Exam 1 Flashcards

(62 cards)

1
Q

Lecture 1 - Bony Fish - Teleost

A
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2
Q

Anatomically unique characteristics

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Heart
-2 chambers: atrium and ventricle
-Gill archers: bony arcades, bilaterally symmetrical
-Operculum: musculoskeletal flap covering each set of gill rakers; aids in respiration
-Integumentary system: scales, protective mucus layer against parasites and bacteria

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3
Q

Nitrification process involving bacteria in a stable aquarium

Which bacteria converts ammonia to nitrites, and which nitrites to nitrates?

A

Aquarium nitrogen cycle

Waste products

-Ammonia: NH4
-Nitrites: NO2
-Nitrates: NO3

Nitrosomonas: ammonia to nitrites

Nitrospira/nitrobacter: nitrites to nitrates

Excess NO3 (nitrates) removed via partial water exchanges

Do not remove entire volume of water to avoid interrupting nitrogen bacterial cycle

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4
Q

Trace the blood flow through the teleosts heart and gill rakers

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5
Q

State and explain the principles of general anesthesia for most teleosts with aqueous solution of general anesthetic using tricaine methane sulfonate, MS 222

A

Tricaine Methane Sulfonate salt
a.k.a MS 222

-ml/L
-Concentration varies w/depth of anesthesia
-Add buffering agent bc water becomes acidic and it can scald the fish’s gills
-Drug absorbed through the gills into the blood stream
-Recover chamber a second water tank

  1. Aqueous solution prepared and tank prepared including buffer
  2. Introduce fish
  3. Separate and recovery in different tank

Diagnostic sampling with general anesthesia

Venipucture & Cytology
-Vertebral tail vein: dorsal, ventral, right lateral, or left lateral tail veins
-Gill arch: brachial vessel

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6
Q

Gill specimen

A

Biopsy: gill specimen

-Normal vs. abnormal architecture of gill filaments

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7
Q

What are 5 examples of fish diseases?

A
  1. Ich: Icthyophthirius multifiliis (White spot disease)
  2. Whirling disease: Myxobolus cerebral
  3. Anisakis spp: nematode
  4. Clonorchis sinuses: trematode
  5. Dyphyllobothrium datum: cestode
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8
Q

State and understand the etiologic agents and pathogenesis of specified zoonotic fish diseases

A
  1. Fungal: Ichthyophthirius multifiliis “Ich”

-Life stages: Theront = in water
Trophont = in the fish

Tx
-Formaldehyde @25ppm
-Malachite: copper toxicity risk for other species in the aquarium
-Elevating water temp for 5-7 days

  1. Myxobolus cerebral: Whirling disease

-Histozoic parasite that infects cultures and wild salmonids
-Specific tropism for cartilage
-Infection can result in axial skeletal and neural damage

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9
Q

Zoonosis risk of consuming raw fish

A
  1. Anisakis spp. Nematode (roundworm)
    -Intestinal migration, peritoneum infection 6-8hr post ingestion severely sick and life threatening
    -Eggs, Crustaceans, forage fish, predator fish, human.
  2. Clonorchis sinesis: trematode (chinese-fluke)
    -Fresh water fish
    -Incubation period 1-2 months
    -Dx: eggs in feces
    -Tx: praziquantel or Choramphenicol (aplastic anemia)
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10
Q

Zoonosis risk of consuming raw fish

A
  1. Diphylloothrium bothrum: cestode (tapeworm)
    -Carnivore or human host is infected by ingesting poorly cooked or raw fish containing the plerocercoids.
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11
Q
A
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12
Q

Lecture 2 Elasmobranchs - Cartilaginous fish

A

Sharks & Rays

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13
Q

Elasmobranchs Sub-groups

A

Galea & Squala: Sharks (350 spp.)

