Exam 3

Question 1

components of a lac o

Question 2

how does mRNA leader regulate tryptophan production

Question 3

eukaryotic gene expression

Question 4

prokaryotic gene expression

Question 5

levels of eukaryotic gene control

Question 6

transcription factors

Question 7

how do transcription factors initiate eukaryotic transcription

Question 8

how do oncogenes trigger cancer

Question 9

describe the defective cell control mechanisms in cancer

Question 10

describe the actions of tumor suppressor genes and the p53 genes

Question 11

define transcription factors and their role in initiating eukaryotic transcription

Question 12

HIV is a retrovirus. In order to prevent incorporation of the viral DNA into the host genome, antiretroviral drugs could target ____

Question 13

what are the features of genetic code

Question 14

the central dogma of retroviruses:

Question 15

within a eukaryotic genome, an enhancer may be located

Question 16

what are the various steps in eukaryotic gene regulation

Question 17

describe the lac operon components and its regulation in prokaryotes

Question 18

genes that are always “turned on” in a cell are referred to as ____ genes

Question 19

genes associated with embryonic development are known as ____

Question 20

Regulation where expression only occurs when levels of a chemical are low

Question 21

Pathway that is subject to end product repression

Question 22

sigma factors can regulate the ___ transcription

Question 23

effector molecules

Question 24

a transcriptional regulator that decreases transcription of a gene from a vast distance in known as

Question 25

enhancer

Question 26

regulator

Question 27

upstream control element

Question 28

silencer

Question 29

describe cAMP/Crp regulation of the lac operon

Question 30

define eukaryotic gene control features

Question 31

what are the steps in cloning a human insulin gene

Question 32

what are the functions of restriction enzymes and DNA ligase

Question 33

how are cDNA and Genomic libraries prepared

Question 34

describe the events in genetic engineering of plant cells using Ti plasmids

Question 35

what is gel electrophoresis

Question 36

what is DNA fingerprinting

Question 37

what is a cloning vector

Question 38

how are transgenic animals created

Question 39

BACs

Question 40

YACs

Question 41

Cosmids

Question 42

what is bioinformatics

Question 43

what is PCR and its applications

Question 44

describe DNA cloning techniques for production of human proteins in a bacterium

Question 45

southern blot

Question 46

northern blot

Question 47

western blot

Question 48

describe how PCR and DNA sequencing is done

Question 49

describe some uses of restriction fragment length polymorphisms

Question 50

role of G1

Question 51

role of G2

Question 52

role of spindle assembly

Question 53

role of M checkpoints during interphase

Question 54

prophase

Question 55

prometaphase

Question 56

metaphase

Question 57

anaphase

Question 58

telophase

Question 59

how does cytokinesis differ between plant and animal cells