Final Practical

Question 1

killing/control of microorganisms divided into two groups: _____ and ____ methods

Answer 1

chemical and physical methods

Question 2

physical: ____ and _____

Answer 2

heat and UV light

Question 3

chemical: ____ and ____

Answer 3

non biological and biological

Question 4

_______ are chlorine, bromine, acids, heavy metals, formaldehyde

Answer 4

non biological

Question 5

an example of biological

Answer 5

antimicrobials

Question 6

way we determine if something has killed a microorganism is to ________

Answer 6

see if it grows after treatment

Question 7

bacillus was the most resistant to boiling (because it formed _____)

Answer 7

spores

Question 8

___ and ____ were killed in the first min of irradiation

Answer 8

EC and PA

Question 9

number of colonies of ____ was diminished by UV, but there were still a lot of colonies after two minutes of treatment (due to spores)

Answer 9

BC

Question 10

in the kirby bauer experiment a ____ plate was used

Answer 10

Mueller Hinton plate

Question 11

following incubation we measured the _______ to figure out if a microorganism was _____ to a drug, ______ to a drug, or _____

Answer 11

following incubation we measured the ZONE DIAMETERS to figure out if a microorganism was RESISTANT to a drug, SUSCEPTIBLE to a drug, or INETRMEDIATE

Question 12

in the hand washing experiment we used a ___ plate

Answer 12

nutrient agar

Question 13

Added 0.1 ml of the solution to 100 ml of sterile saline, this is a ____ dilution

Answer 13

1/1000

Question 14

NA is a general media and _______

Answer 14

all of the organisms should grow on it

Question 15

VRBA contains chemicals that inhibit the growth of the ______

Answer 15

Gram-positive bacteria

Question 16

VRBA allows mostly _____ (such as E. coli and other coliforms) to survive

Answer 16

gram negatives

Question 17

A positive VRBA plate will have organisms growing and they will be ___

Answer 17

red

Question 18

for the 1 ml plates, the number of colonies found are multiplied by ____ to give you the number found per mL in the original sample

Answer 18

1,000

Question 19

____ agar was added to a meat sample

Answer 19

Hektoen enteric agar (Enteric)

Question 20

HE is a selective and differential agar used to identify _____ and _____

Answer 20

Salmonella and Shigella

Question 21

Salmonella will produce ____ colonies

Answer 21

black

Question 22

Shigella will produce _____ colonies

Answer 22

translucent green

Question 23

two types of staph

Answer 23

S. epidermidis, S. aureus

Question 24

two types of enterococcus

Answer 24

E. faecalis, E. gallium

Question 25

4 types of strep

Answer 25

S. agalactiae (Group B), S. mutans, S. equis, S. pyogenes (Group A)

Question 26

to tell if you have staph or strep you first do the ____ test

Answer 26

catalase

Question 27

in the catalase test we added organisms to _____

Answer 27

hydrogen peroxide

Question 28

If organisms had catalase the peroxide was broken down into _____ and ____ (we could see this because the oxygen bubbled)

Answer 28

water and oxygen

Question 29

Staphylococci are CATALASE ____

Answer 29

POSITIVE

Question 30

Streptococci are CATALASE ____

Answer 30

NEGATIVE

Question 31

Streptococci and Staphylococci are _____

Answer 31

gram positive cocci

Question 32

enterococcus were formerly

Answer 32

group d strep

Question 33

Normally one separates the Staph into species based on the ____ test

Answer 33

COAGGULASE TEST

Question 34

In a latex test, an _____ is placed on the surface of a latex bead

Answer 34

antibody

Question 35

If the bacteria you are looking for is present in the sample, it binds to the latex and forms ____

