Exam 2 Practical

Question 1

Name this pathogenic trypanosomatid that can be found in blood and tissues

Answer 1

Leishmania spp (extracellular)

Question 2

Name this pathogenic apicomplexa that can be found in blood and tissues

Answer 2

Plasmodium ovale

• Infects larger-sized RBCs
• Stippling present
• Fimbriated
• Schizont contains 6-14 merozoites

Question 3

Name this nonpathogenic protozoa that can be found in the intestines

Answer 3

Endolimax nana troph

Question 4

Name this pathogenic trypanosomatid that can be found in blood and tissues

Answer 4

Trypanosoma brucei

• Small kinetoplast

Question 5

Name this nonpathogenic protoza that can be found in the intestines

Answer 5

Chilomastix mesnili cyst

Question 6

Name this nonpathogenic protozoa that can be found in the intestines

Answer 6

Pentatrichomonas hominis troph (no cyst form yet identified)

Question 7

Name this nonpathogenic protozoa that can be found in the intestines

Answer 7

Chilomastix mesnili troph

Question 8

Name this pathogenic flagellate that can be found in blood and tissues

Answer 8

Trichomonas vaginalis troph

Question 9

Name this pathogenic apicomplexa that can be found in the intestines

Answer 9

Sarcocystis hominis and S. suihominis

(Autofluoresces)

Question 10

Name this pathogenic apicomplexa that can be found in the intestines

Answer 10

Cyclospora cayetanensis oocysts

(Modified-acid fast alters morphology, ghost cells don’t stain)

Question 11

Name this pathogenic trypanosomatid that can be found in blood and tissue

Answer 11

Leishmania spp (intracellular)

Question 12

Name this nonpathogenic protozoa that can be found in the intestines

Answer 12

Enteromonas hominus

Question 13

Name this pathogenic apicomplexa that can be found in blood and tissues

Answer 13

Plasmodium falciparum

• Infects normal-sized RBCs
• No stippling
• Primarily ring forms seen in RBCs
• Multiple rings/RBC
• Banana shaped gametophytes

Question 14

Name this pathogenic amoebae that can be found in blood and tissues

Answer 14

Balmuthia mandrillaris cyst

Question 15

Name this nonpathogenic protozoa that can be found in the intestines

Answer 15

Iodamoeba bütschlii cyst

Question 16

Name this pathogenic apicomplexa that can be found in blood and tissues

Answer 16

Babesia spp

To differentiate from Plasmodium, Babesia produces racket-shaped merozoites, ring forms, an extracellular parasites are seen)

Question 17

Name this pathogeic apicomplexa that can be found in blood and tissues

Answer 17

Toxoplasma gondii bradyzoite

Question 18

Name this nonpathogenic protozoa that can be found in the intestines

Answer 18

Iodamoeba bütschlii troph

Question 19

Name this nonpathogenic protozoa that can be found in the intestines

Answer 19

Restortamonas fragilis troph

Question 20

Name this pathogenic apicomplex that can be found in blood and tissues

Answer 20

Toxoplasma gondii tachyzoite

Question 21

Name this pathogenic intestinal apicomplexa and the type of form (troph vs. cyst)

25-30µm

Answer 21

Cystoisospora belli

(Note ellipsoidal shape containing 1 sporoblast)

Question 22

Name this fungi that can be found in the intestines

Answer 22

Microsporidia

Question 23

Name this pathogenic amoeba that can be found in blood and tissues

Answer 23

Acanthamoeba spp cyst (double walled)

Question 24

Name this pathogenic amoebae that can be found in the intestines

Answer 24

Entamoeba hartmanni cyst

Question 25

Name this pathogenic apicomplexa that can be found in blood and tissues

Answer 25

Plasmodium malariae - Infects normal to slightly larger RBCs - No stippling - May see "band form" trophs

Question 26

Name this pathogenic amoebae that can be found in the intestines

Answer 26

Entamoeba harmanni troph

Question 27

Name this pathogenic amoebae that can be found in blood and tissues

Answer 27

Acanthamoebae spp troph (bull's eye; identical to Balamuthria mandrillaris troph)

Question 28

Name this pathogenic apicomplexa that can be found in the intestines 4-6µm

Answer 28

Cryptosporidium spp oocysts (Note, how the oocysts have four crescent-shaped sporozoites present)

Question 29

Name this pathogenic flagellate that can be found in the intestines 4-12µm

Answer 29

Dientamoeba fragilis cyst

Question 30

Name this nonpathogenic protozoa that can be found in the intestines

Answer 30

Restortamonas intestinalis cyst

Question 31

Name this pathogenic aplicomplexa that can be found in blood and tissues

Answer 31

Plasmodium vivax - Ring forms present (amoeboid trophs) - Infects larger-sized RBCs - Stippling present - Schizont contains 12-24 merozoites

Question 32

Name this pathogenic amoebae that can be found in the intestines 15-20µm

Answer 32

Entamoeba histolytica troph (Note the RBCs w/in the cytoplasm; left pic is a trichrome stain)

Question 33

Name this pathogenic ciliate that can be found in the intestines 40-200µm

Answer 33

Balantidiium coli troph (Note kidney bean-shaped macronucleus; right pic is a wet mount, note cilia)

Question 34

Name this nonpathogenic protoza that can be found in the intestines

Answer 34

Endolimax nana cyst

Question 35

Name this pathogenic amoebae that can be found in the intestines 12-15µm

Answer 35

Entamoeba histolytica cyst

Question 36

Name this pathogenic amoebae that can be found in blood and tissues

Answer 36

Naegleria fowleri

Question 37

Name this pathogenic trypanosomatid that can be found in blood and tissues

Answer 37

Trypanosoma cruzi - Large kinetoplast = Large badonk-a-donk

Question 38

Name this pathogenic flagellate that can be found in the intestines 12µm

Answer 38

Giardia intestinalis troph

Question 39

Name this miscellaneous organism that can be found in the intestines

Answer 39

Blastocystis spp

Question 40

Name this pathogenic flagellate that can be found in the intestines 10µm

Answer 40

Giardia intestinalis cyst | (Trichrome stain\)