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exam 1 Flashcards

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1
Q

true or false? you need an electroporation cuvette for heat shock transformation of E.coli

A

false

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2
Q

Which of the following are parts of an adaptor used for preparation and sequencing of genomic DNA fragments

A) a sequence that anneals to an oligoneculeotide primer

B) a sequence that anneals to an sequencing primer to prime the sequencing reaction

C) a unique barcode sequence used to tag a genomic library

A

all of the above

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3
Q

TA cloning is a PCR based cloning true or false

A

True

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4
Q

you extracted RNA from a drought stressed plant leaf. you are interested in cloning only drought stressed cDNAs. what type of primer will you use at the very beginning after the sythesis of mRNA?

A

Gene specific primer

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5
Q

T4 polynucleotide kinase is used in Dr. Sanger’s method of DNA sequencing. true or false

A

false

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6
Q

in crisper system for use in biotech can the sgRNA and Cas9 gene be cloned in the same plasmid

A

yes

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7
Q

neoschizomers are restriction enzymes that

A

can bind at the same recognition site but cleave at differnt sites

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8
Q

a ddNTP has

A

a H group at the 3’ carbon

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9
Q

what is true about the oxidation step during primer synthesis

A

this step makes the bonds between the neucleotides stronger

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10
Q

at the time of the gene synthesis by primers how can you seal the nicks in the strands of DNA

A

by adding DNA ligase

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11
Q

is a chemical blocking group attatched to the 3’ carbon of the sugar moiety during pyrosequencing ?

A

no

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12
Q

what is true about capping during primer sythesis

A

capping is done to prevent prmers differing in lengths

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13
Q

luciferase+_____–> light

A

ATP

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14
Q

what is pyrosequencing?

A

a cleaved beta and gammma phosphate from dNTp molecule

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15
Q

MALDI-TOF analysis of protein molecules are represented by ?

A

mass to charge ratio

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16
Q

you want to repair the ends of DNA repair means the addition of nucleotides what type of enzyme do you use?

A

DNA polymerase

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17
Q

in colony immunoassay, first cells are lysed to release___ bound on the membrane

A

protein

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18
Q

you are identifying disease biomarkers with human protein microarray. what should be spotted onto the slide?

A

human proteins

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19
Q

what enzyme is used to “paste” your gene of intrest fragment into a plasmid cloning vector?

A

ligase

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20
Q

is DMT group part of a phosphoramidite?

A

yes

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21
Q

you are using “sandwich’ style protein microarray to study post translational modifications. should the proteins be labeled with flourescent tags?

A

no

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22
Q

you can analyze millions of transcripts using RNAseq? true or false

A

true

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23
Q

during primer synthesis how can you remove a DMT group?

