Enzymes

Question 1

Enzymes are…

Answer 1

Biological catalysts

Question 2

What do enzymes do

Answer 2

They speed up chemical reactions in living organisms

Question 3

Substrate?

Answer 3

Molecule that gets changed in a chemical reaction

Question 4

Active site

Answer 4

Part where the enzyme joins in to its substrate to catalyse reaction

Question 5

Enzymes are substrate…

Answer 5

Specific

Question 6

Substrate specific

Answer 6

Enzyme only works with only one substrate

Question 7

Enzymes catalyse breakdown and…

Answer 7

Synthesis reactions

Question 8

Enzymes called……..break down carbohydrates into simple sugars

Answer 8

Carbohydrases

Question 9

Name a type of carbohydrase

Answer 9

Amylase

Question 10

What does amylase do?

Answer 10

Break down starch

Question 11

Proteases break down protein into…..

Answer 11

Amino acids

Question 12

Other enzymes make protein…. amino acids

Answer 12

From

Question 13

Lipase breaks down lipids into…….

Answer 13

Glycerol and fatty acids

Question 14

Higher temperature….. rate of enzyme catalysed reaction

If it gets too hot. Then bonds holding enzyme together will…..

This changes the shape of the enzymes active site so the…….won’t fit

Answer 14

Increased

Break

Substrate

Question 15

What is it called if the substrate won’t fit in active site anymore?

Answer 15

Denatured

Question 16

What is the temperature enzymes work best at?

Answer 16

Optimum Temperature

Question 17

What is the neutral optimum ph

Answer 17

7 ph

Question 18

What ph does pepsin work best at

Answer 18

Ph 2

Question 19

Where is pepsin

Answer 19

In the stomach

Question 20

The higher the substrate concentration….

Answer 20

The faster the reaction

Question 21

Why is it that the higher the substrate concentration, the faster the reaction

Answer 21

Because it is more likely that the enzyme will meet up and react with substrate molecule

Question 22

Practical on investigation of how ph affects enzyme activity

Answer 22

1. Put a drop of iodine solutions into every well of spotting tile
2. Set up water bath at 35°C
3. Add amylase solution and buffer solution in pH 5 into boiling tube
4. Put boiling tub in the water bath and wait 5 mins
5. add starch solution to boiling tube, mix and start stop clock
6. Every 10 seconds take a sample from boiling tube using a dropping pipette
7. Put a drop of sample into well on spotting tile
8. When iodine solution stays browny-orange all starch in sample has been broken down
9. Repeat experiment with buffer solution of different pHs

Question 23

As the pH changes what else will change?

Answer 23

The time it takes for starch to be broken down

Question 24

What should stay the same to make it a fair test?

Answer 24

Concentration and volume of amylase solution

Question 25

Rate of reaction?

Answer 25

Rate = 1000 ÷ time