Fungal Testing and Organisms 1 Flashcards

(15 cards)

1
Q

What are eukaryotic organisms?

A

contain a rigid wall composed of chitin and glucan. cell membrane contains ergosterol

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2
Q

What are the two major fungi groups?

A

yeasts and molds

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3
Q

describe yeast

A

most will grow on routine culture in 24-48 hours. will gram stain gram positive. they reproduce with budding.

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4
Q

describe molds

A

they produce hyphae: tube-like structures. reproduce asexually with spores. do not stain well with gram stain.

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5
Q

What is unique about a mold culture?

A

It requires special media. mold is held for 4-6 weeks.

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6
Q

Describe the specimen collection for hair.

A

scrap scalp with blunt scapula. the following specimens are also acceptable: hair stubs, plugged follicles, skin scales, hair plucked via forceps. CUT HAIR IS NOT ACCEPTABLE

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7
Q

Describe the specimen collection for skin

A

cleanse skin with approved antiseptic. scrap with scalpel and place in dry container transport at ambient room temperature.

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8
Q

What are acceptable nail samples?

A

scrapings, clippings, deep scrapings under nails

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9
Q

Ordering a fungal culture in blood requires…

A

a specific order. one culture taken per day. antiseptic techniques used.

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10
Q

How do you collect urine, sputum, and body fluids?

A

the same way as bacteria.

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11
Q

What are the 3 stains available to detect specimens?

A

gram = detects yeast, silver= detects yeast and fungal elements, calcoflour= fluorescent stain

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12
Q

What is a KOH prep?

A

KOH dissolves keratin material making fungal structures easier to see. it is a rapid test, often performed as a point of care test.

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13
Q

Can you use antigen detection to see if there is fungi?

A

yes. It usually gets rapid results: reported as positive eor neg. an example is cryptococcus neoformans.

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14
Q

When is nucleic acid testing usually performed?

A

for organisms that require long incubation periods or serious disease states.

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15
Q

When is there an interim report for a fungal culture?

A

after 48 hours, but it can take longer to grow. it may not be necessary to order a fungal culture for common fungal organisms.

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