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Flashcards in Final Deck (16)
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1

Egg yolk agar

Detects Lipase, Lecithinase, and protease activities

2

Which Clostridium spp has a double zone of hemolysis?

C. Perfringens

3

Skim milk plates

Detects proteolysis of caesin protein by caseinase enzyme

4

Spirit Blue Plates

Contains lipids and an indicator of pH for detection of lipase

5

Nutrient Gelatin Tubes

Detects gelatin liquidification (gelatin protease activity)

6

1% Tryptone broth

Used for detection of indole production by tryptophanase

7

Litmus Milk Tubes

Provides lactose as a sugar source, Casein as a protein source, and looks for pH change

8

McFarland Turbidity Standard

0.5 standard that contains 99.5 ml of 1% sulfuric acid and 0.5 ml 1.175% barium chloride and is used to dilute an inoculum until its turbidity matches the sample

9

Mueller-Hinton Agar

Special agar for testing antimicrobial susceptability

10

Minimum Inhibitory Concentration

A tube dilution method of measuring antimicrobial susceptibility. Measured by the lowest concentration of a drug that inhibits growth

11

Minimal Bactericidal Concentration

The least concentration of an antimicrobial that is needed to kill 99.9% of organisms

12

Potato Dextrose Agar

A high sugar growth medium used to grow fungi with a slightly acidic pH to inhibit bacterial growth

13

10% KOH

Used in dermal isolates to dissolve keratinized cells leaving chitinous cell walls of fungi. Hyphae can be observed microscopically

14

Lactophenol cotton blue

Stain for chitin

15

Sabourad's Agar

Agar with 4% glucose and a pH of 5.6 favor mold and inhibit bacteria

16

Germ Tube Test

A 2 hour, sure fire way to ID pathogenic yeast. Combine with bovine serum, incubate and examine for germ tubes