Functions of a Laboratory Assistant/ Aide: Removing Grease 1. Boil the glassware in a weak solution of ______, __________, or any fat solvent 2. For silicone grease, soak the stopcock plug or barrel in warm ________ for two (2) hours. 3. Drain, rinse with ________, or use ____________ for 30 minutes. 4. ________ all of the cleaning agents.

1. sodium carbonate, acetone 2. decahydronaphthalene 3. acetone, fuming sulfuric acid 4. Rinse off

Washing laboratory glassware: Pipets 1. Place pipets with ____ in a cylinder or tall jar of water. 2. Immerse most if not the ____ into the water. 3. Drain and place in a cylinder or jar of dissolved detergent. (Soak in a jar of ____ if dirty) 4. Drain. Run ____ over and through the pipets. 5. Soak in distilled water for at least ____ (time), then remove 6. Rinse and dry the ____ portion using a cloth. 7. ____ the water out of the pipets and let them dry.

1. tips down 2. whole pipet 3. chromic acid cleaning solution 4. tap water 5. one hour 6. outside 7. Shake

Washing laboratory glassware: Culture tubes 1. Autoclave for ____ at ____ 2. If there are media that solidify upon cooling, pour them out while ____. 3. Empty the ____. 4. Brush with ____ and water. Rinse with tap water thoroughly. 5. Rinse using ____. 6. Place the tubes in a ____ and let dry.

1. 30 minutes at 121 °C (15 p.s.i. pressure). 2. the tubes are still hot 3. tubes 4. detergent 5. distilled water 6. basket

Washing laboratory glassware: Petri dishes and culture bottles 1. ____ and clean following the procedure for cleaning culture tubes. 2. Wrap in heavy paper or place in a ____. 3. Sterilize the dishes and culture bottles using the ____ or ____

1. Sterilize 2. petri dish can 3. autoclave or a dry-air sterilizer

Sterilizing and storing glassware: Serology tubes and culture media place in a ____ or ____ add ____ or ____ then boil for ____ minutes rinse with ____ scrub with ____ ____

large bucket or boiler 1%–2% soap or detergent 30 tap water detergent rinse again

Sterilizing and storing clean glassware: Drying dried by hanging on ____ air-dried by placing in baskets with ____ drying oven with drying temp. below ____

wooden pegs mouths downward 140 °C

Guidelines in the maintenance of the laboratory work areas 1. Floors should be ____. There must be no objects or spilled materials on the floor 2. Materials should be returned to their designated places after ____. 3. The laboratory exit floor plan should have ____ 4. Safety showers, eyewashes, fire extinguishers, and electrical control boxes should be accessible and ____. 5. Sink traps and floor drains should be maintained and filled with water to avoid ____ 6. ____ should be placed away from any edges 7. The cleaning of the lab area should be ____ (completion of tests or at the end of the working day) 8. Bench tops and liners are kept free from ____. 9. ____ should be cleaned immediately to prevent slips, trips, and falls 10. Doors and drawers are kept closed and passageways should be free from ____

1. clutter-free 2. use 3. two clear passageways 4. strategically placed 5. sewer gases from escaping 6. Bench apparatus 7. scheduled 8. visible contamination 9. Spills 10. obstruction

Function of lab aide, Lab waste Management plan Flashcards by Do Re Mi

The Functions of a Laboratory Assistant/ Aide

2 job assignments of laboratory assistants

receptionist in the reception area
laboratory aide in testing areas

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6 Functions of a Laboratory Assistant/ Aide

washes laboratory glassware
cleans laboratory workbenches
helps in mixing chemical solutions
segregates laboratory wastes
restocks the laboratory supplies
operates autoclaves
maintains the cleanliness of laboratory
equipment and the laboratory in general

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Functions of a Laboratory Assistant/ Aide

Laboratory glasswares should be …? (3)

clean (physically and chemically)
sterile
grease-free

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Functions of a Laboratory Assistant/ Aide

6 steps on washing laboratory glassware

Rinse with water
soak in detergent solution, then brush
rinse with tap water
rinse with distilled water
dry (air dry or drying oven)
store in a cabinet

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Functions of a Laboratory Assistant/ Aide: Removing Grease

Boil the glassware in a weak solution of ______, __________, or any fat solvent
For silicone grease, soak the stopcock plug or barrel in warm ________ for two (2) hours.
Drain, rinse with ________, or use ____________ for 30 minutes.
________ all of the cleaning agents.

sodium carbonate, acetone
decahydronaphthalene
acetone, fuming sulfuric acid
Rinse off

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Identify the laboratory instrument

rinse using running tap water. Rinse with distilled water or use a large bath of distilled water.

