exp 3.7 use starch agar yo show digestive activity during gemination Flashcards

1
Q

what is the nutrient

A

starch

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2
Q

what is the medium

A

agar

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3
Q

what agar is used

A

starch agar

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4
Q

what seeds are used

A

broad bean seeds

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5
Q

why are the seeds soaked in water

A

to soften the testa

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6
Q

what is the control

A

boiled seeds

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7
Q

how are seeds placed in petri dish

A

cut in half, face down

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8
Q

where is the petri dish left?

A

incubator at 20-30 degrees

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9
Q

how long are seeds left in incubator

A

2 days

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10
Q

why is the incubator at 20-30 degrees

A

optimum temperature for enzyme activity

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11
Q

aspetic techniques used

A

seeds soaked in disinfectant
petri dish opened minimally for a short period of time

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12
Q

sterile techniques used

A

forceps are flamed

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13
Q

what is used to test for the presence of starch

A

iodine

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14
Q

what happens when iodine is added to the petri dish

A

the plate will turn blue/black except for where the seeds were placed which will remain yellow proving that germination has occurred

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15
Q

what happens when iodine is added to a plate with boiled seeds

A

whole plate turns blue/black

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16
Q

name the enzyme, substrate and product

A

starch = substrate
amylase = enzyme
maltose = product