Final (ch 3)

Question 1

Base pairs of DNA

Answer 1

A+T, G+C

Question 2

Base pairs of RNA

Answer 2

A+U, G+C

Question 3

Purines have this structure

Answer 3

double ring

Question 4

purines

Answer 4

A,G

Question 5

Pyrimidines

Answer 5

T,U,C

Question 6

Pyrimidines have this structure

Answer 6

single ring

Question 7

DNA (full name and why)

Answer 7

deoxyribose

-H at carbon 2 of pentose

Question 8

RNA (full name and why)

Answer 8

Ribose

-OH at carbon 2 of pentose

Question 9

The base attaches to this carbon

Answer 9

C1

Question 10

The phosphate attaches to the sugar at this carbons

Answer 10

C3

C5

Question 11

nucleoside =

Answer 11

base + sugar

Question 12

nucleotide _

Answer 12

base + sugar + phosphate

Question 13

nucleotides link thorugh these types types of bonds

Answer 13

5 - 3 phosphodiester bonds

Question 14

A+T hydrogen bonds

Answer 14

2

Question 15

G+C hydrogen bonds

Answer 15

#

Question 16

How long are the bonds

Answer 16

10.85A

Question 17

most abundant form of DNA in nature

Answer 17

B-DNA

Question 18

This DNA has 11 base pairs/turn

Answer 18

A-DNA

Question 19

Left handed helix in this DNA

Answer 19

Z-DNA

Question 20

these are both right handed helix DNA

Answer 20

B-DNA

A-DNA

Question 21

Eukaryotic DNA with this charge wraps around these with this charge resulting in this

Answer 21

negative
histones
postivie
chromatin

Question 22

each ‘bead’ on the string is this

Answer 22

nucleosome

Question 23

Melting point of DNA is this

Answer 23

temp 1/2 of DNA is denatured

Question 24

This is a reason DNA will have a higher melting point

Answer 24

[G-C] > [A-T]

Question 25

DNA to DNA

Answer 25

replication

Question 26

DNA to RNA

Answer 26

transcription

Question 27

RNA to protein

Answer 27

Translation

Question 28

RNA to DNA

Answer 28

Reverse transcription

Question 29

R to T to T =

Answer 29

replication to transcription to translation

Question 30

RNA transcription is made from this

Answer 30

non-coding DNA strand (3-5) howerver RNA is made 5-3

Question 31

encodes for pp sequence

Answer 31

mRNA

Question 32

carries AA to ribosome

Answer 32

tRNA

Question 33

aids in polypeptide synthesis

Answer 33

rRNA

Question 34

These lack 3' -OH group

Answer 34

ddNTP

Question 35

Method which uses ddNTP to sequence DNA

Answer 35

sanger method

Question 36

What is required to make PCR work

Answer 36

heat stable DNA polymerase

Question 37

PCR repeats these three chemical reactions

Answer 37

denaturation of dsNDA to ssDNA Annealing, primers can pase pair to ssDNA Extension, 72*C is optimal temp for heat stable DNA polymerase to work

Question 38

Role of restriction enzymes in nature

Answer 38

aid in defense for bacteria

Question 39

bacteria do this to their DNA, bacteriaphages do not

Answer 39

methylate their DNA

Question 40

EcoRI has this type of ends

Answer 40

sticky ends

Question 41

EcoRV has this type of sticky ends

Answer 41

blunt ends

Question 42

Gel for seperating DNA fragments by size is made of this

Answer 42

agarose

Question 43

these are naked DNA molecules

Answer 43

plasmids

Question 44

Plasmids are dependent on this

Answer 44

host cell's proteins for replication

Question 45

This is a method of introducing single AA protein as a result of a mucation at a specific site in the DNA

Answer 45

side-detected mutagenesis

Question 46

incorporation of what would result in chain termination during sequencing of DNA

Answer 46

ddNTP

Question 47

The 5' end of a polynucleotide contains this while the 3' end contains this

Answer 47

posphate group | hydroxyl group

Question 48

the backbone of a nucleic acid consists of

Answer 48

phosphodiester bond between the 3' and 5' hydroxyl gorups of neighboring sugars

Question 49

Name a molecule that contains a phosphoanhydride bond

Answer 49

ATP

Question 50

What is the correct order of steps in a PCR reaction beginning with double stranded DNA

Answer 50

Strand separation primer annealing primer extenstion

Question 51

what term describes a small circular molecule of DNA that can be sued to transfer genetic material from one organism to the other

Answer 51

plasmid