Egg yolk agar
Detects Lipase, Lecithinase, and protease activities
Which Clostridium spp has a double zone of hemolysis?
C. Perfringens
Skim milk plates
Detects proteolysis of caesin protein by caseinase enzyme
Spirit Blue Plates
Contains lipids and an indicator of pH for detection of lipase
Nutrient Gelatin Tubes
Detects gelatin liquidification (gelatin protease activity)
1% Tryptone broth
Used for detection of indole production by tryptophanase
Litmus Milk Tubes
Provides lactose as a sugar source, Casein as a protein source, and looks for pH change
McFarland Turbidity Standard
0.5 standard that contains 99.5 ml of 1% sulfuric acid and 0.5 ml 1.175% barium chloride and is used to dilute an inoculum until its turbidity matches the sample
Mueller-Hinton Agar
Special agar for testing antimicrobial susceptability
Minimum Inhibitory Concentration
A tube dilution method of measuring antimicrobial susceptibility. Measured by the lowest concentration of a drug that inhibits growth
Minimal Bactericidal Concentration
The least concentration of an antimicrobial that is needed to kill 99.9% of organisms
Potato Dextrose Agar
A high sugar growth medium used to grow fungi with a slightly acidic pH to inhibit bacterial growth
10% KOH
Used in dermal isolates to dissolve keratinized cells leaving chitinous cell walls of fungi. Hyphae can be observed microscopically
Lactophenol cotton blue
Stain for chitin
Sabourad’s Agar
Agar with 4% glucose and a pH of 5.6 favor mold and inhibit bacteria
Germ Tube Test
A 2 hour, sure fire way to ID pathogenic yeast. Combine with bovine serum, incubate and examine for germ tubes
