Enzyme's Required Practical

Question 1

What is the first step in the enzyme required practical method?

Answer 1

Place single drops of iodine solution in rows on the tile

Iodine solution is used to test for the presence of starch.

Question 2

How do you label the test tube in the enzyme practical?

Answer 2

Label a test tube with the pH to be tested

This step is crucial for identifying which pH conditions are being tested.

Question 3

How much amylase is placed in the test tube?

Answer 3

2cm3 of amylase

Amylase is the enzyme being tested for its activity on starch.

Question 4

What is added to the test tube along with amylase?

Answer 4

1cm3 of pH buffer solution

The buffer solution is important for maintaining a constant pH during the experiment.

Question 5

What should be done after adding starch solution to the amylase and buffer solution?

Answer 5

Start the stopwatch whilst mixing using a pipette

Timing is essential to measure the enzyme activity accurately.

Question 6

What indicates the presence of starch in the iodine test?

Answer 6

Blue-black color

The blue-black color occurs when iodine interacts with starch.

Question 7

What is the interval for adding drops of the mixture to the iodine solution?

Answer 7

Every 10 seconds

This consistent timing allows for measuring the rate of starch digestion.

Question 8

What does it mean if the iodine solution remains orange-brown?

Answer 8

All the starch has been digested

An orange-brown color indicates the absence of starch.

Question 9

What happens to the time taken for the iodine solution to change color at different pH values?

Answer 9

The less time, the quicker all the starch has been digested

This reflects how well the enzyme works at that specific pH.

Question 10

Fill in the blank: Use another test tube to add _______ of starch solution to the amylase and buffer solution.

Answer 10

2cm3

The volume of starch solution is standardized for consistency.