Fresh Tissues (Histopath) Flashcards
(38 cards)
protoplasmic activities
motion, mitosis,
phagocytosis, and pinocytosis
Selected tissue specimen is immersed in a watch
glass containing isotonic salt solution
Teasing or Dissociation
stain used in teasing
supravital dye
more advantage
than brightfield because it greatly increases the
structural detail of the cells examined
Phase Contrast Microscope
● Small pieces of the tissue is placed in between 2 glass slides and compressed
● Vital dye is place in junction of the slides and
absorbed through capillary action by the tissue
Squash or Crushing
size of tissue in squash
not more than 1mm in
dm
In this method, sections or sediments are spread
lightly over a slide by means of wire loop or
applicator stick or by making an apposition smear
with another slide.
Smearing
Smearing is especially
useful in what exam
cytological examination particularly for
diagnosis of cancer
less suitable for examination
Too thin or too thick smear must be
avoided
smearing is also useful for thick secretion fluids such as
serous fluid, enzymatic lavage from GI tract,
concentrated sputum, blood and bronchial lavage
A selected portion of the material is transferred to
a clean slide and gently spread into a moderately
thick film by using circular motion
Spreading
Section or sediments are spread light over a slide;
ZIGZAG OR DIRECT LINE
Streaking
- Place a drop of secretion or sediment upon one
slide and facing into another clean side - Sample is placed in the middle of the glass slides
- The material disperse evenly over the surface of
two slides. Slight movement of the two slides in
opposite direction may be necessary to initiate
the flow of materials
Pull Apart
● The surface of a freshly cut piece of tissue is
brought into contact and pressed lightly unto the
surface of a clean glass slide, allowing the cells
to be transferred directly to the slide for
examination.
● ADVANTAGE: Cells may be examined without
destroying their intracellular relationship
Touch Preparation
RAPID DIAGNOSIS of the tissue is in question is
required, and is especially recommended when
lipid and nervous tissue elements are to be
demonstrated
Frozen Section
Slices size a fresh tissue or frozen on a microtome with CO2, or on a cryostat
10-15u in thickness
Cryostat: cold chamber kept at what atmospheric temperature
-10 C to -20 C
It is usually used in specimens that has a lot of
lipids and nerve tissues
Frozen Section
Freezing agents
CALI (CO2, Aerosol spray, Liquid
nitrogen, Isopentane cooled by liquid
nitrogen)
FIRST and MOST CRITICAL step
FIXATION
primary objective of fixation
preserve the morphologic and
chemical integrity of the cell
■ Inactivates lysosomal enzyme
activity
seocndary objective of fixation
harden and protect tissue
from trauma
○ Kills microorganisms and prevents molds
Main Factors Involved
● Volume
● pH
● Temperature
● Thickness of the section
● Osmolality
● Concentration
● Duration of Fixation
● Time interval
Fixative Volume
Sample Volume
