FISH ADVANTAGES AND DISADVANTAGES

Question 1

What is a key advantage of FISH regarding metaphases?

Answer 1

No need to yield metaphases

This allows for quicker analysis as metaphase chromosome spreads are not required.

Question 2

How quickly can FISH provide results?

Answer 2

Rapid result over night

This is beneficial for timely diagnosis and treatment planning.

Question 3

What is a significant capability of FISH in terms of translocations?

Answer 3

Several translocations in a single assay

This allows for comprehensive analysis of multiple genetic alterations at once.

Question 4

What types of translocations can FISH detect?

Answer 4

Detect all cryptic and variants translocations

This includes translocations that may not be easily identified by other methods.

Question 5

What is a limitation of FISH regarding the regions assessed?

Answer 5

Need to know what you are looking for

FISH will only assess that particular region at a time, potentially missing additional aberrations.

Question 6

What is a cost-related limitation of FISH?

Answer 6

Costly

The expense of FISH can be a barrier to its widespread use.

Question 7

What is the sensitivity range of cells analyzed in FISH?

Answer 7

200-500

This is better sensitivity than conventional cytogenetics which analyzes 1 in 20 cells.

Question 8

True or False: FISH can detect additional aberrations beyond the target region.

Answer 8

False

FISH only assesses the specific region known in advance.

Question 9

Fill in the blank: FISH has better sensitivity than conventional cytogenetics with an analysis of _______ cells.

Answer 9

200-500

This indicates a higher efficiency in detecting genetic abnormalities.