ENZYMOLOGY

Question 1

also called relative specificity

Answer 1

Bond specificity

Question 2

Apoenzyme + Coenzyme

Answer 2

Holoenzyme

Question 3

Chemical reactants which bind with enzymes

Answer 3

Substrate

Question 4

Class of enzymes

Answer 4

Oxidoreductases
Transferases
Hydrolases
Lyases
Isomerases
Ligases

Question 5

Enzymes that catalyze the same chemical reaction but have slightly different structures

Answer 5

Isoenzyme

Question 6

Examples of lyases

Answer 6

Glutamate decarboxylase
Pyruvate decarboxylase
Tryptophan decarboxylases
Aldolase

Question 7

Factors that affect Km

Answer 7

pH
Temperature
Ionic strength
Nature of substrate

Question 8

In the nomenclature of enzymes, the 4th digit signifies what

Answer 8

Serial number

Question 9

In what temperature can enzyme denaturation occr

Answer 9

40-50C

Question 10

Key part of enzyme structure

Answer 10

Active site

Question 11

LDH is a type of

Answer 11

Isoenzyme; Oxidoreductase

Question 12

Methods used to measure enzyme reactions

Answer 12

Fixed time or end point analysis

Continuous monitoring/ kinetic assay

Question 13

Place for regulator binds

Answer 13

Allosteric site

Question 14

Serve as second substrate for enzymatic reactions

Answer 14

Coenzyme

Question 15

T or F: all enzymes contain allosteric site

Answer 15

F

Question 16

T or F: enzymes are not high molecular weight compounds

Answer 16

F

Question 17

T or F: reaction rate is directly proportional to the concentration of the substrate

Answer 17

T

Question 18

The enzyme nomenclature is made by

Answer 18

Enzyme commission of International Union of Biochemistry

Question 19

Theory in which the interaction of the enzyme and substrate causes a mild shift in the structure of the enzyme that confirms an ideal binding arrangement between the two

Answer 19

Induced Fit Theory

Question 20

Types of Specificity

Answer 20

Absolute specificity
Group specificity
Bond specificity
Sterioisometric specificity

Question 21

Most physiologic reactions occur in the pH __

Answer 21

7-8

Question 22

Amount of enzyme that catalyzes 1 micromole of substrate/minute

Answer 22

International unit (IU)

Question 23

Amount of enzyme w/c converts 1 mole of substrate per second

Answer 23

Katal

Question 24

Catalyze the removal or addition of electrons

Answer 24

Oxidoreductases

Question 25

Difference of competitive inhibitor and noncompetitive inhibitor

Answer 25

Competitive inhibitor competes for the active site, while the non competitive inhibitor binds to the allosteric site causing the change in shape of the enzyme

Question 26

Factors affecting enzyme reactions

Answer 26

``` Substrate concentration Enzyme concentration pH Temperature Cofactors Inhibitors ```

Question 27

Kinds of Inhibitors

Answer 27

Competitive Noncompetitive Uncompetitive

Question 28

Substances which decrease the rate of enzyme reaction if they are present in the reaction system

Answer 28

Inhibitors

Question 29

In what temperature is the analysis of enzymes carried out?

Answer 29

25C, 30C, 37C

Question 30

T or F Increasing the concentration of cofactor will decrease the rate of enzyme reaction

Answer 30

False

Question 31

Nomenclature of Amylase

Answer 31

EC 3.2.1.1

Question 32

Nomenclature of Alanine aminotransferase

Answer 32

EC 2.6.1.2

Question 33

Catalyze the hydrolysis of a bond by the addition of water

Answer 33

Hydrolases

Question 34

Catalyze intermollecular arrangement of the substrate

Answer 34

Isomerases

Question 35

Catalyze the joining of two molecules

Answer 35

Ligases