Enzymes 1 Flashcards
Two models of substrate binding
Lock and key
Induced fit
What does substrate binding induce
Conformational change (enhanced interaction). Transitional state complex is formed which is an unstable complex in which bonds in substrate are strained
What is the lock and key model
-binding through hydrophobic, es and hydrogen bonds
In the lock and key model, what is binding prevented by
steric hindrance and charge repulsion
In induced fit, what happens
Side chains of amino acids reposition
Binding interactions increase
Why are transitional state complexes useful in the pharmaceutical industry
Bind more tightly to enzymes than substrates so when making an inhibitor, can make a transition state comples
What are coenzymes
Non peptide molecules
What can we get coenzymes from
vitamins: water soluble or lipid soluble
Classes of coenzymes and their functions
Activation transfer
Ox-Red
What do activation transfer enzymes do and what bonds do they form
move functional groups around
form covalent bonds
What do ox-red enzymes do
Help electrons on/off
What do metal ions in catalysis do
electrophiles can stable anions
What are isoenzymes
Different amino acid sequence but catalyst same reaction
What are multi enzyme complexes
Enzymes that are similar/in same biological pathway stay together and associate to form a multi-enzyme comples
Different types of enzymes in serum
Serum specific enzymes
Secreted enzymes
Non serum specific enzymes (i.e. released due to cell turnover/damage)
