Exam 2: RNA Processing Flashcards Preview

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Flashcards in Exam 2: RNA Processing Deck (55):
1

What types of cells are we mainly talking about in this flashcard set?

EUKARYOTES! For our purposes, there is NO mRNA PROCESSING in prokaryotes. Prokaryotes do process rRNA and tRNA, but that is outside the scope of this flashcard set

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2

How are final rRNA and tRNAs different from transcribed pre-rRNA and pre-tRNAs?

They are post-translationally modified by cleavege from precursors, other covalent modifications.

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3

Where a pre-rRNAs cleaved to form functinoal RNAs?

In the nucleolus (shown in purple)

 

Further reading:

The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli are made of proteins and RNA and form around specific chromosomal regions.

Three major components of the nucleolus are recognized: the fibrillar center (FC), the dense fibrillar component (DFC), and the granular component (GC).[2] The DFC consists of newly transcribed rRNA bound to ribosomal proteins, while the GC contains RNA bound to ribosomal proteins that are being assembled into immature ribosomes.

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4

tRNAs are processed by?

Ribonuclease (commonly abbreviated RNase) is a type of nuclease that catalyzes the degradation of RNA into smaller components. Ribonucleases can be divided into endoribonucleases and exoribonucleases.

Made of RNA and protein, both parts functional, but RNA is the catalytic agent. Because of this, ribonucleases are a subclass of ribozymes

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5

What type of macromolecule provides the catalytic ability of a ribozyme

RNA.

tRNA ribonucleases fall under this category, they contain both RNA and protein subunits. However, RNA contains the catalytic ability 

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6

What is the difference between processing of mRNA in prokaryotes and eukaryotes?

mRNA in eukaryotes undergo many processing steps like 5' capping, 3' poly(A) tail, intron splicing (removal) before export from the nucleus. Prokaryotes do not undergo these modifications, infact in prokaryotes transcription and translation may occur simultaneously!

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7

How many proteins does each mRNA code for?

Generally only one in eukaryoes, many in prokaryotes

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8

Why do the ends of eukaryotic mRNAs need special processing (5' cap, 3' poly(A) tail)?

RNA polymerase in eukaryotes does not terminate as precisely as it does in prokaryotes 

9

Why are 5' caps added in eukaryotic mRNAs but not in prokaryotes?

Prokaryotes transcribe and translate simultaneously, their RNA need not be as stable because they don't need the extra transport time to get out of the nucleus.  Capping marks mRNA for export and prevents accidental degredation by exonucelases. It also is required for translation and can be used to control expression.

10

Structure of the 5' cap and funtion

The 5' cap is a guanine nucleotide connected to mRNA via an unusual 5′ to 5′ triphosphate linkage. This makes it appear chemically similar to the 3′ end of an RNA molecule (the 5′ carbon of the cap ribose is bonded, and the 3′ unbonded), providing significant resistance to 5′ exonucleases (prevents degredation).

 

Further reading:

In eukaryotes, the 5′ cap (cap-0), found on the 5′ end of an mRNA molecule, consists of a guanine nucleotide connected to mRNA via an unusual 5′ to 5′ triphosphate linkage. This guanosine is methylated on the 7 position directly after capping in vivoby a methyltransferase. It is referred to as a 7-methylguanylate cap, abbreviated m7G.

In multicellular eukaryotes and some viruses, further modifications exist, including the methylation of the 2′ hydroxy-groups of the first 2 ribose sugars of the 5′ end of the mRNA. cap-1 has a methylated 2'-hydroxy group on the first ribose sugar, while cap-2 has methylated 2'-hydroxy groups on the first two ribose sugars, shown on the right. The 5′ cap is chemically similar to the 3′ end of an RNA molecule (the 5′ carbon of the cap ribose is bonded, and the 3′ unbonded). This provides significant resistance to 5′ exonucleases.

Nuclear export of RNA is regulated by the cap binding complex (CBC), which binds exclusively to capped RNA. The CBC is then recognized by the nuclear pore complex and exported. Once in the cytoplasm after the pioneer round of translation, the CBC is replaced by the translation factors eIF-4E and eIF-4G. This complex is then recognized by other translation initiation machinery including the ribosome.

Capping prevents 5′ degradation in two ways. First, degradation of the mRNA by 5′ exonucleases is prevented (as mentioned above) by functionally looking like a 3′ end. Second, the CBC and eIF-4E/eIF-4G block the access of decapping enzymes to the cap. This increases the half-life of the mRNA, essential in eukaryotes as the export and translation processes take significant time.

