Epithelial Tissue Wk1

Question 1

What is the histology?

Answer 1

= Study of micro anatomy on a cellular level

• Understanding how cells are organised into specific functional tissues –> organs.
• Histopathology in diagnostics: cancer can be diagnosed
• Researching human disease, drug treatments and new biomarkers

Question 2

How do you study tissues?

Answer 2

1. Fix the fresh tissue e.g. with formaldehyde or freezing, and then thinly slice it
2. Prepare the tissue
3. Has to have wax/resin removed, and then dyed. The most commonly used dye is haemotoxylin and eosin (H&E). Most dyes have a chemistry of acid/base dyes e.g. basic dyes will bind to acidic parts of the cell (nucleus is acidic).
4. Visualise tissue under microscope

Question 3

What colour does haematoxylin stain?

Answer 3

Nucleus - purple

Question 4

What colour does eosin stain?

Answer 4

Nucleus - pink
Cytoplasm - Pink
Connective Tissue - Pink

Question 5

What colour does H&E stain?

Answer 5

Nucleus - purple
Cytoplasm - pink
Connective Tissue - pink

Question 6

What colour does haematoxylin and van Gieson stain?

Answer 6

Nucleus - Grey-blue
Cytoplasm - Green-yellow
Connective Tissue - (collagen) red

Question 7

What colour does Masson’s trichome stain?

Answer 7

Nucleus - purple
Cytoplasm - brick red
Connective Tissue - green or blue

Question 8

What colour does elastic van gieson stain?

Answer 8

Nucleus - Grey-blue
Cytoplasm - Green-yellow
Connective tissue - Red (collagen), Black (elastic fibres)

Question 9

What can immunochemistry do?

Answer 9

Assess the expression of particular proteins, depending on the antibody used

Question 10

What is a biopsy?

Answer 10

Taking a small sample of a growth. Cervical cancer biopsies are typically taken for histopathology.

Question 11

What is neoplasia?

Answer 11

Abnormal or excessive tissue growth

Question 12

What is metaplasia?

Answer 12

Transformation of one differentiated cell type to another

Question 13

What are the 4 classifications of tissue?

Answer 13

• Muscle
• Epithelium
• Connective tissue
• Neuronal tissue

Question 14

What is the basement membrane?

Answer 14

• Forms the barrier between the outside and inside
• Consists of several layers of extracellular matrix
• Structural support

Question 15

What is the nomenclature of epithelial tissue?

Answer 15

Number of layers:
1 = simple
1+ = stratified
Shape of cells:
Square&Circle = Cuboidal