Exploring genes and genomes Flashcards Preview

BB1702 Biochemistry: Structure and Function > Exploring genes and genomes > Flashcards

Flashcards in Exploring genes and genomes Deck (4)
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What are some of the key techniques used in exploring the genome?

  • Restriction-enzyme analysis
  • Blotting techniques
  • DNA sequencing
  • Solid-phase synthesis of nucleic acids
  • Polymerase chain reaction (PCR)


What are restriction enzymes and how can they be used by scientists?

Enzymes that recognise specific base sequences in double-helical DNA and cleave, at specific places, both strand of that duplex.

Scientists can exploit this and cleave DNA into specified fragments that can be analysed.


Restricton enzymes cleave what kind of sequences?

Palindromic sequences