Experiments - Rate Of Transpiration Flashcards
(11 cards)
How could you plan an experiment to see the impact of temperature on the respiration of yeast
Resp = enzyme controlled
Produces co2
1) place yeast in a sugar solution
2) water bath at 20C for 5 minutes
3) measure volume of co2 guven off in 2 minutes with a gas syringe
4) Repeat twice more
5) Repeat all steps at 5 dif temperatures
What equipment is needed in the experiment to investigate light as a limiting factor of photosynthesis
Beaker
Sodium hydrogen carbonate solution
Test tube
Funnel
Stopwatch
Lamp
Meter ruler
Pondweed
Method for experiment investigating light as the limiting factor of photosynthesis
1) Place 500ml of sodium hydrogen carbonate solution (co2 for photosynthesis) in a beaker with a piece of pondweed
2) put the wide end of the funnel over the pondweed
3) submerge the pondweed and funnel under the solution
4) Fill a test tube with the remaining solution and and put your thumb over the opening to ensure no air bubbles get in
5) turn the test tube upside down and place it over the small opening of the funnel
6) position the lamp 10cm away from the beaker
7) leave for 2 minutes and count the no of co2 bubbles produced
8) repeat twice more and take mean discounting anomalies
9) repeat step 7 with dif distances
Apparatus and equipment needed for investigating the rate of transpiration with a potometer experiment
Bubble potometer- gas tube, ruler, reservoir
Spray bottle
Stopwatch
Hairdryer
Leafy shoot
Method for transpiration and potometer experiment
Submerge the potometer underwater to fill it
Cut the leafy shoot at an angle underwater
Fit the leafy shoot into the rubber bung
Take the potometer out of the water and smear the junctions with Vaseline to keep out air
Dry off the leaves of the leafy shoot
Introduce an air bubbles by lifting up the bubble potometer slightly out of the beaker
Record the position of the bubble when it reaches the ruler
Start a two minute timer and record the position of the bubble every 30 seconds
Calculate the distance moved by the bubble
Repeat twice
Repeat para 2 whilst changing factors impacting rate of transpiration eg outside for temp, hairdryer for wind and heat, spray bottle for humidity
VIDEO
What would you change to investigate the rate of enzyme controlled reactions and how?
Temperature - water bath
PH - dif solutions
What are the factors affecting the rate of respiration
Temperature
Glucose concentration
Oxygen concentration
How does temperature affect the rate of respiration?
Enzyme activity graph - low = no kinetic for collisions
High = denatured
How does glucose concentration impact the rate of respiration?
More glucose increases respiration if it is the limiting factor
How does oxygen impact the rate of respiration
More energy released with aerobic than anaerobic as more bonds broken
How to see production of carbon dioxide
Bubble gas through
Lime water should turn cloudy
