Ethanol Gelation Test

Question 1

Ethanol gelation test is designed to _

Answer 1

detect the presence of fibrin monomers in the plasma

Question 2

It is a screening procedure to be utilized as an aid in the diagnosis of __ and in distinguishing this condition from __

Answer 2

Disseminated intravascular coagulation and distinguishing this condition from primary hemostasis

Question 3

What is the principle?

Answer 3

When ethanol and sodium hydroxide is added to plasma containing soluble fibrin monomers, precipitation or gel is formed

Question 4

Enumerate the materials needed

Answer 4

• test tubes 12x75 mm
• 50% ethyl alcohol
• 0.1 N sodium hydroxide
• pipettes
• 70% alcohol
• cotton
• 5ml syringe/vacutainer needle

Question 5

What is the specimen needed?

Answer 5

Fresh citrated plasma

Question 6

PROCEDURE: FILL IN THE BLANKS

1. ___ specimen to obtain the ___.
2. Add ___ of patient’s __ to a test tube.
3. Add ___ of ___ to the test tube with plasma and mix gently.
4. To the mixture, add ___ of ___ and place in a test tube rack at room temperature for ___.
5. Tilt the tube and check for ___ or ___. If precipitate or gel forms, add __ of ___ to the tube and mix.
6. Read and report result

Answer 6

1. Centrifuge specimen to obtain the plasma.
2. Add 9 drops of patient’s plasma of patient’s __ to a test tube.
3. Add 1 drops of 0.1N NaOH to the test tube with plasma and mix gently.
4. To the mixture, add 3 drops of 50% ethanol and place in a test tube rack at room temperature for 10 minutes.
5. Tilt the tube and check for precipitation or gel formation. If precipitate or gel forms, add 1 more drop of 0.1N NaOH to the tube and mix.
6. Read and report result

Question 7

Negative results indicate __

Answer 7

Absence of precipitate or gel formation

Question 8

positive result indicates __

Answer 8

Presence of precipitate or gel formation

Question 9

Normal result indicates ___

Answer 9

Negative

Question 10

Ratio of anticoagulant to blood

Answer 10

1:9

Question 11

Centrifugation speed and time

Answer 11

1,500 rpm for at least 10 mins

Question 12

Blood needed

Answer 12

Venous blood

Question 13

What is the action of ethanol in converting soluble fibrin monomer to insoluble fibrin

Answer 13

After ethanol and NaOH is added, the precipitation or gel will be formed.

Question 14

Explain the indirect significance of a positive result to the level of factor XIII in the patient’s plasma.

Answer 14

Presence of fibrin monomers could indirectly signify the levels of factor XIII since it is the one responsible for stable fibrin clot.