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Biochem Lab Midterm > Experiment 4 > Flashcards

Experiment 4 Flashcards

(56 cards)

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1
Q

Is the unfolding of complex secondary tertiary and quaternary structure of proteins

A

Denaturation

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2
Q

Is the unfolding of native proteins into randomly called biologically inactive form

A

Denaturation

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3
Q

Denaturation is a _______ process

A

Reversible

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4
Q

Unfolded molecule will ____ to its native form when the condition becomes _____

A

Return, favorable

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5
Q

Other name for renaturation

A

Refolding
Annealing

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6
Q

Renders the protein insoluble at isoelectric point causing it to precipitate

A

Flocculation

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7
Q

Is a simple clamping together of dispersed chains of denatured protein

A

Flocculation

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8
Q

The clam change become matte together in a mass which is insoluble

A

Coagulation

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9
Q

What are the denaturing agents

A

Sodium dodecyl sulfate
Urea Guanadium hydrochloride
B-mercaptoethanol

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10
Q

Is a denaturing agents which disrupts hydrophobic interaction and H bonding

A

Sodium dodecyl sulfate

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11
Q

A denaturing agent that disrupts hydrophobic and electrostatic interaction and H bonding

A

Urea guanidium hydrochloride

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12
Q

A denaturing agent that disrupts hydrophobic interactions if detergent is charged it can disrupt electrostatic interaction

A

B-mercaptoethanol

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13
Q

Denaturing agents that disrupts disulfide bridges and electrostatic interactions

A

Heavy metal ions

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14
Q

A denaturing agents that disrupt H bonding

A

Alcohol

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15
Q

Denaturing agent that disrupt H bonding and electrostatic interaction

A

Acid or bases

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16
Q

A denaturing agent that combines with positively charged proteins and disrupt electrostatic interaction

A

Alkaloidal reagent

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17
Q

What are the denaturing processes

A

Heat
Mechanical disruption
Drastic pH change

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18
Q

In precipitation reaction what is the result of concentrated sulfuric acid H2SO4

A

No formation of precipitate

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19
Q

In precipitation reaction what is the result of concentrated hydrochloric acid HCl

A

Formation of white precipitate

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20
Q

In precipitation reaction what is the result of concentrated nitric acid HNO3

A

Formation of yellow precipitate

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21
Q

In precipitation reaction what is the result of potassium hydroxide and glacial HAc

A

Colorless

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22
Q

In precipitation reaction what is the result of heller’s test

A

Yellow ring, due to the nitrition of benzene ring of the amino acid present in the protein albumin.

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23
Q

In precipitation reaction what is the result of robert’s test

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A

White ring or white precipitate

24
Q

In precipitation by metallic salts what is the result of silver nitrate AgNO3

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A

White precipitate

25
In precipitation by metallic salts what is the result of copper sulfate CuSO4
Bluish white precipitate
26
In precipitation by metallic salt what is the result of ferric chloride FeCl3
yellow precipitate
27
In precipitation by metallic salt what is the result of barium chloride BaCl2
White precipitate
28
In precipitation by alkaloidal agents what is the result of picric acid
Yellow precipitate
29
In precipitation by alkaloidal reagents what is the result of trichloroacetic acid
White precipitate
30
In precipitation by alkaloidal reagents what is the result of tannic acid
Brown precipitate
31
In precipitation by alkaloidal reagents what is the result of phosphotungstic acid
White precipitate
32
In a precipitation by alkaloidal reagents what is the result of phoshomolybdic acid
Yellowish white precipitate
33
In precipitation by alcohol what is the result of alcohol HCl and egg albumin
Formation of clear solution
34
In precipitation by alcohol what is the result of alcohol, NaOH and egg albumin
Turbid or cloudy solution
35
In precipitation by alcohol what is the result of alcohol and egg albumin
White precipitate
36
In salting out what is the result of ammonium sulfate
Blue solution
37
In salting out what is the result of sodium chloride
Purple solution
38
In denaturation and coagulation of egg albumin what is the result of albumin and hydrochloric acid
Turbid solution with white precipitate
39
In denaturation and coagulation of egg albumin what is the result of albumin and acetate buffer solution
Clear solution with formation of gel like white precipitate
40
In denaturation and coagulation of egg albumin what is the result of albumin and sodium hydroxide
No apparent changes
41
In color reaction test what is the result of milllons test
Flash the red precipitate and a presence of tyrosine
42
In color reaction test what is the result of xanthropotic test
Yellow to orange color and a presence of tyrosine tryptophan or phenylalanine
43
In color reaction test what is the result of biuret test
A purple color and presence of peptide bonds
44
In color reaction test what is the result of hopkins cole test
A violet ring and a presence of tryptophan
45
The ______ are used as antiseptic and germicides by precipitating bacterial protein
Metallic salt
46
The use of ______ for cauterization of wants and ulcer depends upon its property of precipitating tissues protein
Silver nitrate
47
In ________ antidotes like _____ and ____ are given which form insoluble precipitate which can be removed subsequently by means of stomach tube
Metallic poisoning, egg white, milk
48
Has less germicidal property because it readily coagulates proteins and the coagulum forms a protective coating on the surface of the bacteria
Pure alcohol (95%)
49
How many percent of alcohol has a greater germicidal
70%
50
A decrease in the quantity of proteins in the blood
Hypoproteinemia
51
The presence of protein in the urine this may indicate the presence of damage to or disease on the kidneys
Proteinuria
52
The presence of serum albumin serum globulin or other serum proteins in the urine
Albuminuria
53
Causes the atoms within the protein molecules to vibrate more rapidly causing the hydrogen bonds and hydrophobic interactions to break
Heat
54
Split salt linkages by ionizing the carboxylic group
strong acids
55
Denature the protein by disrupting the hydrogen bonds
Alcohol
56
Unfolded molecule will return to its native form when the conditions becomes favorable
Renaturation

Biochem Lab Midterm (5 decks)

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