Flow Colors Flashcards
(27 cards)
1
Q
Purple wavelength
A
400 nm
2
Q
Blue wavelength
A
450 nm
3
Q
Green wavelength
A
500-550 nm
4
Q
Yellow wavelength
A
600 nm
5
Q
Red wavelength
A
700 nm
6
Q
Is a lower wavelength higher or lower enegry?
A
Higher energy
7
Q
Is a higher wavelength higher or lower energy?
A
Lower energy
8
Q
FITC excitation
A
490
9
Q
FITC emission
A
525
10
Q
FITC color
A
Green
11
Q
PE excitation
A
496 or 546
12
Q
PE emission
A
578P
13
Q
PE color
A
Yellow
14
Q
PerCP excitation
A
490
15
Q
PerCP emission
A
525
16
Q
PerCP color
A
Green
17
Q
APC excitation
A
650
18
Q
APC emission
A
661
19
Q
APC color
A
Yellow/Red
20
Q
DAPI excitation
A
359
21
Q
DAPI emission
A
461
22
Q
DAPI color
A
Blue
23
Q
AF700 excitation
A
696
24
Q
AF700 emission
A
719
25
AF700 color
Red
26
What factor of a fluorophore informs the laser?
excitation
27
What factor of a fluorophore informs the filter?
emission