Batoids: Skates and Rays

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14
Q

Skates Vs. Rays

A

Skates

-Enlarged thorns along the midline of the back extending onto the tail
-Lay eggs (oviparous)
-Pelvic fins have two lobes (bilobate)
-Males have alar spines (wingtips) and malar spines (eye spines)

Rays

-No thorns (or buckles) along the midline of the back
-Tail has whip-like stinging spine midway, usually no dorsal fins
-Each pelvic fin has only one lobe
-Males do not have alar nor molar spines
-Give life birth (viviparous)

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15
Q
A
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16
Q

Galea & Squalea - Sharks

A

External anatomical features

-5-7 pairs of gill slits
-Gill slits open laterally
-Stiff, triangular dorsal fin
-Broad pectoral fins
-No bones, cartilaginous skeleton

Internal anatomy

-Intestinal tract posses SPIRAL VALVE to add surface area for nutrient absorption
-Coelomic cavity
-Liver extremely large compared to other viscera
-2 chambers heart
Route of the blood:
Sinus venous, Atrium, semi-lunar valve, ventricle, conus arteriosus, brachial arches by gills

Venipuncture
-Caudal vertebra vein
-Lateral or ventral approach

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17
Q

Shark respiratory system

A

-Ram water circulation: flow through oral cavity and exit via gills
-Gas exchange occurs at the gill filaments
-Previous theory that they have to locomote to breath, but pharyngeal region allows respiration at rest

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18
Q

Nicitating membrane - EYE

A

-Protects the eye globe during attacks

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19
Q

Sense of Hearing and Touch and Smell

A

Ear

-Vibration and sound detected by ACOUSTIC-LATERALIS system
-Inner ear structure 3 chambers and ear stone = OTOLITH
-They can detect sound, acceleration and gravity

Touch

-LATERAL LINE system
-Neuromats = sensory cells, hair-like structures project into clean and detect vibrations, turbulence and or currents

Smell

-Olfatory organs = blind sacs not connected to the mouth
-Smell blood cells

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20
Q

Sense of Electroreception

A

-Pores in the snout
-AMPULLAE of LORENZINI

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21
Q

Shark Reproduction

A
  1. Viviparity
    -eggs hatched within the dam’s uterus
    -Feti fed by placenta
    Ex: Bull, whitecap, lemon, blue, salmon, silver tip, hammerhead
  2. Oviparity
    -Eggs deposited in the ocean
    -Eggs hatch later if not consumed by predators
    Ex: Zebra, cat, horn sharks
  3. Ovoviviparity = Placental viviparity
    -Eggs hatch within the dam’s uterus, feti develop but NO Placenta present

Oviphagous: consuming unfertilized eggs and cannibalized cohort feti; few feti survive birth
Ex: great white, saw sharks, mako, etc.

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22
Q

Pathogenic bacteria

A

Aeromonas Salmonica
-disease in blacktop sharks

Flavobacterium spp
-Neurologic disease in bonnethead sharks

Vibrio carchariae
-Meningitis in sand tiger sharks, lemon sharks.

Bonnet head shark

-Fusarium solani
-Pustules erosion, ulcers in skin
-Fluid aspirate light microscopy, fungal culture
-Tx: anti fungal agents usually poor
-Poor prognosis

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23
Q

Lecture 3

A

Snakes

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24
Q

Snake anatomy, taxonomy, physiology

A

Taxonomy

-Reptilia

Anatomy

-No pectoral girdle
-Kinetic/mobile skull: no mandibular symphysis, flexible rami

Boids-internal pelvic girdle

-Spurs right and left side are remnants of the femur/pelvic limb

Cardiopulmonary

Heart
-3 chambered heart
-2 aortic arches
-Muscular ridge separates ventricle-shunts, shunt blood away from the lungs (important for anesthesia)

Trachea
-Open rings in squamata

Lungs
-Right only in most snakes
-Left vestigial
Paired boids
-Air sacs caudal part of the lung
-Tracheal lung in viperids, they can eat a large meal and not compromise the lungs

Urinary

-Paired kidneys
-No loop of henley: isothenuric urine (furosemide still works weird)
-No bladder

Renal portal system

-Blood draining caudal body passes through kidneys or liver before central circulation
-Effect on PK of drugs will vary with its renal or hepatic extraction rate
Most injections given in the front part of the body bc of this

Genital

-Testes (internal)
-Hemipenes
-Internal fertilization
-Oviparous: eggs laid and incubated, pythons
-Viviparus: young born live RATTLESNAKE and BOIDS

