Substrate
Hb is an example of what type of non-michaelis menten kinetics
Cooperativity
What are the two states in a concerted model
Tense or relaxed
When a ligand binds, it drives the equilibrium towards what state in a concerted model
Relax
R concentration ________ apparent Km
Decreased
Inhibitor binds drives equilibrium to _______
T
T concentration increases _______
Km
What disease causes a 3-6 month baby to not be able to turn over, sit or crawl
Tay-Sachs Disease
What are the symptoms of Tay-Sachs disease
Seizures hearing loss, immobility
What enzyme is absent in Tay-Sachs disease
Beta-Hexosaminidase A
What is accumulated in Tay Sachs due to the absence of an enzyme
GM2 ganglioside