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1

aseptic technique is a technique used to prevent...

microbial contamination

2

what does aseptic technique entail?

being ready for experiment by having a sterile environment and wearing personal protective gear. being neat and organized and washing your hands

3

how many feet must you work within the bunsen burner?

1.5 to 2 feet. also don't forget to flame everything prior to inoculation.

4

c1v1 = c2v2

c1=stock concentration v1=added volume c2=desired concentration v2=total volume

5

1% =

1.0g/100ml

6

compound microscope use what as the source of illumination? who invented microscope?

light; antony van leeuwenhoek with a mag of 270x

7

ocular lens

enlarges primary image of the specimen. 10x magnification.

8

objective lens

produces the primary image. 10x, 40x, 100x.

9

use safety goggles when..

using flame, hot plate, or any kind of chemical

10

where do you dispose the serological pipettes?

tips down in the pipette jar

11

magnification? resolution? limit?

how much an image in enlarged, detail of the image, 2um

12

what arrangements do cocci come in? rods?

single, pair, tetrad, chain and clusters. single, chain.

13

what is the proper storage of a microscope?

stage is flushed and all the way down, objective lens at 4x, the cord wrapped, and light off.

14

"The role of the infinitely small in nature is infinitely large"

Luis Pasteur

15

Microbes include:

Bacteria/archea, protozoa, viruses, fungus, unicellular algae

16

what does one need to provide microbes in order for them to grow?

adequate nutrition, and suitable growth environment conditions

17

many species of bacteria may be present in the environment in physiological state. we call this

viable but nonculturable

18

Good places we find microbes:

agriculture, biotechnology, food, environment

19

bad places we find microbes:

food spoilage, food poisoning, diseases: epidemics/pandemics, std

20

what grows on agar to show us number and variety of microbes present?

colonies

21

what is using a single dye to see general morphology, shape, and arrangement of cells?

simple staining (use crystal violet or carbol-fuchsin

22

differential staining? structural staining?

gram staining, spore/flagella staining

23

a suspension of cells in water, which is dried on a slide

smear

24

what microscope magnification can you see bacteria? protozoa?

1000x, 100-400x

25

p

staphylococcus, e coli

26

p

arechea: halococcus 1000x

27

p

protozoa: chlorella 1000x

28

p

Euglena 400x

29

p

fungi: penicillium 400x

30

what are the 6 basic nutritional requirements?

energy, carbon, nitrogen, vitamins, minerals, water