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BIOL458 - Microbiology Lab > Exam 2 Differential Media > Flashcards

Exam 2 Differential Media Flashcards

(52 cards)

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1
Q

Blood Agar tests for

A

Hemolysis type

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2
Q

Blood agar substrate

A

Blood

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3
Q

Blood agar indicator

A

Blood

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4
Q

Blood agar positive result

A

Media clears for beta hemolysis, color change for alpha hemolysis

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5
Q

Blood agar negative result

A

Growth with no color change is gamma hemolysis

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6
Q

Chocolate agar tests

A

Fastidious organisms

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7
Q

Chocolate agar substrate

A

lysed RBCs

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8
Q

Chocolate agar positive result

A

Growth on chocolate agar and not lb agar means fastidious

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9
Q

Chocolate agar negative result

A

Growth on both means not fastidious

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10
Q

Phenol Red Broth tests for

A

fermentation, deamination, and gas production

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11
Q

Phenol red broth substrate

A

Lactose

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12
Q

Phenol red broth indicator

A

Phenol Red, Durham tube

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13
Q

Phenol red positive result

A

Yellow broth means fermentation, pink broth means deamination, and bubble means gas production

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14
Q

Phenol red negative result

A

red broth means nothing

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15
Q

MR-VP tests for

A

differentiates between mixed-acid pathway and 2,3-butanediol pathway for fermentation

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16
Q

MR-VP substrate

A

glucose

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17
Q

MR-VP indicator

A

Methyl red, VP reagents

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18
Q

MR-VP positive result

A

Red is positive for both; MR is mixed acid, VP is 2,3-butanediol

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19
Q

MR-VP negative result

A

colorless

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20
Q

MR-VP added reagents

A

MR is methyl red; VP is VP reagents A and B

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21
Q

Catalase test tests for

22
Q

Catalase substrate

23
Q

Catalase indicator

24
Q

Catalase positive result

25
Catalase negative result
no bubbles
26
Oxidase test tests for
Cytochrome c oxidase
27
Oxidase substrate
TMPD
28
Oxidase indicator
TMPD
29
Oxidase positive result
purple
30
Oxidase negative result
colorless
31
Nitrate Broth tests for
Nitrate reduction
32
Nitrate broth substrate
Potassium nitrate
33
Nitrate broth indicator
Durham tube, NItrate broth reagents A and B, zinc
34
Nitrate broth positive result
bubble means denitrification, red color after A and B means nitrite formation, no color after everything means other nitrate reduction
35
Nitrate broth negative result
red after zinc means no nitrate reductions
36
NItrate broth added reagents
Nitrate Reagents A and B, zinc
37
Citrate Agar tests for
Citrate metabolism
38
Citrate agar substrate
Sodium citrate
39
Citrate agar indicator
Bromothymol Blue
40
Citrate agar positive results
Growth with blue color change or colorless growth
41
Citrate agar negative results
no growth
42
TSI tests for
Glucose or Lactose fermentation, sulfur reduction, gas production
43
TSI substrate
Lactose, Sucrose, Glucose, Sodium Thiosulfate
44
TSI indicator
Ferric Ammonium Citrate, Phenol Red
45
TSI positive results
Yellow means fermentation, pink means deamination, black means sulfur reduction, space under butt means gas production
46
TSI negative results
red means nothing
47
SIM tests for
sulfur reduction, indole production, motility
48
SIM substrate
Sodium thiosulfate
49
SIM Indicator
Ferrous ammonium sulfate, Kovac's reagent
50
SIM positive results
Black means sulfur reduction, turbidity means motility, red Kovac's reagent means indole production
51
SIM negative results
colorless means nothing, clear means no motility
52
SIM added reagents
Kovac's reagent

BIOL458 - Microbiology Lab (3 decks)

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