EXPERIEMT #14 FINALS

Question 1

What are we talking about in experiment 14?

Answer 1

Albumin
Total serum proteins
A/G ratio

Question 2

How are you going to correlate test results with pathologic conditions?

Answer 2

Electrophoresis pattern

Question 3

What are you going to explain in Experiment #14?

Answer 3

Amino acid synthesis and metabolism

Question 4

What are you going to discuss in Experiment #14?

Answer 4

The different types of aminoacidopathies

Question 5

In Serum Albumin Determination, what method is used?

Answer 5

Bromocresol Green Method (BCG)

Question 6

What is the principle of Serum Albumin Determination?

Answer 6

The binding behavior of the protein with an anionic dye.

Question 7

Principle of Serum Albumin Determination:

Determination of __________ in ____________is usually based on the ___________________, _____________ forming a ____________.

Answer 7

Albumin
serum or plasma
binding behavior of the protein with an anionic dye
bromcresol green
green colored complex.

Question 8

What is the concentration standard of Total protein?

Answer 8

6g/dL

Question 9

What is the concentration standard of Serum Albumin?

Answer 9

4g/dL

Question 10

What is the formula for computing Total protein and serum standard?

Answer 10

Cu= Au/Astd x Cstd

Question 11

How much serum albumin reagent is added?

Answer 11

3.0mL

Question 12

To which tube is serum albumin reagent added?

Answer 12

Blank
Standard
Sample

Question 13

How much protein standard is added?

Answer 13

3.0mL

Question 14

To which tube protein reagent is added?

Answer 14

Blank
Standard
Sample

Question 15

How much and to which tube water is added for both serum and Albumin determination?

Answer 15

20uL
Blank

Question 16

How much and to which tube STANDARD is added for both serum albumin and total protein determination?

Answer 16

20uL
Standard sample

Question 17

How much and to which tube SERUM is added for both serum Albumin and total protein determination?

Answer 17

20uL
Sample

Question 18

In performing Albumin, total protein determination, what specimen is used?

Answer 18

Serum

Question 19

In SAD and Total Protein, at what temperature are we going to stand the samples?

Answer 19

Room temperature

Question 20

Under SAD, at what absorbance are we going to read the blank, standard and sample?

Answer 20

600nm

Question 21

Under SAD, how much time is its stability?

Answer 21

60 MINUTES / 1 HR

Question 22

In determination of Total Proteins, what method is used?

Answer 22

Biuret (Modified)

Question 23

What is the principle of Biuret Method?

Answer 23

The copper binds to the peptide bond structure of the protein.

Question 24

When a solution containing proteins is rejected with a tartrate-complex cupric ions and
alkali, the copper binds to the peptide bond structure of the proteins. What is the color that is produced?

Answer 24

Purple colored chromogen

Question 25

When a solution containing _________ is _________with a ______________ and ________, the copper binds to the peptide bond structure of the proteins, forming a purple colored chromogen.

Answer 25

proteins rejected tartrate-complex cupric ions alkali

Question 26

Under SAD, stand the samples at room temperature for?

Answer 26

1 minute

Question 27

Under Total Protein, stand the samples at room temperature for?

Answer 27

5 minutes

Question 28

TRUE OR FLASE: Inverting the tubes is necessary in performing SAD and Total Albumin determination.

Answer 28

TRUE

Question 29

What is the formula in determination of Globulin?

Answer 29

Globulin = gms. of total protein - gms. of albumin

Question 30

What is the formula in determination of A/G Ratio?

Answer 30

A/G ratio = gms, of Albumin / gms. of Globulin

Question 31

What do you call the procedure used in SAD and Total Albumin determination?

Answer 31

Colorimetric Procedure

Question 32

What is the normal value of TOTAL PROTEIN in Conventional and S.I unit?

Answer 32

6 - 8 60 - 80

Question 33

What is the normal value of ALBUMIN in Conventional and S.I unit?

Answer 33

3.5 - 5 35 - 50

Question 34

What is the normal value of GLOBULIN in Conventional and S.I unit?

Answer 34

1.8 - 3.2 18 - 32

Question 35

What is the normal value of A/G RATIO in Conventional?

Answer 35

1.5-2.4 : 1

Question 36

What is the color of the complex formed when albumin in serum or plasma binds with the anionic dye, bromcresol green, during the determination of albumin?

Answer 36

Green colored complex