Glassware

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Identify the laboratory instrument

allow the tap water to run into and over them
partly fill each piece with water, shake, and empty for about six (6) times.

Test tubes, graduated cylinders, and flasks

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Identify the laboratory instrument

attach a piece of tubing to the faucet, then connect the delivery end to allow water to run through them.

Pipets

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Washing laboratory glassware: Pipets

Place pipets with ____ in a cylinder or tall jar of water.
Immerse most if not the ____ into the water.
Drain and place in a cylinder or jar of dissolved detergent. (Soak in a jar of ____ if dirty)
Drain. Run ____ over and through the pipets.
Soak in distilled water for at least ____ (time), then remove
Rinse and dry the ____ portion using a cloth.
____ the water out of the pipets and let them dry.

tips down
whole pipet
chromic acid cleaning solution
tap water
one hour
outside
Shake

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Washing laboratory glassware: Culture tubes

How long and at what temperature should we autoclave culture tubes? What pressure?

30 minutes at 121°C (15 psi pressure)

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Washing laboratory glassware: Culture tubes

Autoclave for ____ at ____
If there are media that solidify upon cooling, pour them out while ____.
Empty the ____.
Brush with ____ and water. Rinse with tap water thoroughly.
Rinse using ____.
Place the tubes in a ____ and let dry.

30 minutes at 121 °C (15 p.s.i. pressure).
the tubes are still hot
tubes
detergent
distilled water
basket

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Washing laboratory glassware: Petri dishes and culture bottles

____ and clean following the procedure for cleaning culture tubes.
Wrap in heavy paper or place in a ____.
Sterilize the dishes and culture bottles using the ____ or ____

Sterilize
petri dish can
autoclave or a dry-air sterilizer

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Sterilizing and storing glassware: Serology tubes and culture media

What should you do before cleaning serology tubes and culture media?

Sterilize before cleaning

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Sterilizing and storing glassware: Serology tubes and culture media

Examples of equipment that can be used to sterilize the glassware

autoclave
large steam oven
or similar equipment

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Sterilizing and storing glassware: Serology tubes and culture media

place in a ____ or ____
add ____ or ____ then boil for ____ minutes
rinse with ____
scrub with ____
____

large bucket or boiler
1%–2% soap or detergent
30
tap water
detergent
rinse again

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Sterilizing and storing clean glassware: Drying

dried by hanging on ____
air-dried by placing in baskets with ____
drying oven with drying temp. below ____

wooden pegs
mouths downward
140 °C

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Sterilizing and storing clean glassware: Drying

Two things to do to ensure that the vessel mouth is clean

drying basket should be lined with a clean cloth
pipets and cylinders are dried by standing them on a folded towel

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Storing clean glassware

protect from dust by plugging with cotton, ____, or placing a heavy piece of paper over the mouth and sealing them with ____
store in specially designed ____
alkaline liquids should not be stored in flasks because the stoppers or ____ may stick

corking, tape
racks
stopcocks

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Storing clean glassware:

What should you do to contaminated glassware to sterilize them?

Autoclave

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Storing clean glassware:

After autoclaving a contaminated glassware, what should you do?

Airdry or use a drying oven

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Cleaning and Maintenance Procedure of the Laboratory Work Areas

2 reasons why we should maintain a clean environment

to reduce the risk of infection
to reduce cross-contamination during the specimen handling

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Cleaning and Maintenance Procedure of the Laboratory Work Areas

Clean the surfaces of laboratory equipment regularly. Comply with the ____ of the institution

operating procedures

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Cleaning and Maintenance Procedure of the Laboratory Work Areas

What should you document using a laboratory logbook?

Cleaning and maintenance activities

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Guidelines in the maintenance of the laboratory work areas

Floors should be ____. There must be no objects or spilled materials on the floor
Materials should be returned to their designated places after ____.
The laboratory exit floor plan should have ____
Safety showers, eyewashes, fire extinguishers, and electrical control boxes should be accessible and ____.
Sink traps and floor drains should be maintained and filled with water to avoid ____
____ should be placed away from any edges
The cleaning of the lab area should be ____ (completion of tests or at the end of the working day)
Bench tops and liners are kept free from ____.
____ should be cleaned immediately to prevent slips, trips, and falls
Doors and drawers are kept closed and passageways should be free from ____

clutter-free
use
two clear passageways
strategically placed
sewer gases from escaping
Bench apparatus
scheduled
visible contamination
Spills
obstruction

