Flashcards in Fungal Testing and Organisms 1 Deck (15):
1
What are eukaryotic organisms?
contain a rigid wall composed of chitin and glucan. cell membrane contains ergosterol
2
What are the two major fungi groups?
yeasts and molds
3
describe yeast
most will grow on routine culture in 24-48 hours. will gram stain gram positive. they reproduce with budding.
4
describe molds
they produce hyphae: tube-like structures. reproduce asexually with spores. do not stain well with gram stain.
5
What is unique about a mold culture?
It requires special media. mold is held for 4-6 weeks.
6
Describe the specimen collection for hair.
scrap scalp with blunt scapula. the following specimens are also acceptable: hair stubs, plugged follicles, skin scales, hair plucked via forceps. CUT HAIR IS NOT ACCEPTABLE
7
Describe the specimen collection for skin
cleanse skin with approved antiseptic. scrap with scalpel and place in dry container transport at ambient room temperature.
8
What are acceptable nail samples?
scrapings, clippings, deep scrapings under nails
9
Ordering a fungal culture in blood requires...
a specific order. one culture taken per day. antiseptic techniques used.
10
How do you collect urine, sputum, and body fluids?
the same way as bacteria.
11
What are the 3 stains available to detect specimens?
gram = detects yeast, silver= detects yeast and fungal elements, calcoflour= fluorescent stain
12
What is a KOH prep?
KOH dissolves keratin material making fungal structures easier to see. it is a rapid test, often performed as a point of care test.
13
Can you use antigen detection to see if there is fungi?
yes. It usually gets rapid results: reported as positive eor neg. an example is cryptococcus neoformans.
14
When is nucleic acid testing usually performed?
for organisms that require long incubation periods or serious disease states.
15