Answer 35

clumps

Question 36

____ test helps us separate S. aureus from other Staph

Answer 36

mannitol salt

Question 37

Mannitol Salt is a ____ plate which allows ___ to grow

Answer 37

differential plate which allows staph to grow

Question 38

S. aureus will turn the mannitol salt plate ____

Answer 38

yellow

Question 39

a positive (clumping) latex test means you have ____

Answer 39

staph aureus

Question 40

Historically Strep were identified on the basis of what they did to the ___ in _____ plates

Answer 40

blood cells in Blood Agar plates

Question 41

Alpha Hemolysis will be ______

Answer 41

transparent and green

Question 42

Beta Hemolysis will have a ____

Answer 42

clear area

Question 43

there could be ____, ____ or __ hemolysis

Answer 43

alpha, beta, or none

Question 44

One way of identifying Enterococci are by doing the _____ test

Answer 44

Bile Esculin Test

Question 45

Bile Esculin is a _____ (it only allows certain organisms to grow)

Answer 45

differential media

Question 46

Enterococci (and Group D Strep) will turn the bile esculin agar ___

Answer 46

black

Question 47

bile esculin negatives are that sort of ____

Answer 47

greenish brown

Question 48

____ are responsible for causing some of the most famous diseases in history

Answer 48

gram negative rods

Question 49

gram negative rods are responsible for ___, ____, ____

Answer 49

Typhoid Fever, Cholera, Bubonic Plague

Question 50

MacKonkey plate: differential plate for _____

Answer 50

lactose fermenting Gram negative rods

Question 51

on the mackonkey plate positive colonies are

Answer 51

purple

Question 52

Eosin Methylene Blue plates differential plate for ________

Answer 52

lactose fermenting Gram negative rods

Question 53

on eosin methylene blue plates e coli produces a

Answer 53

distinctive fluorescent green color

Question 54

Triple Sugar Iron tubes (TSI) does three tests: 1. 2. 3.

Answer 54

1. Fermentation test 2. Production of hydrogen sulfide 3. Production of gas

Question 55

Fermentation test: Positive forms ___ and is ___

Answer 55

acid and is yellow (That’s what the “A” stands for)

Question 56

Fermentation test: negative is ___

Answer 56

red (Abbreviated as K)

Question 57

Production of hydrogen sulfide: Positive reacts with iron and turns ___

Answer 57

black

Question 58

Production of gas causes ____

Answer 58

air bubbles

Question 59

20E test is for

Answer 59

gram negative rods

Question 60

anaerobes _______

Answer 60

cant grow where air is

Question 61

Neisseria are _____ organisms and as a result only grow on plates with lots of extra nutrition

Answer 61

fastidious

Question 62

N. sicca grew on _____ agar

Answer 62

chocolate

Question 63

We inoculated soil anaerobes into ____

Answer 63

Thioglycolate broth

Question 64

This broth contains chemicals that destroy the ____

Answer 64

oxygen

Question 65

______ grow on top

Answer 65

Aerobes

Question 66

anaerobes on the ____

Answer 66

bottom

Question 67

______ in the middle

Answer 67

facultative anaerobes

Question 68

Some of the tubes had a clear zone on the top of the tube. This meant that the sample we inoculated had NO ______, so nothing grew on the top

Answer 68

aerobes

Question 69

urine test used which 3 plates

Answer 69

blood agar, mackonkey, pea

Question 70

Blood agar plate shows ____ because _____

Answer 70

total number of organisms because everything grows on it

Question 71

MacKonkey plate: This is a differential media plate so it shows only the number of ______

Answer 71

gram negatives

Question 72

PEA plate:This is a differential media plate so it shows only the number of ____

Answer 72

gram positive

Question 73

plates used to collect mold

Answer 73

sabouraud dextrose plate`

Question 74

tape preparation of plates used

Answer 74

lactophenol

Question 75

Fungi are ____

Answer 75

eukaryotes

Question 76

____: single cell, has nucleus, big

Answer 76

yeast

Question 77

___: single cell that generates tube called hyphae

Answer 77

mold

Question 78

Multiple hyphae are ____

Answer 78

mycelium