Final Flashcards Preview

Medical Bacteriology Lab > Final > Flashcards

Flashcards in Final Deck (16)
Loading flashcards...
1
Q

Egg yolk agar

A

Detects Lipase, Lecithinase, and protease activities

2
Q

Which Clostridium spp has a double zone of hemolysis?

A

C. Perfringens

3
Q

Skim milk plates

A

Detects proteolysis of caesin protein by caseinase enzyme

4
Q

Spirit Blue Plates

A

Contains lipids and an indicator of pH for detection of lipase

5
Q

Nutrient Gelatin Tubes

A

Detects gelatin liquidification (gelatin protease activity)

6
Q

1% Tryptone broth

A

Used for detection of indole production by tryptophanase

7
Q

Litmus Milk Tubes

A

Provides lactose as a sugar source, Casein as a protein source, and looks for pH change

8
Q

McFarland Turbidity Standard

A

0.5 standard that contains 99.5 ml of 1% sulfuric acid and 0.5 ml 1.175% barium chloride and is used to dilute an inoculum until its turbidity matches the sample

9
Q

Mueller-Hinton Agar

A

Special agar for testing antimicrobial susceptability

10
Q

Minimum Inhibitory Concentration

A

A tube dilution method of measuring antimicrobial susceptibility. Measured by the lowest concentration of a drug that inhibits growth

11
Q

Minimal Bactericidal Concentration

A

The least concentration of an antimicrobial that is needed to kill 99.9% of organisms

12
Q

Potato Dextrose Agar

A

A high sugar growth medium used to grow fungi with a slightly acidic pH to inhibit bacterial growth

13
Q

10% KOH

A

Used in dermal isolates to dissolve keratinized cells leaving chitinous cell walls of fungi. Hyphae can be observed microscopically

14
Q

Lactophenol cotton blue

A

Stain for chitin

15
Q

Sabourad’s Agar

A

Agar with 4% glucose and a pH of 5.6 favor mold and inhibit bacteria

16
Q

Germ Tube Test

A

A 2 hour, sure fire way to ID pathogenic yeast. Combine with bovine serum, incubate and examine for germ tubes