Exam 1 (Blood Smears)

Question 1

What dyes does a Wright stain contain?

Answer 1

Eosin and methylene blue

Question 2

What dyes does a Wright-Giemsa’s stain contain?

Answer 2

Methylene blue azure in addition to eosin and methylene blue

Question 3

What is the purpose of methanol?

Answer 3

Fixes smear to slide

Question 4

What is the purpose of the buffer?

Answer 4

Controls the pH of the staining solution

Question 5

What are the requirements for a good smear

Answer 5

1/2 to 3/4 length of slide not extending past last 1/4 of slide, smooth transition from thick to thin ending in a feathered edge, proper ID, WBCs evenly distributed, feathered edge at least 3 LPF in width where RBCs barely touch but don’t overlap.

Question 6

What are the advantages of EDTA for smears?

Answer 6

Can make multiple slides, usually prevents platelet clumping,

Question 7

What is the normal angle of the spreader slide in making blood smears?

Answer 7

30 - 40 degrees

Question 8

What is the pH of aged distilled water?

Answer 8

6.4-6.8

Question 9

How do you correct by clumping caused by EDTA?

Answer 9

Use sodium citrate

Question 10

What is the pH of aged distilled water?

Answer 10

6.4-6.8

Question 11

What are the features of a well stained slide?

Answer 11

Macroscopically pink to purple in color; microscopically - RBCs orange to salmon pink, WBC nuclei purple to blue, eosinophils bright orange refractive granules, cytoplasm of neutrophils pink to tan with violet or lilac granules

Question 12

What are the features of a well stained slide?

Answer 12

Macroscopically pink to purple in color; microscopically - RBCs orange to salmon pink, WBC nuclei purple to blue, eosinophils bright orange refractive granules, cytoplasm of neutrophils pink to tan with violet or lilac granules

Question 13

What is the result of platelet clumping?

Answer 13

Pseudoleukocytosis, falsely low platelet counts,