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11

Describe the process of capping a eukaryotic mRNA

First, a phosphatase removes one phosphate from the very 5’ end (the 5' end was added with a NTP (nucelotide triphosphate, and so has three phosphates at the very end. Removing one leaves two phosphates attatched to the 5' end, now a NDP).

Next, guanylyl trasferase adds GMP in an unusual 5’-5’ triphosphate linkage (this addition is not the same as a regular addition, it is added in the 5' to 3' direction, which RNAPol II is not capable of. Notice it is catlysed by a different protein, guanylyl trasferase. GMP is a mono phosphate, so added to the NDP makes a triphosphate 5'-5' linkage).

The G is then modified by methyl transferasese, which add one or more methyl groups (see below for further reading)

 

The 7-nitrogen of guanine is methylated by mRNA (guanine-N7-)-methyltransferase, with S-adenosyl-L-methionine being demethylated to produce S-adenosyl-L-homocysteine, resulting in 5'(m7Gp)(ppN)[pN]n (cap-0);

Cap-adjacent modifications can occur, normally to the first and second nucleotides, producing up to 5'(m7Gp)(ppN*)(pN*)[pN]n (cap-1 and cap-2);[7][8]

If the nearest cap-adjacent nucleotide is 2'-O-ribose methyl-adenosine (i.e. 5'(m7Gp)(ppAm)[pN]n), it can be further methylated at the N6 methyl position to form N6-methyladenosine, resulting in 5'(m7Gp)(ppm6Am)[pN]n.

 

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12

When are mRNAs capped? How do the capping factors find the mRNAs?

Caps are put on pol II transcripts very soon after initiation. When the nascent pre-mRNA is about 25 nucleotides long, the process begins.

The capping enzymes ride along on the phosphorylated tail (CTD: carboxy terminal domain) of the RNA pol II large subunit. 

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13

endonuclease

Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain. Some, such as Deoxyribonuclease I, cut DNA relatively nonspecifically (without regard to sequence), while many, typically called restriction endonucleases or restriction enzymes, cleave only at very specific nucleotide sequences.

14

exonuclease

Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end (exo) of a polynucleotide chain. A hydrolyzing reaction that breaks phosphodiester bonds at either the 3’ or the 5’ end occurs.

Its close relative is the endonuclease, which cleaves phosphodiester bonds in the middle (endo) of a polynucleotide chain.

Eukaryotes and prokaryotes have three types of exonucleases involved in the normal turnover of mRNA: 5’ to 3’ exonuclease, which is a dependent decapping protein; 3’ to 5’ exonuclease, an independent protein; and poly(A)-specific 3’ to 5’ exonuclease

15

what type of nuclease would you use for decapping a mRNA (removing either a 5' cap or 3' poly(A) tail)?

Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end (exo) of a polynucleotide chain.

Eukaryotes and prokaryotes have three types of exonucleases involved in the normal turnover of mRNA: 5’ to 3’ exonuclease, which is a dependent decapping protein; 3’ to 5’ exonuclease, an independent protein; and poly(A)-specific 3’ to 5’ exonuclease.

16

Where is the poly(A) tail added?

To specific sequences near the 3’ end of the pre-mRNA that direct cleavage and polyadenylation 

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17

what does poly(A) stand for? basically what does it mean?

polyadenylation

the addition of many repeating adenine residues

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18

Where can poly(A) factors be found?

The polyadenylation factors ride along on the phosphorylated tail (CTD: carboxy terminal domain) of the RNA pol II large subunit.

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19

What are the roles of the cap and the poly(A) tail?

The cap protects the 5’ end from 5’ to 3’ exonucleases.
The poly(A) tail protects the 3’ end from 3’ to 5’ exonucleases.

Thus, both are important in regulating the half-life of the mRNA (eukaryotes need more time than prokaryotes, they must move the mRNA out of the nucleus)

20

What can happen to the poly(A) tail in the cytoplasm?

For some mRNAs, the poly(A) tail gradually shortens during its life in the cytoplasm. When it reaches a critical length, a de- capping enzyme is activated that removes the cap. The mRNA is then rapidly degraded in a 5’ to 3’ direction.

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21

How might an endonuclease shorten the life of a eukaryotic mRNA in cytoplasm?