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# Guidelines in the maintenance of the laboratory work areas Keep ____ clear for emergencies

Hallways

# Cleaning and Maintenance Procedure * fresh ____ prepared daily * NOT stored in ____ * ____ properly (name and date of preparation)

* cleaning reagents * diluted form * labeled

# Cleaning and Maintenance Procedure What reagent is used for daily cleaning?

1% sodium hypochlorite solution

# Cleaning and Maintenance Procedure How to prepare 1% sodium hypochlorite solution for daily cleaning?

Add 10 mL of sodium hypochlorite solution to 1000 mL of water

# Cleaning and Maintenance Procedure How to prepare 70% ethanol

Take 700 mL of absolute ethanol and add 300 mL of distilled water

# Cleaning and Maintenance Procedure How to prepare Lysol (5% phenolic solution)

1 liter of 5% lysol is produced by adding 450 mL 12% lysol to 550 mL of distilled water

# Cleaning and Maintenances: **Daily cleaning procedure** * Fresh cleaning solutions should be ____ * ____ should be worn when cleaning the area * pieces of ____ are cleaned according to the SOP * materials that prevent ____ are removed * ____ is poured and spread on the work surface with paper towel

* prepared * PPE * equipment * thorough cleaning * 5% Lysol solution

# Cleaning and Maintenance Procedure: **Weekly cleaning of the work area** What cleaning material/reagent is used?

soap solution followed by 5% lysol solution

# Cleaning and Maintenance Procedure: **Weekly cleaning of the work area** * Using soap solution followed by ____ * Cleaning the ultrasonic water bath using ____ and refilling with fresh distilled water. * Cleaning the racks and tubes on a weekly basis by immersing them in ____ and allowing them to ____. * The racks for the molecular testing are cleaned after use by immersing them in ____

* 5% Lysol solution * hypochlorite (bleach) solution * 5% Lysol, air-dry * 1% sodium hypochlorite (bleach) solution.

# Laboratory waste management plan Waste Management Hierarchy preferable to least preferable (4)

1. Green chemistry 2. Reuse, redistribute, and reduce 3. recycle 4. environmental fate of waste

# Waste Management Hierarchy pollution prevention, and source reduction

green chemistry

# Waste Management Hierarchy * reuse unwanted materials * redistribute surplus chemicals * reduce hazards

reuse, redistribute, and reduce

# Waste Management Hierarchy Recycling chemical and recover energy from waste

recycle

# Waste Management Hierarchy incineration, land disposal, and other treatment methods

environmental fate of the waste

# Waste hierarchy 5 Waste hierarchy (most unfavorable to most favorable)

1. reduce 2. reuse 3. recycle 4. recovery 5. disposal

# 5 Waste hierarchy minimising the amount of waste produce

reduce

# 5 Waste hierarchy using waste materials to make new products

recycle

# 5 Waste hierarchy Using products repeatedly

reuse

# 5 Waste hierarchy obtaining energy from waste

recovery

# 5 Waste hierarchy landfill and incineration without energy recovery

disposal

What is the primary consideration of waste disposal

safety

# Properly handling of laboratory-accumulated waste You should have a clear plan for what?

disposal of hazardous and non-hazardous materials

# Properly handling of laboratory-accumulated waste Refers to a temporary storage for waste in the laboratory (controlled by a competent laboratory personnel)

satellite accumulation area

# Hazardous waste satellite accumulation area Containers must be ____ except when physically adding waste

closed at all times

# Hazardous waste satellite accumulation area containers must be labeled with all ________

chemical constituents

# Hazardous waste satellite accumulation area What containment should you use?

secondary containment (tray or tub)

# Hazardous waste satellite accumulation area What should you segregate?

incompatible wastes

# Proper handling of laboratory-accumulated waste These are used for spills and leakages from any primary container (ex.: trays)

secondary containment devices

# Proper handling of laboratory-accumulated waste Waste materials in the satellite accumulation should not be kept for more than how long?

not be kept for more than a year

# Proper handling of laboratory-accumulated waste Waste exceeding the ____ limit should be managed accordingly within 3 days

55-gal (or 1-qt)

# Proper handling of laboratory-accumulated waste Waste exceeding the 55-gal (or 1-qt) limit should be managed accordinly within how many days?

