Enzyme ph and temp Flashcards
(7 cards)
1
Q
How varied pH
A
pH buffer.
2
Q
How temp varied
A
Water bath.
3
Q
How measured enzyme activity
A
Determined the volume of foam per minute.
4
Q
Why pH change affects enzyme activity
A
Active site is altered.
5
Q
Process ( pH)
A
Put pH buffer in cylinder/ add washing up liquid/ blend celery and filter it then put in solution/ add hydrogen peroxide to small cylinder/ in water bath at 25 degrees/ put h202 in solution/ record volume after a minute/
6
Q
Process ( temp)
A
Put pH buffer in cylinder/ add washing up liquid/ blend celery and filter it then put in solution/ add hydrogen peroxide to small cylinder/ in water bath at 0 degrees/ put h202 in solution/ record volume after a minute/
7
Q
Products
A
Water and O2