For some mRNAs, the poly(A) tail gradually shortens during its life in the cytoplasm. The endonuclease may remove a portion of the AAA tail, causing the rest to shorten to the critical length much faster. When it reaches a critical length, a de- capping enzyme is activated that removes the cap. The mRNA is then rapidly degraded in a 5’ to 3’ direction

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22

What is the difference in stability between dsDNA, dsRNA, and DNA:RNA hybrids?

RNA:RNA > RNA:DNA > DNA:DNA

tl;dr: It's all about the helix! The addition of the 2' hydroxl does less to effect stability but sterically hinders the types of helix RNA can stabily adopt.

RNA is limited to B form, while DNA can adopt B, A or Z form.

RNA is more stable than DNA

(further reading)

One major difference between RNA and DNA is the detailed shape of the double-helix, A-form for RNA and predominantly B-form for DNA. RNA has never been observed to take on a B double-helix; the presence of the 2’-OH almost exclusively locks the ribose into a 3’-endo chair conformation, eliminating the possibility of a stable B-helix. However, the deoxyribose sugar may alternate between 2’-endo and 3’-endo conformations, allowing DNA to switch between B-form and A-form under the right circumstances. Note that hybrids of DNA:RNA (one strand of each in a double-helix) adopt an A-form conformation.

∆G = ∆H - ∆S(T)

In total, the single-strand to double-strand transition for both DNA and RNA is enthalpically (H) favors the helix and entropically (S) favors single-stranded conformation. For RNA, ∆H ~ 40 kJ mol-1/base pair and ∆S ~ 105 J K-1 mol-1/base pair (note the entropy is a function of temperature). For DNA, ∆H ~ 35 kJ mol-1/base pair and ∆S ~ 90 J K-1 mol-1/base pair. These are VERY large and OPPOSITE driving forces

In terms of the free energy, the balance of these interactions, we observed a higher melting temperature of RNA relative to the same sequence in DNA under normal conditions. The dominant source of this slightly higher energy for RNA is generally attributed to modestly better base-stacking energy in the A-form conformation. The precise nature of the molecular driving forces remain an active area of research. Experimentally, one observes very little difference in thermostability between RNA:DNA double-helix compared to an all RNA double-helix, consistent with the theory that the source of thermostability is due largely the result of A-form versus B-form conformational differences, not strictly differences in ribose versus deoxyribose chemistry.

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23

How did biologists first determine mRNA and genes are not 1:1 matches in eukaryotes?

By studing a gene from a human virus because:

1. human viral genes are expressed in human cells

2. the viral genome is simple to purify

3. the viral mRNAs are expressed at high levels and easy to purify

Purify DNA from Adenovirus virions, mRNA from infected cells.

Study by R-looping: hybridize DNA and mRNA. The DNA:RNA hybrid is stronger; the RNA will hybridize to the template strand of the DNA and displace the sense strand. Visualize using electron microscopy.

Results is mRNA is bound to complimentary areas (exons) and large loops exist in DNA where RNA is not bound (introns).

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intron

Introns are found in all types of RNAs (mRNA, rRNA, tRNA etc) in all types of organisms.

In splicing, the precursor RNA is made into a mature RNA and the intron is released.

No ATP needed for the splicing reaction because no new phosphodiester bonds are made.

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How does the cell know where to splice introns out of mRNA?

pre-mRNA introns have conserved sequences at both ends and in the center.

These are consensus sequences: most introns will not match the consensus sequences at all positions. Note that only a few bases are absolutely required.

The height of each base indicates its frequency in real introns. Note that only the first two bases and last two bases of the intron are absolutely required. One A in the branch site region is also necessary. 

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26

Why are certain conserved sequences reqired for intron splicing?

The conserved sequences participate directly in the splicing reaction, introns are removed in a two step reaction that involves all 3 conserved intron sequences. Changing these sequences changes the chemistry!!

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27

What are the two chemical steps in removing an intron from pre-mRNA?

The intron has a complex teritary structure (the linear RNA binds to itself and changes the location of its units in space, bringing some residues closer together which increases the potential for a reaction to occur and directs its pathway).

5'-(5' exon)-3' - 5'-(intron)-3' - 5'-(3' exon)-3' 

In the first step, the 2’ -OH of a conserved adenosine near the 3' end of the intron (branch site) attacks the phosphodiester bond of the 5' splice site (between the 5' exon and intron). This breaks the 3'-5' bond of between the 5' exon and the intron at the 5' splice site, and forms a new 2'-5' phosphodiester bond between the 3' end of the intron and the 5' end of the intron.

