Enzymes and Catalysis (Chymotrypsin hydrolysis part)

Question 1

What family is chymotrypsin part of and what is it sequence specificity?

Answer 1

• Family: serine proteases; digestive enzyme in small intestine
• Sequence specificity: hydrolyses on the C terminal side of hydrophobic amino acids (Trp, Tyr, Phe)
  
  • A related enzyme is trypsin which hydrolysis before basic residues (Arg, Lys)

Question 2

State the function of the components of chymotrypsin (catalytic triad, amide backbone, hydrophobic pockets)

Answer 2

Question 3

Describe step 1 of chymotrypsin catalysis (of peptide hydrolysis)

Answer 3

Question 4

Describe step 2 of chymotrypsin catalysis (of peptide hydrolysis)

Answer 4

Question 5

Describe step 3 of chymotrypsin catalysis (of peptide hydrolysis)

Answer 5

Question 6

Describe step 4 of chymotrypsin catalysis (of peptide hydrolysis)

Answer 6

Question 7

Describe step 5 of chymotrypsin catalysis (of peptide hydrolysis)

Answer 7

Question 8

Describe step 6 of chymotrypsin catalysis (of peptide hydrolysis)

Answer 8