Hemipenal sacs

-Sexing
-Female: a couple scales
-Male: 8 scales deep

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25
Integument
Importance -Behavior -Thermoregulation -Sensory, protection, detection -Water/gas exchange Ecdysis -frequent when growing -requires humidity/bathing -One piece in healthy snakes -Shed teeth and tongue
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Snake husbandry
-Natural history -Photoperiod -Thermogradient POTZ (75-90F) preferred optimum temperature zone Basking areas Ridge-nose rattlesnake -Hide areas -Nutrition Manual restraint -Support body, especially boids -Venemous only experienced handlers, snake hook
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Conducting a PE
**Most important diagnostic tool** -Visual exam -Species ID, gender -Head to tail exam -Weight and measurements -Oral exam -Coelomic palpation -Serial exams in hospital BCS 1-5 1: Emaciated 2: Fair condition 3: Normal 4: Overweight 5: Obese Oral Exam -MMs: green mucosa = liver issues, lower PCV normal = less pink than mammals. Thick and sticky = dehydration. -Soft spatula to open mouth -Discharge from glottis or other areas -tongue flicking sign of good health -Plaque/stomatitis -Teeth -Parasites Eye -No eyelids -Cloudy eyes during shedding -Retained spectacle -Subspectacular abscess: swelling ventral window to allow drainage Nostrils, Ears, Head symmetry, Skin -Nostrils: symmetry, discharge -Tympanum (ear) no external ear -Skin: shedding = ecdysis. Dysedysis abnormal shedding, skin lesions, fungal disease, external parasites Coelomic Palpation -3-4 quadrant approach -Gall bladder: landmark for splenopancreas and distal stomach -Fluid -Enlarged organs -Foreign bodies -Eggs
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Diagnostic sampling and testing in snakes
Blood drawing/processing -Sterile prep, wear gloves -Ventral tail vein -0.5-0.8 ml/100 g BW-healthy -Heparin vs EDTA, heparin best -Lymph contamination -Whole blood and plasma banking Radiology -Adequate restraint -May need to do repeated segments -Make sure to label Cr, Cd, R, L Ultrasound -Sternal or dorsal recumbency -Image through ventral scales -Internal organs along longitudinal access -Know your anatomy
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Anatomy
Cranial 1/3 -Heart -Trachea and start of lungs Middle 1/3 -Air sac -Lungs -Stomach -Liver -Gall bladder -Spleen -Pancreas Caudal 1/3 -Air sac -SI -Colon -Kidneys -Repro tract (hemipenes mineralized) -Las pair ribs marks cloaca (boids pelvis)
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Common diseases due to poor husbandry
Low temperature -Regurgitation -Decreased GI motility and digestibility -Immunosuppression -Altered drug distribution -Pneumonia, bacterial fungal -Osteomyelitis -Necrotic stomatitis (mouth rot) -Thermal burns -Hyperthermia Burns -Very common -Mild erythema - skin damage - muscle necrosis -Fluid and protein loss -Bacterial infection Tx: aggressive fluid therapy, analgesics, antimicrobials -Wound care, debridement surgically -Dilute betadine, chlorhexidine, SSD cream, honey Regurgitation/vomiting -Handling shortly after a meal -Innapropriate nutrition -Cryptosporida -Foreign body Dysecdysis: shedding in pieces -No bathing water or humidity too low -Low temperature -Parasites -Retained skin -Skin wound: traumatic, surgical -Local/systemic dz Tx -Husbandry improvements -Provide soaking opportunities -Maintain at POTZ -Rocks and logs to rub on
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Parasites
External parasites -Cause of anemia -Vectors of blood parasites Tx -Reptile approved Permethrin and Cyfluthrin most effective and safe acarids -Manual removal (ticks, mites) Mites -Ophionyssus natricis -1 fertilized female = 1000s offspring -Eyes and skin folds common sites -C/S raised scales, dysecdysis, irritable, anorexia -Sign of poor sanitation -May transmit disease IBD Dx: low power microscopic exam Tx: -Clean housing -Newspapper substrate -Frontline, ivermectin injectable, oral or topical Endoparasites Collection of feces -Gentle pressure on colon -Colonic wash Protozoa -Trichomonads: flagellated, direct life cycle. -Entamoeba sp. Ameobiasis -Entamoeba invadens -Cysts, trophozoites. -Fecal contamination in environment -C/S: regurgitation, weight loss, bloody mucous diarrhea, sloughed mucosa, inflamed colon. -Necrotic ulcerative colitis and hepatic necrosis -Dx: fecal exam, stain cysts Lugol's iodine solution, PCR -Tx: metronidazole, antibiotics, antifungals. -Prevention: good hygiene and quarantine Cryptosporidiosis -Common problem in captive snakes -C. sepentis **Not zoonotic** -Stress predisposition -C/S: regurgitation, poor BCS, mid-body swelling, asymptomatic to fatal -Dx: Vomitus or feces, gastric lavage, biopsy, endoscopy, surgery -PCR, cytology, histopathology -Acid-fast stain, IFA Lesions: decreased diameter of gastric lumen, but increased overall diameter of the stomach -Histopath: gastric hyperplasia, fibrosis, organisms observed on epithelial cells -NO Tx effective -Separate infected stakes from healthy ones -5% ammonia or 10% formalin to disinfect, bleach not effective.
