Fermenters

Question 1

Media that is used to detect fermentation/acid production with Bromthymol blue as indicator

Answer 1

OF Media of Hugh and Leifson

Question 2

Media that is used to detect fermentation/acid production with Phenol red as indicator

Answer 2

OF Media - CDC method

Question 3

How many tubes are used in OF Media of Hugh and Leifson?

Answer 3

Two (2) tubes

Open (aerobic) and closed (anaerobic)

Question 4

OF Media of Hugh and Leifson:

1. Positive result:
2. Negative result:

a. Yellow
b. Blue green/Green

Answer 4

1. Positive result – a. Yellow
2. Negative result – b. Blue green/Green

Question 5

Interpretation of result: OF Media of Hugh and Leifson

Open/Closed

1. +/+
2. +/-
3. -/-

a. Oxidizer/Non-fermenters
b. Non-oxidizers/Non-saccharolytic
c. Fermenter

Answer 5

1. +/+ = Fermenter
2. +/- = Oxidizer/Non-fermenters
3. -/- = Non-oxidizers/Non-saccharolytic

Question 6

How is Triple Sugar Iron dispensed?

Answer 6

As slant & butt

Question 7

Oxygen requirement of Enterobacteriaceae

Answer 7

Facultative anaerobe

Question 8

All Enterobacteriaceae are non-fermenters.

a. True
b. False

Answer 8

b. False.

All are fermenters.

Question 9

Usual media to detect Enterobacteriaceae’s ability to produce acid (ferment)

Answer 9

Triple Sugar Iron

Question 10

What are the three (3) fermentable carbohydrates in TSI and their corresponding measurement?

Answer 10

1. Glucose (1 part)
2. Lactose (10 parts)
3. Sucrose (10 parts)

Question 11

TSI media can also be used as H2S indicators.

a. True
b. False

Answer 11

a. True

Question 12

How can TSI show H2S production?

Answer 12

TSI has two (2) H2S indicators: Ferrous sulfate and sodium thiosulfate

Question 13

Match: TSI

1. Acid pH
2. Alkaline pH

a. K
b. A

Slant/Butt color:

• Yellow
• Red

Answer 13

1. Acid pH - A - Yellow
2. Alkaline pH - K - Red

Question 14

Manner of reporting of TSI result

Answer 14

Slant/Butt (Slant over butt)

Question 15

Match the results:

1. K/K
2. K/A
3. A/A

a. Non-lactose fermenter
b. Lactose fermenter
c. Non fermenter

Slant/Butt Color:

• Yellow
• Red

Answer 15

1. K/K - Non fermenter - Red/Red
2. K/A - Non-lactose fermenter - Red/Yellow
3. A/A - Lactose fermenter - Yellow/Yellow

Question 16

E.coli, Enterobacter, Klebsiella are what type of fermenters?

Answer 16

Lactose fermenters

Question 17

Shigella spp., Proteus, Providencia and Morganella are what type of fermenters?

Answer 17

Non-lactose fermenters

Question 18

Citrobacter, Y. enterocolitica, and S. sonnei are what type of fermenters?

Answer 18

Late lactose fermenters

Question 19

P. aeruginosa is what kind of fermenter?

Answer 19

Non-fermenter

Question 20

Two possible results of Enterobacteriaceae in TSI

Answer 20

1. K/A
2. A/A

Question 21

Four (4) tests that will differentiate Salmonella-Shigella

Answer 21

1. Motility test
2. H2S production
3. Malonate
4. Decarboxylase test

Question 22

Salmonella or Shigella:

• Motile, H2S (+), Malonate and Decarboxylase (+)

Answer 22

Salmonella

Question 23

Salmonella or Shigella:

Nonmotile, H2S (-), Malonate and Decarboxylase (-)

Answer 23

Shigella

Question 24

Salmonella and Shigella are what kind of pathogens?

a. Respiratory
b. Oral
c. Genital
d. Intestinal

Answer 24

d. Intestinal pathogens

Both are agents of diarrheal disease

Question 25

Salmonella and shigella are never normal flora. a. True b. False

Answer 25

a. True

Question 26

These characteristics will separates Vibrio spp. from the Genus Aeromonas.

