Enzymes

Question 1

The appearance of LD-1/LD-2 flipped pattern and the presence of CK-MB isoenzyme on electrophoresis is highly suggestive of?

Answer 1

Myocardial Infarction

Question 2

classification/sub-classification of Alanine amino transferase

Answer 2

Aminotransferase

Question 3

classification/sub-classification of creatine phosphokinase

Answer 3

Phosphotransferase

Question 4

classification/sub-classification alkaline phosphate

Answer 4

Esterase

Question 5

classification/sub-classification of amylase

Answer 5

Glycosidase

Question 6

classification/sub-classification of lactate dehydrogenase

Answer 6

Oxidoreductase

Question 7

Disease causing increase in ACP

Answer 7

Hairy cell leukemia

Question 8

Disease causing increased in CK-MB

Answer 8

Myocardial infarction

Question 9

Disease causing increased in amylase

Answer 9

Pancreatitis

Question 10

Disease causing increased in AST

Answer 10

Muscle trauma

Question 11

Disease causing increased in lipase

Answer 11

Pancreatitis

Question 12

In enzyme kinetics, what do we call the speed of enzyme reaction that initially increases as the substance increases?

Answer 12

First order kinetics

Question 13

Which of the following methods for alkaline phosphatase uses buffered phenolphthalein phosphate as substrate?

Answer 13

Klein-Babson-Reed

Question 14

One of the following is a coenzyme in the LDH catalyzed reaction

Answer 14

NAD/NADH

Question 15

The De Ritis ratio is a very useful indicator of the etiology of liver disease. What is the indication if the De Ritis ratio is very high?

Answer 15

Alcoholic liver disease

Question 16

The following laboratory results is suggestive of what condition?
I. Alkaline phosphatase = extremely high II. Direct bilirubin = normal
III. Gamma glutamyl transferase=normal IV. Alanine transferase= normal

Answer 16

Paget’s disease

Question 17

One of the following is not a reason for increased enzyme activity.

Answer 17

Reaction lacks co-factors

Question 18

Tissue source of ACP

Answer 18

Rbc

Question 19

Tissue source of lipase

Answer 19

Pancreas

Question 20

Tissue source of AST

Answer 20

Skeletal muscle

Question 21

Tissue source of amylase

Answer 21

Pancreas

Question 22

Tissue source of CK-MB

Answer 22

Myocardium

Question 23

Which of the following functions as a coenzyme in transaminase reaction?

Answer 23

Pyridoxal phosphate

Question 24

The term to describe loss of activity of the enzyme because of physical processes such as heat, mechanical agitation, irradiation is called?

Answer 24

Denaturation

Question 25

In amylase determination, the method that measures the disappearance of starch substrate is:

Answer 25

Amyloclastic

Question 26

Which of the following enzymes exhibit the least tissue specificity?

Answer 26

LDH

Question 27

What ALP isoenzyme has the same properties as the Regan isoenzyme?

Answer 27

Placental

Question 28

LDH levels are increased in the following disease except:

Answer 28

Pancreatitis

Question 29

catalyzes the removal of a group of atoms from the substrate

Answer 29

Lyases

Question 30

Catalyzes group transfer reaction

Answer 30

Transferase

Question 31

Catalyzes hydrolytic reaction

Answer 31

Hydrolase

Question 32

Catalyzes the reduction reactions

Answer 32

Oxido-reductase

Question 33

Catalyzes reactions involving isomeration

Answer 33

Isomerase

Question 34

This theory allows some degree of conformational change after binding of substrate and active site so as to form a stronger association.

Answer 34

Induce fit

Question 35

From the items listed below, identify the reason for the increase in urinary amylase activity in acute pancreatitis.

Answer 35

There is an increased renal clearance of amylase

Question 36

One of the following is the term used when an enzyme activity being measured is coupled with a colorimetric reaction.

Answer 36

Coupled enzyme reaction

Question 37

The enzyme that acts on several phosphate ester substrates and exhibit low tissue specificity is:

Answer 37

ALP

Question 38

One of the following is the term used when an enzyme activity being measured is coupled with a colorimetric reaction.

Answer 38

Coupled enzyme reaction

Question 39

The enzyme that acts on several phosphate ester substrates and exhibit low tissue specificity is:

Answer 39

ALP

Question 40

Which of the following is the substrate of choice for measuring acid phosphatase activity?

Answer 40

Thymolphthalein monophosphate

Question 41

Which amylase of the following methods for amylase determination measures the amount of starch hydrolyzed within a specified time?

Answer 41

Amylometric

Question 42

The end color of starch using iodine after its completely hydrolyzed.

Answer 42

Brown