aseptic technique is a technique used to prevent...
what does aseptic technique entail?
being ready for experiment by having a sterile environment and wearing personal protective gear. being neat and organized and washing your hands
how many feet must you work within the bunsen burner?
1.5 to 2 feet. also don't forget to flame everything prior to inoculation.
c1v1 = c2v2
c1=stock concentration v1=added volume c2=desired concentration v2=total volume
compound microscope use what as the source of illumination? who invented microscope?
light; antony van leeuwenhoek with a mag of 270x
enlarges primary image of the specimen. 10x magnification.
produces the primary image. 10x, 40x, 100x.
use safety goggles when..
using flame, hot plate, or any kind of chemical
where do you dispose the serological pipettes?
tips down in the pipette jar
magnification? resolution? limit?
how much an image in enlarged, detail of the image, 2um
what arrangements do cocci come in? rods?
single, pair, tetrad, chain and clusters. single, chain.
what is the proper storage of a microscope?
stage is flushed and all the way down, objective lens at 4x, the cord wrapped, and light off.
"The role of the infinitely small in nature is infinitely large"
Bacteria/archea, protozoa, viruses, fungus, unicellular algae
what does one need to provide microbes in order for them to grow?
adequate nutrition, and suitable growth environment conditions
many species of bacteria may be present in the environment in physiological state. we call this
viable but nonculturable
Good places we find microbes:
agriculture, biotechnology, food, environment
bad places we find microbes:
food spoilage, food poisoning, diseases: epidemics/pandemics, std
what grows on agar to show us number and variety of microbes present?
what is using a single dye to see general morphology, shape, and arrangement of cells?
simple staining (use crystal violet or carbol-fuchsin
differential staining? structural staining?
gram staining, spore/flagella staining
a suspension of cells in water, which is dried on a slide
what microscope magnification can you see bacteria? protozoa?
staphylococcus, e coli
arechea: halococcus 1000x
protozoa: chlorella 1000x
fungi: penicillium 400x
what are the 6 basic nutritional requirements?
energy, carbon, nitrogen, vitamins, minerals, water