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Common non-infectious diseases in snakes
See previous
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Common parasitic and infectious diseases in snakes
Nematodes and Cestodes -Ascarids indirect life cycle, many IH -Kalicephalus spp. Hookworm, common in snake, DH -Strongyloides spp. direct life cycle, common -Cestodes Pentastomes **Zoonotic risk** -No truly effective treatment -Ivermectin at 5-10 times normal dosages -Endoscopically remove -Migratory larval/worms
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Prey item Parasites
Pseudoparasites
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Bacterial Diseases
-Usually opportunistic -Poor husbandry, temp, nutrition, lack of sanitation -Debilitated or injured predisposed -Gram (-) highest mortality -Anaerobes can be problematic -Caseous material vs mammalian liquid purulent material Salmonella spp **Zoonotic** -Fecal oral route -Capable of penetrating eggs -Poor hygiene -C/S: asymptomatic carriers, diarrhea, anorexia, lethargy -Enteritis, septicemia, vertebral osteomyelitis most common -Culture to rule out others -No treatment effective in permanently clearing out the organism -Good hygiene to prevent spread **Pregnant women, children, immunosuppressed should avoid** Snake fungal disease -Ophidiomyces ophiodiicola (Oo) -Emerging disease in wild snakes -Facial deformities, skin lesions Dx: skin biopsy, with history and PCR best -Tx: systemic antifungals, nebulization of terbinafine has promise Snake Inclusion Body Disease -Arenaviruses -Boids: boas, pythons, kingsnake, palm vipers. -Captive snakes -Acute CNS disease pythons -Chronic disease history boas: regurgitation, CNS Dx: Laparoscopy and biopsy -Transmission: unknown, suspected mites -Tx: quarantine, euthanasia
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Basic analgesia and anesthesia Surgery Basics
Analgesia -Have all anatomical structures considered critical for recognition of pain -Difficult to assess the effects of analgesia, signs of pain are subtle Drugs -Buprenorphine -Butorphanol -Ketoprofen -GI ulceration, decreased kidney function and sedation side effects Morphine -Preferred -Effective in bearded dragons, RES, crocs, anoles. -Prolong onset of action -Respiratory depression -Multimodal analgesia preferred ex: morphine, NSAIDs, local anesthetic Anesthesia -Propofol: 5-10 mg/kg -IV -Non-irritant -Rapid induction <1min -Ketamine + dexmedetomidine + buprenorphine IM, wait 15 minutes slowly increase gas, reverse with atipamezole -Midazolam can be added -Afaxalone Inhalants -Isoflurane preferred -Mask down in plastic tube after giving injectables -Ventilation throughout procedure -Doppler for BP -30-60 bpm -Heart beat post death for 24 hrs -End tidal CO2 -Thermoregulatory support Recovery -Stop inhalant -Continue analgesia -Reduce ventilation -AIR!! -Change to ambu bag -Post op: monitor, analgesia, POTZ -Fluid therapy
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Lecture 4
Chelonian Medicine
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Chelonian taxonomy, anatomy, and physiology Testudinadae: tortoises, adapted to arid and semiarid environments Marine turtles: 1) Dermochelydae 2) Cheloniidae
