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Fungi Flashcards

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1
Q

What fungi is depicted?

A

Conidiophore (specifically this is Aspergillus)

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2
Q

What are the Superficial Mycoses Fungi?

A

Malassezia Furfur; Trichosporon

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3
Q

What is the classification of the fungi depicted? What structure is the arrow pointing to?

A

Ascomycetes, Ascus

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4
Q

What is the reproduction of: Zygomycetes, Ascomycetes, Basidiomycetes?

A

Sexual

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5
Q

What stain is being used here? What is the fungi shown?

A

PAS; Candida albicans

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6
Q

What occurs in sexual reproduction before a zygote is formed?

A

Schmoo Phase

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7
Q

What is the action of Triazole drug group?

A

Azoles inhibit the 14 alpha-demethylation of lanosterol to ergosterol without affecting Host cell membrane cholesterol synthesis.

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8
Q
A
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9
Q

What are examples of asexual reproduction?

A

Sporangium, Conidiophore, Arthorospores, yeast

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10
Q
A
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11
Q

What stain is being used? What is depicted? How do you know?

A

Gram Stain, Candida Albicans, Budding is occuring

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12
Q

What phylum/class is Cryptococcus, Malassezia and Trichosporon all apart of?

A

Basidiomycota; Basidiomycetes

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13
Q

How do you classify Histoplasma?

A

Pathogenic - Systemic

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14
Q

What is the identifying structures of Candida albicans?

A

Cells round to oval, pseudohyphae, and some true hyphae with clusters of round blastoconidia at the septa

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15
Q

What fungi is being depicted? What is the morphology of this fungi?

A

Rhizopus; Mold with coenocytic hyphae, spores in sporangium

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16
Q

What fungi is depicted? What structure is unique?

A

Cryptococcus; “Clubbing” basidiospores

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17
Q

How do you classify Aspergillus?

A

Pathogenic - Cutaneous; AND Opportunistic pathogens

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18
Q

What Drug is being used at E? What drug is used at C?

A

C: Azole (Triazole)

E: Amphotericin B

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19
Q

Why is it hard to use gram staining for fungi identification?

A

Everything is gram positive; so it can easily be confused with bacteria

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20
Q

What are examples of Ascomycota Saccharomycetes?

A

Candida, Saccharomyces

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21
Q

How do you classify Candida?

A

Pathogenic - Cutaneous; AND Opportunistic pathogens

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22
Q

What does Malassezia furfur require to grow on agar?

A

Requires Olive oil or some other source of long-chain fatty acids.

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23
Q

What drug inhibits the 14 alpha-demethylation of lanosterol to ergosterol without affecting Host cell membrane cholesterol synthesis?

A

Azole

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24
Q

What stain is being used here? What fungi is present?

A

H & E; Histoplasma Capsulatum

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25
What type of fungi is depicted?
Aspergillus
26
What is an example of Ascomycota Eurtiomycetes?
Aspergillus
27
28
What fungi is depicted?
This is the sporulation form Macroconidia (Microsporum Canis)
29
What fungi is depicted?
Microsporum Canis
30
What are the common histopathological stains for fungi?
H&E, GMS, PAS, Mucicarmine
31
What fungi is identified by: Cells round to oval, pseudohyphae, and some true hyphae with clusters of round blastoconidia at the septa
Candida Albicans
32
What is an example of Ascomycota Pneumocystidomycetes?
Pneumocystis jirovecii
33
What fungi is being depicted?
34
What structure is formed during Meiosis?
Zygospore
35
how do you classify Coccidioidies?
True Pathogen - System
36
What are examples of Basidiomycota Basidiomycetes fungi?
Cryptococcus, Malassezia, Trichosporon
37
What are examples of Sexual reproduction?
Zygomycetes, Ascomycetes, Basidiomycetes
38
What is the stain being used? What is the fungi depicted?
GMS Stain; Mucor
39
What is the action of Amphotericin B?
it is a Polyene (many double bonds) class of drug; it will bind to the Ergosterol in the fungus cell membrane to create artifical pores
40
What are the Opportunistic pathogens in fungi?
Aspergillus, Candida, Cryptococcus, Pneumocystis, Zygomycosis
41
Which drug creates pores in the Fungi cell membrane?
Amphotericin B
42
What is the stain being used? What is the fungi depicted?
GMS & PAS; Cryptococcus
43
What stain is being used? What is the fungi being depicted? What structure is being shown?
Lactophenol Cotton Blue; Rhizopus, showing Sporangiophores
44
What is an example of a Glomeromycota Zygomycetes fungi?
Rhizopus
45
What should be added to the media when fungi is expected?
Brain heart Infusion (BHI); Sabouraud dextrose & BHI (SABHI)
46
What stain is being used? What fungi is being depicted? What disease does it cause?
Lactophenol Cotton Blue; Malassezia fur fur - Cutaneous (dandruff; why it requires Olive Oil)
47
What kind of Fungi is depicted?
Trichosporon - Arranged into rectangular arthroconidia
48
What is the stain being used? What is the fungi depicted?
India Ink, Cryptococcus; Note the distinguishing Halo
49
What is the stain being used? What is the fungi depicted? What name was given to this famous culture?
H & E; Cryptococcus;
50
What type of fungi is Malassezia Furfur?
Pathogenic - superficial Mycoses
51
What phylum/class is Rhizopus?
Glomeromycota Zygomycetes
52
What are the three fundamental approaches for Identification of Fungi?
Microbiological; Histolopathological; Immunological
53
What phylum/class is Aspergillus?
Ascomycota Eurtiomycetes
54
What is the classification of the fungi here? What is the structure at the end of the arrow?
Zygomycetes; This is a zygospore
55
What is the stain being used? What is the fungi depicted?
Mucicarmine; Cryptococcus \*Note the distinguishing Halo
56
What are Candida and Saccharomyces phylum/class?
Ascomycota Saccharomycetes
57
What phylum/class is Pneumocystis jirovecii?
Ascomycota
58
What are the two true cutaneous pathogens?
Candida, Aspergillus
59
What is the reproduction of: Sporangium, Conidiophore, Arthorospores, yeast
Asexual
60
What are the common micrososcopy stains for Fungi?
Gram stain, Geimsa, Silver, Calcofluor, Lactophenol Cotton Blue

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