3 days

# Proper handling of laboratory-accumulated waste Collect ____ or ____ in containers pending transfer or disposal

* hazardous or flammable waste solvent

# Proper handling of laboratory-accumulated waste What should you do to avoid the occurence of heat generation, gas evolution, or other reactions?

Do not mix incompatible waste

# Proper handling of laboratory-accumulated waste ____ wastes according to how they will be managed

segregate

# Proper handling of laboratory-accumulated waste What should you observe?

Compatibility of containers and the waste contents

# Chemical waste containers 3 possible containers available/can be used?

* plastic * glass * metal

# Chemical waste containers Leave adequate ____ in liquid containers

headspace

# Chemical waste containers How many headspace should you have for a 4L container?

1 inch

# Proper Handling of Laboratory-accumulated Waste * Provide a container for collection of ____ * ____ such as amines should not be stored in metal containers * Do not use ____ for halogenated waste solvents to avoid corrosion and leaks

* liquid waste * corrosive materials * galvanized steel safety can

# Proper Handling of Laboratory-accumulated Waste * Labale the waste containers with their ____ * Keep the waste containers ____ * Separately collect ____ from organic solvent wastes. Collect in a container that is resistant to ____ * Do not use ____ if there is a danger of freezing

* contents * closed * aqueous wastes, corrosion * glass

# Proper Handling of Laboratory-accumulated Waste * Place ____ in a labeled container prior to disposal * Dispose ____ using the laboratory trash or segregate them for recycling

* Solid chemical wastes * non-hazardous solid wastes

# ProperHandlingof Laboratory-accumulated Waste:Disposalofempty containers * Remove the ____ and empty the contents * ____ the container properly if necessary * follow the ____ for disposing empty containers

* labels * rinse * laboratory guidelines

# Categories of health care wastes * Not in contact with infectious agents, hazardous chemicals, radioactive materials * comparable to domestic wastes 1. biodegradable 2. non biodegradable 3. recyclable

non-hazardous or general waste

# Categories of health care wastes * **Solid wastes** from patients with infectious disease (dressing, swabs, blood bags, urine bag, sputum cups) * **liquid wastes** from patients with infectious diseases (feces, urine, blood, or other body secretions) * **Cultures and stocks of infectious agents **from lab work

infectious wastes

# Categories of health care wastes * discarded needles used to give shots or draw blood * discarded lancets * scalpels, knives, blades, broken glass

sharps

# Categories of health care wastes * tissue sections and body material derived from biopsies or surgical procedures * placenta, internal organs, tissues used for diagnostic procedures such as biopsy, blood, fetus

pathological waste

# Categories of health care wastes * subgroup of pathological wastes * **recognizable body parts** usually from amputation procedures

anatomical wastes

# Categories of health care wastes * expired, spilt, contaminated pharmaceutical products, drug, and vaccines * antineoplastic, cytotoxic, genotoxic wastes (used in oncology or radiotherapy) * Biological fluids from patient treated with the said drugs

pharmaceutical waste

# Categories of health care wastes * hazardous and non-hazardous * Hazardous chemicals * With high content of heavy metals

chemical wastes

# Hazardous chemicals health and environment hazards

toxic

# Hazardous chemicals pH < 2.0, and bases > 12.0

corrosive

# Hazardous chemicals flash point below 60°C

flammable

# Hazardous chemicals explosive with water

reactive

# Categories of health care wastes * subcategory of hazardous chemical wastes * lead, mercury, cadmium

with high content of heavy metal

# Categories of health care wastes 3 heavy metals in hazardous chemical wastes

* lead * mercury * cadmium

# Categories of health care wastes * cylinders, cartridges, aerosol cans * containers may explode

pressurized containers

# Categories of health care wastes * radioactive materials * excreta of patients who underwent radionuclide diagnostic and therapeutic appications

radioactive wastes

# Color coding for waste segregation infectious

yellow

# Color coding for waste segregation pathological and anatomic

yellow

# Color coding for waste segregation sharps

red bin (no liner)

