Enzymes Flashcards
(12 cards)
The effect of pH on the activity of an enzyme
Decreases away from the optimum of pH
The change in pH changes the secondary/tertiary structure
Disruption of active sites prevents substrate from binding so fewer enzyme substrate complexes can form
Advantages of having a wide range of pH
It can work in a range of areas in and out of the body
State the term given to enzymes which work within specific compartments inside cells
Intracellular
Why would it be inappropriate to use a buffer solution on an experiment test in the effect of temperature on hydrolysis on fatty acids
Colour change is the dependent on a change in pH
Hydrolysis producers fatty acids that lower the pH
Buffer solution would stabilise pH
Why is a buffer solution necessary?
Maintains pH by keeping pH at optimum
Prevents changes in pH which would alter the rate of reaction
Why would you keep the temperature the same in an enzyme controlled experiment
To maintain temperature, so that all the enzymes and substrate have the same kinetic energy
How can biosensors be used to give a measurement of glucose concentration?
Equation shows a one-to-one relationship
The concentration of hydrogen peroxide is converted to an electrical signal detected by the electrode
How does an enzyme speed up the rate of reaction?
Lowers the activation energy needed for the reaction
What is the limiting factor in an enzyme graph and what happens to the active sites at higher concentrations
Enzyme concentration and number of active sites is the limiting factor
Active site on the enzyme are full
What happens to immobilised enzymes above 50 degrees
The immobilisation stabilises the enzyme maintaining the shape of its active site
Why would reducing the flow rate of a material through a column result in increased liquid being collected?
Increases contact time between enzyme and substrate so more successful collisions and more enzyme substrate complexes can form
Differences between enzymes bound to the gel membrane surface with those immobilised inside beads
Membrane bound enzymes are more efficient because they are directly exposed to substrate
Enzymes immobilised inside bead substrate has to diffuse into bead
