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Microbiology > Exam 3 > Flashcards

Exam 3 Flashcards

(49 cards)

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1
Q

Fred Griffith infected mice with what?

A

Streptococcus pneumoniae

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2
Q

What was the result of the experiment by Griffith in terms of strain combination?

A

Dead S + Live R = Kills mice

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3
Q

conclusion of the Griffith experiment

A

Living R has been TRANSFORMED to living

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4
Q

Is a mouse essential to the Griffith experiment?

A

No, it can be done in a dish

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5
Q

What did the Transformation experiment by Griffith set the stage for?

A

Fractionation experiments

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6
Q

what was the conclusion of the experiment by Avery, Macleod, and McCarty?

A

They concluded that DNA was hereditary material instead of protein

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7
Q

R cells + purified S cell polysaccharide ->

A

R colonies

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8
Q

R cells + purified s cel protein ->

A

R colonies

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9
Q

R cells + purified S cell RNA ->

A

R colonies

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10
Q

R cells + purified S cell DNA ->

A

S colonies

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11
Q

S cell extract + protease + R cells ->

A

S colonies

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12
Q

S cell extract + RNase + R cells ->

A

S colonies

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13
Q

Objection to Avery experiment by Harriet Taylor showed what result?

A

R DNA transforms ER to R; Exposure of these R to S DNA transforms to S; and S DNA transforms Er to S

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14
Q

What did Hotchkiss do?

A

Isolated penicillin resistant and streptomycin resistant S. Pneumoniae

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15
Q

In the Hotchkiss experiment, DNA from both the penicillin resistant and the streptomycin resistant strands can be used to transform what type of strains?

A

Sensitive

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16
Q

Conclusion of the Hershey and Chase experiement

A

DNA had the genetic info for synthesis of both viral components (coat protein with 32P DNA and 35S protein in coat)

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17
Q

Exogenous

A

DNA that is transferred to recipient

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18
Q

Endogenote

A

Genome of recipient

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19
Q

Merozygote

A

Recipient cell that is partially diploid as result of transfer process

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20
Q

Which experiments did Lederberg and Tatum do?

Study These Flashcards
A

Recombination

21
Q

What is the difference between conjugation and transduction?

Study These Flashcards
A

Conjugation has the pilus that connected F+ and F-

22
Q

Hfr donor strands are the high frequency donor of only limited potion of the _____ gene

23
Q

What is tra for Hfr

Study These Flashcards
A

Transfer function of f factor

24
Q

Hfr mapping: Gene C (Hfr 1) donated first in clockwise/counterclockwise) order?

25
Hfr mapping: Gene L (Hfr 2)donated first in clockwise/counterclockwise) order?
Counterclockwise
26
Hfr mapping: Gene X (Hfr 3) donated first in clockwise/counterclockwise) order?
Clockwise
27
Hfr mapping: Gene G (Hfr 4) donated first in clockwise/counterclockwise) order?
Counterclockwise
28
what is the outcome of Hfr mapping?
Be able to map the genes across chromosomes
29
Transduction is the transfer of bacterial genes via what/
virus
30
difference between generalized and specialized transduction?
Generalized is the lytic cycle ad specialized is the lysogenic phase
31
Where is cilia located?
On the epiplasm membrane
32
What are the parts of cilia?
- 9 outer tubules - 1 complete, 1 incomplete - 2 core tubules - Dyenin arms
33
What do the Dynein arms of cilia do?
- motor proteins of cilia - creates a current of some laminar and some turbulent flow - walks in the (-) minus direction
34
What is cAMP?
- Cyclic Adenosine Monophosphate - signaling molecule for MANY organisms
35
What is the main output of the RuBisCO and Calvin pathway?
End generation of sugar (G3P)
36
Virulence
Replication, synthesis, assembly, and lysis
37
lysogeny
For lambda, NA is inserted into att sites n the bacterial chromosome. DNA is then replicated as the bacteria replicated
38
If c1 > Cro ->
Lysogeny
39
If Cro > c1 ->
Lytic
40
DsDNA ->
Herpes
41
SsDNA ->
Phage MB
42
DsRNA ->
Reoviridae
43
(+) stranded RNA ->
Coronavirus
44
(-) stranded RNA ->
E. Bola
45
viral lifestyle
1. Attachment 2. Entry 3. synthesis 4. Assembly 5. Release
46
HSV stands for what? Two types?
Herpes Simplex Virus - HV 1 = cold sores - HV 2 = genital herpes
47
In (=) strand RNA virus, HIV, what is gp41 and gp120?
Gp41 = transmembrane Gp120 = docking
48
Retrovirus
RNA virus that is replicated in a cell using the enzyme “reverse transcriptase”
49
HAART
Highly active anti-retro viral therapy

Microbiology (11 decks)

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