# Color coding for waste segregation pressurized containers

red

# Color coding for waste segregation chemical wastes

yellow with black band

# Color coding for waste segregation pharmaceutical waste

yellow with black band

# Color coding for waste segregation general waste - nonbiodegradable

black or colorless

# Color coding for waste segregation general waste - biodegrdable

green

# Treatment of health care waste prcess of changing the biological and chemical charactertistic of wastes to minimze its potential to cause harm

treatment

# Treatment of health care waste rely on heat to destroy pathogens

thermal processes

# Treatment of health care waste * **thermal decomposition** of health care wastes * absence of supplied molecular oxygen in the destruction chamber * waste is converted into gaseous, liquid, or solid form

pyrolysis

# Treatment of health care waste Where is the residues of pyrolysis disposed?

landfill

# Treatment of health care waste * use of **steam sterilization** * wet thermal * 121°C heat pressure of 15 psi * 15 - 30 mins

autoclave

# Treatment of health care waste 2 indicators of complete sterilization

* color-changing tapes * biologic test ampules containing bacterial spores

# Treatment of health care waste * 100°C for 30 mins * microorganisms are destroyed by **heat** which coagulates and denatures enzymes and proteins

microwave

# Treatment of health care waste at what temperature and for how many minutes is microwave done?

100°C at 30 minutes

# Treatment of health care waste * aldehydes, chlorine compounds, phenolic compounds * pathological waste, chemotherapy waste, chemical waste

chemical

# Treatment of health care waste * uses **enzyme** mixture to deconataminate health care wastes

biological process

# Treatment of health care waste breaks down the DNA molecules of the microorganisms

radiation technology

# Treatment of health care waste * filling up of containers with waste, adding immobilizing material, then sealing the container * disposed in landfill sites * pharmaceutical waste

encapsulation

# Treatment of health care waste * variant of encapsulation * pharmaceuticals ground then mix with water, cement, and lime to form a homogenous paste * disposed in landfill sites * pharmaceutical waste

inertization

# Disposal of health care waste * engineered site designed to keep waste isolated from the environment

sanitary landfill

# Disposal of health care waste * **treated** infectious wastes, sharps, pathological and anatomical wastes * health care facilities in remote areas

safe burial

# Disposal of health care waste what is disposed of in septic or concrete vaults?

sharps

# Type of waste, storage, collection on, treatment, collectionoff,disposal * kitchen waste * ____ * garbage holding area * ____

* green container * composting

# Type of waste, storage, collection on, treatment, collectionoff,disposal * kitchen waste * ____ * Garbage holding area * ____ * animal feeds

* green container * hog dealer & piggery owner

# Type of waste, storage, collection on, treatment, collectionoff,disposal * bottles & cans, ____ * ____ container * garbage holding area * hog dealer & piggery owner > ____ * junk shop dealer > ____

* paper & cartons * black * garbage holding area * animal feeds * recycling

# Type of waste, storage, collection on, treatment, collectionoff,disposal Infectious wastes

* infectious waste * yellow container * garbage holding area * non-burn technologies * city/municipal collection sysem * landfill

# Type of waste, storage, collection on, treatment, collectionoff,disposal * Pathological waste * ____ container > Cr____ * Refrigerator * Chemical disinfectant > ________ (2)

* yellow > crematorium * concrete vault, burying

# Type of waste, storage, collection on, treatment, collectionoff,disposal * Chemical waste * ____ container * (2 possible collection on site) * Waste disposal if non-hazardous liquid chemical? * Delay to decay > ____ technologies > ____ > recycling

* Yellow * Designated storage area, delay to decay * sink * non-burn technologies, city/municipal collection system

# Type of waste, storage, collection on, treatment, collectionoff,disposal Radioactive waste

* Radioactive waste * orange container * designated storage area * delay to decay * collection by supplier * disposal by supplier

# Type of waste, storage, collection on, treatment, collectionoff,disposal * Aerosol can * ____ contaner * designated storage area 1. Crushed > ____ > ____ 2. Collection by ____ > ____

* red 1. city/municipal collection system > landfill 2. collection by supplier > disposal by supplier

# Type of waste, storage, collection on, treatment, collectionoff,disposal * sharps * ____ * designated storage area 1. non-burn technologies > ____ > ____ 2. Chemical disinfectant > ____

* puncture proof containers 1. city/municipal collection system > landfill 2. burying

# Type of waste, storage, collection on, treatment, collectionoff,disposal pharmaceutical wastes

* pharmaceutical waste (expired) * yellow container * designated storage area * delay to decay * collection by supplier * disposal by supplier

# Suggested Guidelines for Spx Retention: Time duration Serum/CSF/body fluids

48 hours

# Suggested Guidelines for Spx Retention: Time duration urine

24 hours

# Suggested Guidelines for Spx Retention: Time duration Blood/fluid smears

7 days

# Suggested Guidelines for Spx Retention: Time duration microbilogy stained studies

7 days

# Suggested Guidelines for Spx Retention: Time duration Surgical pathology slides

10 years