Ectothermic -External heat essential for all metabolic processes -75-95F -Low temp: immune function decreased, altered Pk drugs, decreased GI motility; normal flora overgrowth issues, cold stunning Shell -Carapace: top Plastron: bottom -Scute: keratinized plates -Overlay a dermis -Under deal CT is dermal bone -Compact and sandwich a middle layer of trabecular bone -Hatchlings: scutes called embryonic shields. Rings of new keratin, estimate age in younger tortoises -Lack intervertebral discs -8 cervical -10 trunk vertebrae fixed in place along with ribs-fused to dermal bone of carapace -30% of BW -No sternum, only tetrapod with the pectoral girdle internal to the ribs **Rhamphotheca or beak, no teeth** -Very few glands -Salt glands: diamondback terrapins and sea turtle -Musk glands in the cloaca -Mental or chin glands in some spp. males more prominent secrete pheromones, stinky Ear -No external ear -Tympanum, middle and inner ear -Eustachian tube connects middle ear with the throat GI tract -Tongue rich in salivary glands -Sea turtle: esophageal papillae. S-shape esophagus before the stomach -Cecal-like structure, proximal colon. Compartments nematodes common in this area -Should not deworm unless heavy burden Repro/Urinary -Phallus: penis, no urethra with it -All chelonians lay eggs -Kidneys: caudodorsal coelom. Renal portal system. No loop of henley = isosthenuric urine -Bladder: Ureters enter cloaca, not bladder. Tortoises large bladder, water conservation. Can absorb water and electrolytes through bladder CV system -3 chamber heart -Sinus venous - right atrium -Ventricular septa separation -Shunts **Complete tracheal rings**
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Secondary Sex characteristics
Female: convex plastron, warmer eggs = female Male: Concave plastron, cooler eggs = male
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Chelonian husbandry
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Conducting a PE
-Visual exam -Head to tail -Weight and measurements -Species ID, gender, age estimate -BCS -TPR -Hydration status -Oral exam -Coelomic palpation -Neurologic exam -Serial exams
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Diagnostic sampling and testing in turtles
Venopuncture **Jugular vein preferred** -Brachial vein: tortoises -Subcarapacial vein (lymph contamination) -Dorsal and ventral tail vein **Important to use the same vessel for comparison, samples can vary from different sites due to hemodilution with lymph** Hematology **Heparinized syringe** -EDTA will lyse RBCs in many chelonian species -Basophils more commonly seen -Nucleated RBCs -Heterophils Molecular diagnostics **very important tool** -Oral and cloacal swabs: ranavirus, mycoplasma, INC (intranuclear coocidia) , herpesvirus, adenovirus -Combine with cytology or histopathology Diagnostic imaging -Radiographs often poor contrast -No imaging technique is ideal for all situations -CT and ultrasound -Rigid endoscopy for internal fibropapilloma **spirochid trematode lesions** -Fibropapilloma: herpes virus, INIBs
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Common diseases due to poor husbandry
Toxicities -Ivermectin (never give to a turtle) -Aminoglycosides -Vitamin A/D -Organochlorines: ear abscesses -Oil ingestion -Oak toxicity -Azalea toxicity **Fenbendazole toxicity: bone marrow suppression** -Tx: similar to other species
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Other diseases/conditions
-Cloacal prolapses: Tx: surgery/amputation phallus, or lubrication sugar to reduce inflammation -Trauma: HBC, propeller injuries, predator injuries, intentional. Shell trauma -Radiographs -Asses for pelvic fractures, egg laying, vital structures -Stabilize turtle, mannitol for head trauma -Treat open wounds -Wire, bra hooks, zip ties, bone cement etc.
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Common non infectious diseases
Metabolic Bone Disease -Common problem -Secondary nutrition hyperparathyroidism (SNHP) -Secondary renal hyperparathyroidism -Hypervitaminosis D3 Hypertrophic osteopathy SNHP -Rapid growing young turtles -Low Ca:P -Inadequate UVB C/S: Anorexia, lethargy, unable to raise body, muscle fasciculations, skeletal deformities Dx: radiographs: decreased mineralization, pathological fractures. -Biochemistry: elevated PO4, decreased total/ionized Ca Tx: diuresis w fluids, calcium supplementation, phosphate binders, direct sunlight, minimize handling. -Fracture: external computation/bandaging Vitamin A deficiency -Common in young, growing -Respiratory and ocular adnexal structures affected -Ear abscesses, conjunctivitis, palpebral edema -Loss of ciliated epithelial cells in respiratory tract. C/S: anorexia, swollen and closed eyes, ureter obstruction from squamous metaplasia. Tx: diet change, oral vitamin A, topical and systemic antibiotics -surgical treatment is palliative Vitamin A toxicosis -Usually occurs 2-4 weeks after a vitamin A injection -Skin peel/sloughing in sheets -Tx: wound care and antibiotics -Oral supplementation and diet improvement best GOUT Causes: -Renal impairment, dehydration, excess protein and purines in diet -Aminoglycoside, toxicity C/S: anorexia, lethargy, enlarged joints, internal organ nodules, visceral gout -Dx: uric acid levels increased, biopsy, aspirate, radiographs. -Tx: catch early, eliminate primary problem, fluids, allopurinol colchicine, surgical removal of acid from joints. -Poor prognosis if advanced Urolithiasis -Common in tortoises -Uric acid, struvite, and calcium -Dehydration, excess protein diet -Bacterial or fungal cystitis Tx: coelotomy/cystotomy inguinal approach