A

by addition of TCA

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24
Q

in CRISPR invadiong foreign DNA have a sequence similarity with____

A

Crispr loci

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25
which sequencing method has the lowest accuracy
Single molecule sequencing
26
the ccdB gene used in recombination cloning (gateway cloning) is a
gene that produces toxic compound
27
you extracted DNA then what is the first step in shotgun sequencing?
break genomic DNA
28
shine dalgarno sequence is
a ribosomal binding site in prokaryotes
29
in crisper system the invading DNA must have a
PAM sequence
30
in qPCR. if sample A has a Ct value of 20 and sample B has a Ct value of 40 in the same qPCR experiment can you canclude sample A has more DNA than sample B
yes
31
a kinase
transfers a phosphate group to a DNA
32
in blue white colony screening
LacZ gene encodes for beta galactosidase in the presense of X-Gal
33
activation and coupling during primer synthesis are mediated by
tetrazole
34
Dr. venter performed comprehensive construction of DNA libraries from all microorganisms from a region in Saragasso sea. this type fo study is known as
metagenomic study
35
During cDNA synthesis you extracted total RNA and by mistake added RNAseh instead of reverse transcript. what will happen?
my RNA will be degraded and i will not be able to continue cDNA synthesis
36
during the primer synthesis the purpose of adding a DMT group to a nucleotide is
to prevent unlinked nucleosides reacting non specifically
37
you are using a yeast two hybrid system. you have 100 yeast strains containing DNA fragments as prey library. can you mix all prey yeast strains into a tube and have the bait yeast strains mate with the prey
yes
38
your goal is to synthesize a gene. you synthesized oligonucleotides what should be now the immediate next step
increase temperature to 95*C for denaturation
39
which of the following enzymes is able to synthesize another strand of DNA from a template DNA molecule
Klenow (DNA) polymerase
40
the SYBR green dye used in qPCR can bind with both single and double stranded DNA
no
41
what is true about metagenomic library construction
you need to perform shotgun sequencing
42
what is detriylation?
the removal of DMT from necleoside during primer synthesis
43
you can use pUC 19 for blue white colony screaning
true
44
during synthesis of oligonucleotides the amino group is removed all nucleotides except
T
45
find a restriction enzyme producing blunt ended DNA fragment
Pvull
46
pyrophosphate +______ =ATP
ATP sulfrylase
47
gel is needed for qPCR
false
48
in crispr the components of single guide RNA is crRNA and _______
tracrRNA
49
in tandem affinity purification procedure a plasmid DNA is used with two tags. which sequence is inserted between the two tags?
protease clevage site
50
a 2D gel elecrephoresis system separates protein on the bands of elecgtric gradient an ____ gradien
pH
51
what site/genetic element is an essential component of phage DNA to construct a phage library
COS
52
what is the realistic proportion of mRNA in total RNA sample?
3%
53
what is the role of SEC complex in protein transport in gram positive bacteria
creates a passage thx which entire protein and signal peptide passes
54
what is the prinbrow box
TATAAT
55
a bacteriophage life cycle goes through lysogeny and lytic cycle after it infects E. coli which is true?
in lysogeny phage DNA gets integrated in bacterial genome and maaintained in bacterial genome
56
what are the number 5 and 3 in 5' and 3' represent when we describe the orientation of DNA
number on the carbon atom of the nucleotide
57
biotin streptabin conjugate is used to detect numerous biomolecules including nucleic acids
true
58
all of the antibiotics below inhibit protein sythesis and kill cells which one does not inhibit protein synthesis but still kills the cell
ampicillin
59
at the time of labeling a probe it is nescessary to boil the probe. why?
boling denatures the probe and only a single stranded denatured probe can hybridize effectively with target DNA
60
what is southern blot hybridization
the gene of intrest (probe) is labled and total genomic DNA is loaded onto the gel
61
which enzyme is used to "paste" GOI fragment in a cloning vector
ligase
62
find a restriction enzyme producing blunt ended DNA fragments
PvuII
63
Neoshizomers are RE that can >>>>
bind at the same recognition site but cleave at a differnt site
64
TATA box
promoter region
65
RNA polymerase
binds to promoter region in the DNA to initiate transcription
66
if a sequence of a template strand of a DNA is CAATGA the sequence of mRNA synthesized will be
GUUACU
67
an mRNA molecule is successfully alternitively spliced to form two functional coding mRNA. alternitive splicing can generate two:
differnt types of mRNA with differnt exons
68
arrange the following events in transcription and translation RNA polymerase binds to DNA ribosome is attatched to to mRNA mRNA sythesis
RNA polymerase binds toDNA -> mRNA synthesis-> ribosome is attached to mRNA
69
and mRNA molecule has a unique feature which is absent in tRNA or rRNA
a poly a tail
70
in blue white colony screening whats the role of IPTG
it inactivated repressor protein
71
which of the following is not a part of the trnscription factor complex
TFIIZ
72
as soon as proteins are synthesized from ribosome-mRNA complex the protein are loaded into the
rough endoplasmic reticulum
73
what is the disadvantage of pBR322 cloning vector
very few cloning sites
74
sometimes the functional genetic complementation is used at the time of gene cloning which is true of functional genetic complemntation
you can study gene response for host cell specific enzyme activity, make DNA library, transform host cells with DNA library all of the above
75
what is the smallest insert capacity to largest?
plasmids>bacteriophage lambda>BAC>YAC
76
what does deoxyribose look like
2'H 3' OH
77
what is the finction of RNASeH
digest any type of RNA double or single stranded
78
what is the disadvantage fo shittle vector
they can be lost from the host cell
79
which one is the worst cloning vector for human genome sequencing
plasmid
80
which is true of dynabead
have oligodT attached to it
81
what is the role if kinase
transfer phosphate to donor molecules to substrates through phosphorylation
82
is it posible for plant to have larger genome than humans
yes statment is true in some case but not all
83
Which is true about klenow frag.
its a product of E. coli DNA polym
84
Which is NOT true about pBR322 plasmid vector
has a lac z gene
85
What's true about gene cloning by functional complementation technique we studied in class
A. All correct; need normal and defective E. coli strain, need genomic DNA library w. Goi cloned, need to grow cells on min. Medium
86
Which enzyme is exclusively used in RNA manipulation but not in DNA manipulation
A. Poly A polymerase
87
How do you develop EST library?
By random sequencing of cDNAs into a CDNA library
88
You work for USDA and interested in gene expression in corn plants. You can perform
A. Microarray and northern blot

biotech csun (1 decks)

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