Fish Quality Flashcards

(63 cards)

1
Q

sensory, microbial, and chemical quality indices

A

odour
appearance
texture
flavour

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2
Q

how many phases of deterioration are there for fish

A

4

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3
Q

describe phase 1

A

fresh
characteristic odour
delicate taste

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4
Q

how long does phase 1 last

A

almost 2 days

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5
Q

describe phase 2

A

loss of odour and taste
autolysis begins

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6
Q

what is autolysis

A

biochemical changes and degradation of proteins, nucleotides and molecules

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7
Q

days of phase 2

A

2-6

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8
Q

describe phase 3

A

‘fishy’ odour associated with
microbial spoilage
enzymatic reactions

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9
Q

days that phase 3 occur

A

6-12

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10
Q

describe phase 4

A

putrid odour, mushy/soft texture
advanced proteolysis

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11
Q

days that phase 4 occurs

A

> 12 days

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12
Q

what are cephalopods

A

squids etc

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13
Q

how does glycolysis occur in fish

A

-6C and 3C molecules are transformed to pyruvate
-pyruvate is used in TCA cycle and converted to lactate (except cephalopods)

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14
Q

what is pyruvate converted to in cephalopods

A

octopine

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15
Q

consequences of octopine conversion over lactate

A

results in a higher ultimate pH

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16
Q

why is the pH drop in fish less than terrestrial animals

A

less glycogen stored

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17
Q

typical pH fall in fish

A

6.8-7.2 intially
drops to 6.1-6.9

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18
Q

% of glycogen stores in land animals vs fish

A

2-1% in land
0.5% in fish

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19
Q

when does rigor develop in fish

A

2 hrs after death

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20
Q

when does rigor resolve in fish

A

1 day

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21
Q

what is the rate of onset of rigor determined by

A

temperature (cold or tropical fish)
condition
handling
size

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22
Q

is rigor desired in
-fish
-red meat

A

yes in fish
no in reed meat

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23
Q

why is rigor wanted to be preserved in fish

A

the firmness of fish makes it more difficult for bacteria to move to the surface

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24
Q

what ‘condition’ could result in low glycogen stores in fish

A

spawning

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25
types of physical and biochemical changes associated with the rate of rigor that affects the quality
gapping proteolysis
26
what is gapping caused by
rapid onset of rigor
27
when does gapping occur
when cold water fish are stored at too high of a temp it can cause rapid onset of rigor
28
what happens during gapping
strong contraction cause weakening of CT (mycommata) which ruptures the fillet resulting in the segementation of myotomes
29
what does proteolysis of hoki cause
softening
30
which protease is calcium dependant and involved in Hoki softening
calpains
31
how can the deterioration of Hoki be slowed
rapid chilling from 12-1 degrees
32
what is nucleotide degradation
degradation of ATP to biochemical compounds that affect sensory attributes
33
reaction scheme of ATP degradation
ATP-> ADP->AMP->IMP->inosine->hypoxanthine
34
how does AMP-> IMP
deamination
35
which product is desirable in nucleotide degradation
inosine monophosphate (IMP)
36
what flavor does hypoxanthine produce
bitter
37
difference betwen inosine andIMP
not much but IMP is better
38
how is inosine broken down
phosphotase enzyme
39
what is a K factor
freshness index/spoilage indicator
40
what are the components of K factor equation
ino and Hx/ total ATP related products
41
what does a high K factor indicate
less fresh
42
5 autolytic changes in fish
glycolysis nucleotide breakdown proteolysis lipolysis//autooxidation TMAO breakdown
43
enzymes and substrate of glycolysis
glycolytic enzymes glycogen
44
enzymes and substrate of nucleotide breakdown
endogenous autolytic enzymes ATP, ADP, AMP, IMP,
45
enzymes and substrate of proteolysis
calpains and cathepsins proteins, peptides myofribrills, CT
46
enzymes and substrate of lipolysis
lipases and phospholipases lipid and phospholipids
47
enzymes and substrate of autoxidation
lipoxygenases PUFAs
48
enzymes and substrate of TMAO breakdown
TMAO demethylase TMOA
49
how to prevent/ inhibit glycolysis causes defective changes
allow the fish to pass through rigor at temps close to 0
50
how to prevent/ inhibit nucleotide breakdown
low temp careful handling
51
how to prevent/ inhibit proteolysis
careful handling low temp
52
what is TMAO
a substance present naturally stablises intracellular proteins and denaturation of urea
53
process of TMAO breakdown
reduced by bacteria to TMA and enzymatically converted to DMA and formaldehyde
54
changes encountered by TMAO breakdown
toughening
55
what does TMA cause
fishy off flavour
56
what does formaldehyde do
induces protein cross-linking
57
shelf life of frozen fish
3 months
58
what does longer freezer storage of protein cause
less extractable protein
59
impact of freezing on fish
protein denaturation and texture deterioration
60
what do proteins interact with during degradation
water, lipids and endogenous enzymes
61
examples of texture deterioration from freezing
toughening drying/ loss of succulence
62
examples of flavour deterioration from freezing
development of off-flavours from -lipid hydrolysis and oxidation -formation TMA
63
examples of loss of protein functional properties from freezing
loss in WHC, solubility, gelling capability, lipid emulsifying capability