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Common parasitic infections
Ticks -Amblyomma most common -Vectors of other parasites -Inguinal and axillary regions Tx: Permethrin, cyfluthrin, manual removal Leeches -Manual removal, fresh water soaks Protozoal -Immunosuppressed lead to increased in normal flora flagellates and ciliates **Entamoeba spp, only one pathogenic naturally**
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Ameobiasis
-Mortality in all reptiles, nakes **Tortoises may be asymptomatic carriers** -If symptomatic: severe depression, watery diarrhea, dehydration -Thickened and edematous duodenum with necrotic mucosa -Necrosis of common bile duct and gall bladder, diffuse hepatic necrosis Dx: Psuedomembrane like material in feces-necrotizing colitis histologically -PCR -Biopsy of liver -Tx: supportive care, metronidazole, antibiotics, antifungals
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Intranuclear Coccidiosis
-C/S: non-specific, anorexia, lethargy -Anemia, leukocytosis, low Na, hyperglycemia, increased uric acid. **INC and mycoplasma in nasal cavity** **High mortality and morbidity** Dx: biopsy of internal organs, cloacal swabs and oral with PCR -Nephritis, hepatitis, enteritis, -No oocysts in feces -Tx: Panazuril, Toltrazuril, none proven effective
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Other parasitic and infectious diseases Which are commensal parasites that aid in digestion?
Nematodes, trematodes -Ascarids -Strongyles, hookworms **Oxyurids-Pin worms** commensals, may aid in digestion Bacterial Diseases -Usually opportunistic: poor husbandry, malnutrition, lack of sanitation. -Gram (-) most common, anaerobes **Casseous abscesses** Salmonella spp. -Zoonotic -Red eared sliders-280,00 cases human salmonellosis in the 70s, starting to see this again -Asymptomatic carriers -Enteritis, septicemia most common -Poor husbandry/hygiene -Pregnant, immunocompromised avoid contact Mycoplasmosis -URTD -Free ranging tortoises in USA -C/S: ocular and nasal discharge -Chronic infection, often subclinical, intermittent Dx: PCR, serology, culture Tx: Fluoroquinolones, tetracyclines systemic/intranasal Mycotic diseases -Predisposing factors: suboptimal POTZ, excess humidity, poor sanitation, over crowding, underlying viral disease Dx: bronchoscopy, biopsy, serology, culture. Tx: resection isolated infections, systemic anti fungal ITRACONAZOLE, voriconazole, topical therapy Viral Diseases -Herpes virus: necrotizing stomatitis. Tx: Acyclovir, supportive care -Fibropapillomas in sea turtles -Iridovirus (Ranavirus) -Adenovirus -EM: fungal caused shell lesions
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Analgesia and Anesthesia
Preferred anesthetics -Propofol -Dexmedetomidine -Midazolam -Alfaxalone -Atipamezole -alpha 2 antagonist -Flumazanil - midazolam benzodiazapine antagonist Monitor -Change to ambu bag -POTZ -Fluid therapy
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Surgery basics
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Lecture 5
Marsupials and Insectivores Phylagenetic tree -Therian mammals: Eutherians & Marsupials -Prototherian mammals
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Anatomy & physiology of the common marsupial reproductive tracts
Marsupials: mammals with marsupium/pouch to protect offspring Reproductive tract Female -Double cervices -Multiple vaginas -Common birth canal of uterus -Marsupium: pouch, epipubic bones, bilateral bones for support of the marsupium -Fetus has prolonged attachment to teat within the marsupium Male -Bifid or double-ponged glans penis -Scrotum has cranial placement to penis -Penis caudally oriented 3 life stages of offspring -Concurrent life stages -Stage 1: joey goes from cloaca to the nipple in the pouch -Stage 2: Hangs onto nipple for dear life -Stage 3: Baby #1 outside the pouch, baby #2 begins its nipple bound journey Marsupial Fetus -Advanced thoracic limb development -Parturition and migration from vagina to marsupium -Prolonged attachment to teat Skeletal Adaptations -Macropods: lack patellas -Epipubic bones: attached to craniodorsal aspect of pubic bones to support marsupium