A conformal change occurs, placing the 3' - 5'phosphodiester bond of the conserved guanosine at the 3' end of the intron into position to be attacked by the recently-cleaved 3' -OH of the guanosine at the end the 5' exon.  This forms a new 3' - 5' phosphodiester bond between the 5' exon and the 3' exon (the 3' end of the 5' exon and the 5' end of the 3' exon ha!) and cleaves the intron loop, just as the 5' exon was cleaved in the first step.  Now the the intron is attatched to itself, in lariat form, and is degraded.

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Lariat

The "lasso" or rope form of the intron, as it is attatched to itself

 

A lasso (/ˈlæsoʊ/ or /læˈsuː/), also referred to as a lariat, riata, or reata (all from Spanish la reata), is a loop of ropedesigned as a restraint to be thrown around a target and tightened when pulled. It is a well-known tool of the American cowboy. The word is also a verb; to lasso is to throw the loop of rope around something. Although the tool has several proper names, such terms are rarely employed by those who actually use it; nearly all cowboys simply call it a "rope," and the use of such "roping." To most cowboys, the use of other terms — especially "lasso" — identifies the user as a layman.

29

What prevents you from splicing DNA with the same catalyst we use to splice introns from RNA?

In the reaction for removing a group II catalytic intron, one of the steps involves an attatck by 2' -OH of the conserved adenosine near the 5' end of the intron. DNA is 2-deoxyribo, because it doesn't have the 2' -OH, so it couldn't react in the same way as RNA and complete the splice.

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30

Besides substrate and product, what is the difference between a ribosome and a spliceosome?

The spliceosome (“splicing body”) is a large, multi-component machine made of RNAs and protein. It is named to highlight its similarity to the ribosome, another large machine made of RNA and protein. Unlike ribosomes, spliceosomes are assembled from pieces on each splicing substrate in a step-wise manner

31

What portions of the intron are conserved?

The height of each base indicates its frequency in real introns. Note that only the first two bases and last two bases of the intron and one A in the branch site region are absolutely required.

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How is spliceosome specificity achieved if only small portions at the splice sites are conserved?

Spliceosomes contain snRNPs (small nuclear ribonucleoprotein particles). snRNPs contain one snRNA (small nuclear RNA) and 7-15 proteins. They are stable and highly conserved across evolution.

They bind to splice sites and to each other to form a catalytically active spliceosome in an ordered, step-wise pathway. This combination of snRNPs allows for finer-grained recognition of different splice sites by different snRNPs on top of the conserved base that initates binding of the complex.

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Properties of snRNPs

Spliceosomes contain snRNPs (small nuclear ribonucleoprotein). snRNPs contain one snRNA (small nuclear RNA) and 7-15 proteins. Each snRNPs contains 7 proteins common to all snRNPs as well as several snRNP-specific proteins. They are stable and highly conserved across evolution.

They bind to splice sites and to each other to form a catalytically active spliceosome. 

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Properties of snRNAs

a. small

b. nuclear

c. stable

d. tri-methyl guanosine capped e. internally modified

f. U-rich

g. highly structured

h. highly conserved

i. abundant

They bind specific sets of proteins to make snRNP particles. Each snRNPs contains 7 proteins common to all snRNPs as well as several snRNP-specific proteins 

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U1 snRNP

Finds the 5' splice site of the intron

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U2 snRNP

Finds the branch site (BPS, branch point sequence) of the intron, guided by pre-assembled proteins (BBP, U2AF) that recognise the branch site before snRNPs arrive

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U6 snRNP

Likely the 'catalytic snRNA' (not a typo, snRNPs contain snRNAs that catalyse the reaction. The proteins are there for regulation and to assist in aligning the snRNAs). Generally tightly complexed with snRNP U4, again likely to keep U6 deactivated until reaching an accurately-formed spliceosome.

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Group II intron vs. Group I intron vs. tRNA intron

Group II introns are closely related to the pre-mRNA introns we have been discussing. They use the identical two step mechanism and even have sequence similarities.

Group I introns are spliced using a similar chemistry, but the recognition steps are very different.

tRNA introns are completely different: they are removed by protein enzymes that cut the pre-tRNA and paste it together.

39

Spliceosome catalysed splicing of nuclear mRNA is chemically most similar to the reaction in what type of self-splicing intron?