Answer 26

Vibrio spp. - String test (+); Susceptible to Vibriostatic Agent Aeromonas - String test (-); Resistant to Vibriostatic Agent

Question 27

Vibriostatic agent is also known as

Answer 27

0/129 agent

Question 28

Selective differential media for Vibrios

Answer 28

Thiosulfate Citrate Bile salt Media (TCBS)

Question 29

pH Indicator of TCBS

Answer 29

Thymol blue Bromthymol blue

Question 30

What is the carbohydrate present in TCBS?

Answer 30

Sucrose

Question 31

Color of colonies that are sucrose fermenters

Answer 31

Yellow

Question 32

Color of colonies that are non-sucrose fermenters

Answer 32

Green

Question 33

Enrichment media for Vibrios

Answer 33

Alkaline Peptone Water

Question 34

Vibrio spp. that are non-sucrose fermenters

Answer 34

V. mimicus, V. parahaemolyticus, V. vulnificus (MPV)

Question 35

Vibrio spp. that are sucrose fermenters

Answer 35

V. alginolyticus V. cholerae, V. fluvialis V. furnissi V. metschnikovii (ACFFM)

Question 36

All Vibrios are halophilic, except:

Answer 36

1. V. cholerae 2. V. mimicus Both are non-halophilic

Question 37

How much salt concentration is required for Vibrios?

Answer 37

8-10%

Question 38

Other term for Beta-hemolysis due to TDH

Answer 38

Kanagawa phenomenon

Question 39

Media used to demonstrate Kanagawa phenomenon of V. parahaemolyticus

Answer 39

Wagatsuma agar

Question 40

Component of Wagatsuma agar

Answer 40

High salt mannitol concentration

Question 41

Causative agent of Summer Diarrhea in Japan

Answer 41

V. parahaemolyticus

Question 42

Causative agent of Rice watery stool

Answer 42

V. cholerae

Question 43

Cholera is what kind of disease, and characterized by?

Answer 43

Diarrheal disease characterized by Rice watery stool

Question 44

What is TDH?

Answer 44

Thermostable Direct Hemolysis

Question 45

Characteristic of Vibrio that will separate them from Enterobacteriaceae

Answer 45

Vibrio - Oxidase (+) Enterobacteriaceae - Oxidase (-)

Question 46

Characteristic of Vibrio that will separate them from Plesiomonas

Answer 46

Vibrio - Inositol (-) Plesiomonas - Inositol (+)

Question 47

Characteristic of Vibrio that will differentiate them from Pseudomonas

Answer 47

Vibrio - Fermenters (all glucose fermenters; lactose (-) except V. vulnificus Pseudomonas - Non-fermenters

Question 48

Vibrio spp. that is less virulent, occupational hazard → continuous contact with sea water (fishermen, sailor)

Answer 48

V. alginolyticus

Question 49

Most significant vibrio

Answer 49

V. cholerae

Question 50

Species that is Triple Decarboxylase negative (-)

Answer 50

Pantoea agglomerans

Question 51

Pantoea agglomerans is negative to which amino acids?

Answer 51

Negative to Lysine, Ornithine and Arginine

Question 52

Species positive in Triple Decarboxylase

Answer 52

Pleisomonas spp.

Question 53

This test is done to detect if the organism produces decarboxylase enzyme

Answer 53

Decarboxylase test

Question 54

What are the two (2) ways to do Decarboxylase test?

Answer 54

1. Moeller's broth 2. LIA

Question 55

Carbohydrate and indicator present in Moeller's broth

Answer 55

Glucose Bromcresol purple

Question 56

Color result in Decarboxylase test

Answer 56

(+) purple (-) yellow

Question 57

What does decarboxylase enzyme do in decarboxylase test?

Answer 57

Decarboxylase enzyme removes carboxyl group from the amino acid

Question 58

Match: Decarboxylase Test Amino acids 1. Lysine 2. Ornithine 3. Arginine Carboxyl Group a. Putrescine b. Citrulline c. Cadaverine

Answer 58

1. Lysine - Cadaverine 2. Ornithine - Putrescine 3. Arginine - Citrulline

Question 59

Campylobacter spp. that appears S-shaped or wings of seagulls

Answer 59

C. jejuni and C. coli

Question 60

Motility of C. jejuni and C. coli

Answer 60

Darting motility

Question 61

Growth requirement of Campylobacter jejuni and Campylobacter coli

Answer 61

1. Capnophilic 2. Microaerophilic

Question 62

The virulence factor of which organism is production of urease?

Answer 62

Helicobacter pylori

Question 63

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Answer 63

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