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Virginia opossum - Didelphis virginiana
Native to N. America Prehensile tail Large liters Increased number of teeth vs. placental mammals
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Characteristics of specific species of marsupials and insectivores commonly presented as pets
Sugar gliders - Petaurus breviceps Anatomy -Marsupium -Patagium: skin beternn thoracic and pelvic limbs, used during gliding -Epipubic bone -Syndactyly foot @ digits 2 & 3 -Hair comb -Male: multiple scent glands -Nocturnal large eyes for night vision. Reproduction 1 male per multiple females -16 days gestation, 3 months ~ 70 days, postpartum in the pouch -Litter size: 2 -Social mammals require attention from owner or another pen mate -Males: urinate at proximal region of penis. -Amputation of glans penis common when penile trauma or paraphimosis
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Basic clinical techniques necessary for evaluation common pet marsupials and insectivores
-PE, blood collection, Tx plans similar to other mammalian -Often require physical restraint to allow thorough PE -Similar to a hamster or gerbil: control head to avoid bite injuries -Restraint by scruff on head/neck -Thorough exam: ophthalmic, otic, oral cavity, dental exam, integument, marsupium, urogenital, cardiovascular, respiratory, musculoskeletal, attitude/behavior. **annual dental check** Clinical Pathology -Venipuncture sites: jugular vein or cranial vena cava, femoral vein. **Anesthesia required** -Cephalic, lateral saphenous -use 05ml to avoid collapse -<1ml heparinized blood/syringe 25 or 27 gauge needle Fluid therapy -Avoid patagium: poor dispersion and discomfort to gliders Radiology -General anesthesia required -Unfold patagium to improve contrast
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Unique nutritional requirements of marsupials and insectivores
**Omnivorous** -Best diet: avian pellet feed, lorry nectar, fruits and vegetables, cooked eggs, insects (gut loaded) -Commercial insectivore diet 1:1 ratio -Obesity prone species Digestion -Enlarged cecum, microbial fermentation of complex polysaccharides in gum (tree zap) -Lengthened 4th digit on manus to extract insects -Consume 17% of BW in the wild -Captive sugar gliders use less energy Habitat -Require nesting box -Minimum cage size: 20x20x30" -Avoid mesh screening -Use non-toxic wood
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Common Diseases of sugar gliders and African hedgehogs
Degenerative: dental disease and incisors Anatomical, anomaly, acquired. Rectal prolapse, impaction of paracloacal glands. Metabolic: cystitis, crystaluria, urolithiasis, urinary tract obstruction. Nutritional: hypocalcemia leading to tremors/seizures. Infectious, iatrogenic: Salmonella spp., Leptospira zoonoses Trauma, toxin: self mutilation, cage mate aggression Congenital Hemological DAMN ITCH
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Hedgehog
African pygmy hedgehog (Atelerix albiventris) Anatomy -Brachydontic type teeth -Bilateral clavicles, plantigrade gate -Nocturnal -300-600 g -5-7 years life span -Abdominal testes, induced ovulators -Gestation 34-37 days, 3-6 offspring -Neonates have protective coating over spines at parturition, lost within 24 hours -Weaning at 30-40 days of age -Sexually mature 2-3 mts of age Diet -Wild: insects, semi-vertebrates, carrion -Captive: obesity prone -Insects desirable, boiled eggs with shells -Once daily feeding at night hours, evening exercise PE, etc -Wear gloves -Anesthesia required Isoflurane or sevoflurane -Venipuncture: jugular v., vena cava. -Maximum 1% of BW blood draw -Post venipuncture: digital pressure Radiographs -Require general anesthesia -Clavices present Therapy -Early treatment best -Small body can quickly lead to starvation and dehydration -Intraosseous fluid therapy -Maintenance volume: 100 mls/kg/day Common diseases -Ocular proptosis, "wobbly hedgehog syndrome" -Obesity, neoplasia, SCC, mammary gland tumors, lymphosarcoma -Dental abscesses, unsanitary bedding -Salmonella, rabies, herpex simplex 1, Foot and Mouth disease carrier.
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