Group II introns

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40

U2AF

U2AF “U2 auxiliary factor” is a non-snRNP protein important for splicing, binds the region between the branch site and the 3’ splice site. It is required for splicing and acts by helping U2 find branch site. It is a heterodimer of two proteins. The large protein has several domains common to RNA binding proteins:

3 “RNA recognition motifs” or RRMs
1 domain rich in arginine and serine called a RS domain 

41

BBP

BBP is ‘branchpoint binding protein’, it binds the branch site (BPS, branch point sequence) before U2. It is not part of any snRNP. 

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hnRNP

hnRNP proteins are “heterogenous nuclear ribonucleoproteins”. They are like histones for RNA.

43

How could snRNPs be recruited to the exon?

Many proteins probably bind both introns and exons in the pre-mRNA and interact with the snRNPs.

A large family of SR proteins (which contain the RS domain, rich in arginine and serine) bind to ESEs (Exonic Splicing Enhancers) in the exons. SR proteins help recruit U1 snRNP and other splicing factors. Some SR proteins can repress splicing.

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44

What is the overall order of binding of protein to the mRNA before splicing?

Binding by SR proteins and others followed by BBP, U2AF, and then by snRNPs U1 and U2, other site specific snRNPs, then finally U4/U5/U6.

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How is the proteome bigger than the genome?

With alternative splicing, some mRNAs are spliced differently in different tissues and at different times.

There are many different types of alternative splicing, giving rise to protein isoforms: the proteins generated by alternative splicing are not wildly different from one another, just slightly different.
This allows modulation of protein function.
Alternative splicing uses variations of constitutive splicing mechanism 

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Constitutive exon in alternative splicing

In alternative splicing, constitutive exons are present in every transcript

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Mutually exclusive exon in alternative splicing

Mutually exclusive exons: One of two exons is retained in mRNAs after splicing, but not both.

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Cassette exon in alternative splicing

Exon skipping or cassette exon: in this case, an exon may be spliced out of the primary transcript or retained. This is the most common mode in mammalian pre-mRNAs.

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Alternative donor site in alternative splicing

Alternative donor site: An alternative 5' splice junction (donor site) is used, changing the 3' boundary of the upstream exon.

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Alternative acceptor site in alternative splicing

Alternative acceptor site: An alternative 3' splice junction (acceptor site) is used, changing the 5' boundary of the downstream exon.

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Intron retention in alternative splicing

Intron retention: A sequence may be spliced out as an intron or simply retained. This is distinguished from exon skipping because the retained sequence is not flanked by introns. If the retained intron is in the coding region, the intron must encode amino acids in frame with the neighboring exons, or a stop codon or a shift in the reading frame will cause the protein to be non-functional. This is the rarest mode in mammals.

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52

Aside from selective exon incorporation in the final mRNA product, how else might one gene code for more than one mRNA?

In addition to these primary modes of alternative splicing, there are two other main mechanisms by which different mRNAs may be generated from the same gene; multiple promoters and multiple polyadenylation sites. Use of multiple promoters is properly described as a transcriptional regulation mechanism rather than alternative splicing; by starting transcription at different points, transcripts with different 5'-most exons can be generated. At the other end, multiple polyadenylation sites provide different 3' end points for the transcript. Both of these mechanisms are found in combination with alternative splicing and provide additional variety in mRNAs derived from a gene.

53

Without changing exons, poly(A) tail addition locus, or RNA transcription start site, what is an additional way for mRNAs to be edited?

The addition, removal, translation or transversion of bases not in the original DNA code, after transcription. In some cases, different nucleotides such as inosine (I) are incorporated

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54

inosine

Inosine is a nucleoside that is formed when hypoxanthine is attached to a ribose ring (also known as a ribofuranose) via a β-N9-glycosidic bond.

Inosine is commonly found in tRNAs and is essential for proper translation of the genetic code in wobble base pairs.

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wobble base pair

A wobble base pair is a pairing between two nucleotides in RNA molecules that does not follow Watson-Crick base pairrules. The four main wobble base pairs are guanine-uracil (G-U), hypoxanthine-uracil (I-U), hypoxanthine-adenine (I-A), and hypoxanthine-cytosine (I-C). In order to maintain consistency of nucleic acid nomenclature, "I" is used for hypoxanthine because hypoxanthine is the nucleobase of inosine; nomenclature otherwise follows the names of nucleobases and their corresponding nucleosides (e.g., "G" for both guanine and guanosine). The thermodynamic stability of a wobble base pair is comparable to that of a Watson-Crick base pair. Wobble base pairs are fundamental in RNA secondary structure and are critical for the proper translation of the genetic code.