ETC - online questions Flashcards
1 NADH = How many ATP
2.5 ATP
1 FADH2 = How many ATP
1.5 ATP
How many H+ ions does it take to make 1 ATP
4 H+
1 NADH pumps how many H+ across
10
1 FADH2 pumps how many H + across
6
TCA occurs where
Matrix of mitochondria
OP Occurs where
Inner membrane of mitochondria
At the last step of OP each NADH or FADH2 produces what?
One water molecule
1 Glucose molecule = how many ATP
32 ATP (experimental yield)
Explain how NADH goes through the electron transport chain
Complex I > UQ > Complex 3 > Cytochrome C > Complex 4
Name each complex (4 of them) in the electron transport chain and how many hydrogen’s are pumped across
Complex I - 4 H+
Which complex is water created by
Complex IV
What kind of gradient is created by the electron transport chain
A chemical and electrical gradient
ATP Synthase is made up of what
F0 and F1
F0 is
The porin part or the part that lets the H+ ions go through it
F1 is
The active site for the ADP > ATP reaction
What are the 3 active sites in ATP Synthase in order
L > T > O
Which is more negative ADP or ATP and why is this important?
ATP is more negative ; allows it to get out of the mitochondria due to the electrical gradients
How do NADH from glycolysis get into the mitochondria?
2 Different Shuttle Mechanisms
The Glycerophosphate Shuttle does what
NADH > FADH2
What enzyme is used?
Glycerophosphate Dehydrogenase
Malate Asparate Shuttle does what?
NADH > NADH
Explain what happens
Oxaloacetate > Aspartate > goes and gets electrons off NADH > goes back into mitochandria > Oxaloacetate
