Enzymes used in Recombinant DNA------------------------------------------ Flashcards Preview

MABS Biochemistry > Enzymes used in Recombinant DNA------------------------------------------ > Flashcards

Flashcards in Enzymes used in Recombinant DNA------------------------------------------ Deck (12)
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1
Q

define Restriction Endonucleases

A

cut double stranded DNA at specific sequences. Used in cloning and some diagnostic assays (e.g., RFLP).

2
Q

define DNA polymerase

A

polymerizes a new DNA molecule that is complementary to an existing template strand. It has many uses in molecular biology.

3
Q

define heat stable DNA polymerases

A

a class of DNA polymerases, isolated from thermophilic bacteria, that remain active at high temperatures. Taq DNA polymerase (from Thermus aquaticus) is the most common example. Uses include PCR and cycle sequencing.

4
Q

define DNA ligases

A

forms a phosphodiester linkage between the 5’-phosphate and the 3’-hydroxyl of adjacent nucleosides on “nicked” DNA. Used in cloning.

5
Q

define RNA polymerases

A

polymerizes an RNA molecule that is complementary to an existing DNA template.

6
Q

define Reverse Transcriptase

A

polymerizes a DNA molecule that is complementary to an RNA template. It is used in the production of cDNA and to identify transcriptional start-sites of genes.

7
Q

polymerizes a DNA molecule that is complementary to an RNA template. It is used in the production of cDNA and to identify transcriptional start-sites of genes.

A

Reverse Transcriptase

8
Q

polymerizes an RNA molecule that is complementary to an existing DNA template.

A

RNA polymerases

9
Q

forms a phosphodiester linkage between the 5’-phosphate and the 3’-hydroxyl of adjacent nucleosides on “nicked” DNA. Used in cloning.

A

DNA ligases

10
Q

a class of DNA polymerases, isolated from thermophilic bacteria, that remain active at high temperatures. Taq DNA polymerase (from Thermus aquaticus) is the most common example. Uses include PCR and cycle sequencing.

A

heat stable DNA polymerases

11
Q

polymerizes a new DNA molecule that is complementary to an existing template strand. It has many uses in molecular biology.

A

DNA polymerase

12
Q

cut double stranded DNA at specific sequences. Used in cloning and some diagnostic assays (e.g., RFLP).

A

Restriction Endonucleases