Gastrointestinal Cultures Flashcards

(63 cards)

1
Q

Differential media

A

Differentiation of bacterial species based on colony morphology

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2
Q

Selective media

A
  • limit growth of normal flora

- enhance growth of pathogens

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3
Q

What does Hektoen grow?

A

Only gram (-)

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4
Q

SMAC plate color

A

Pink

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5
Q

E.coli colony color on XLD

A

Yellow

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6
Q

Salmonella colony color on XLD

A

Black

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7
Q

TCBS plate color

A

Green

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8
Q

What color colonies does Vibrio produce?

A

Yellow

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9
Q

Most commonly ID’d intestinal parasite in the US; contaminated water (mountain streams)

A

Giardia lamblia

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10
Q

Troph form can migrate to liver and cause liver abscess

A

Entamoeba histolytica

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11
Q
  • resistant to chlorine
  • public swimming pool outbreaks
  • fecally contaminated food/water
  • international produce
  • p2p
A

Cryptosporidium parvum and C. hominid

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12
Q
  • contaminated food, water, and produce

- mostly soon in travelers returning from endemic areas

A

Cyclospora

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13
Q

Cause of diarrhea in immunocompromised individuals, particularly patients with AIDS

A

Microsporidia

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14
Q

Which pathogens have longer incubation periods and make patients sicker longer?

A

Parasitic pathogens

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15
Q

How do you diagnose parasitic pathogens?

A

Physically exam stool OR antigen detection / serological kits

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16
Q

Does not cause diarrhea but is associated with peptic ulcer disease

A

Heliobacter pylori

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17
Q
  • Food poisoning

- neurotoxin

A

Staphylococcus aureus / Bacillus cereus

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18
Q

Other bacterial pathogens

A
  • Plesiomonas
  • Aeromonas
  • Edwardsiella
  • Listeria monocytogenes
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19
Q

Viral GI pathogens

A
  • Rotavirus
  • Enteric adenovirus
  • Calcivirus
  • Astrovirus
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20
Q

What type of GI pathogen is rotavirus?

A

Viral

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21
Q

What type of GI pathogen is Enteric adenovirus?

A

Viral

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22
Q

What type of GI pathogen is Calcivirus?

A

Viral

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23
Q

What type of GI pathogen is Astrovirus?

A

Viral

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24
Q

Most common cause of diarrheal illness in children

A

Rotavirus

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25
Causes GI infections
Enteric adenovirus
26
Norwalk virus / cruise ships
Calcivirus
27
Infections in very old and young
Astrovirus
28
GI parasitic infection
- Giardia lamblia - Entamoeba histolytica - Cryptosporidium parvum and C. hominis - Cyclospora - Microsporidia
29
Giardia lamblia is a:
GI parasitic infection
30
Entamoeba histolytica is a:
GI parasitic infection
31
Cryptosporidium parvum and C. hominis are:
GI parasitic infections
32
Cyclospora is a:
GI parasitic infection
33
Microsporidia is a:
GI parasitic infection
34
Collection of GI specimen
- nonpreserved stool - fresh stool, qtip - spread thin layer on slide, wright stain - look for WBCs - WBCs indicate inflammatory diseases
35
GI host defenses | -colon
IgA (antibody) produced fight off some organisms
36
Bacterial Pathogens of the GI tract
- Campylobacter jejuni - Salmonella typhi - Shigella sonnei - Yersinia enterocolitica - Vibrio cholera - E.coli O157:H7 - Clostridium difficile - Staphylococcus aureus and Bacillus cereus - Heliobacter pylori - Asromonas and Plesiomonas
37
Causes the most severe form of enteric fever: typhoid fever
Salmonella typhi
38
Humans are the only known host for this pathogen
Salmonella typhi
39
Spread by fecally contaminated food or water and causes an inflammatory response
Salmonella typhi
40
Bacteria survive the host immune cells so it is considered an intracellular pathogen
Salmonella typhi
41
Normal GI flora are predominantly what species?
Anaerobic
42
GI host defenses | -what does normal flora do?
Fights off pathogens
43
GI host defenses | -stomach acidity
Most pathogens never reach the intestines because of the acidic environment of the stomach
44
GI host defense | -intestines - peristalsis
Constant motion that pushes substances through the intestines and reduces chances for adherence
45
Mucous layer
GI host pathogen
46
Antibiotic associated diarrhea caused by drug disrupting the normal indigenous flora of the colon
Clostridium difficile
47
Produces exotoxins A and B, easily spread by fecal/oral route, spore and toxin producing
Clostridium difficile
48
Infections more common in winter months
Yersinia enterocolitica
49
Linked to meat, unpasteurized milk and other dairy products
Yersinia enterocolitica
50
Refrigeration does not prevent growth of the organism
Yersinia enterocolitica
51
Causative agent of cholera
Vibrio cholera
52
Fish and shellfish
Vibrio cholera
53
Known to produce "rice-water" stools, risk of death from dehydration
Vibrio cholera
54
Specific strain that causes shiga-like toxins
E.coli O157:H7
55
Is E.coli a normal inhabitant of the GI tract?
Yes
56
Some patients develop chronic colonization leading to persistent shedding of the organism with potential transmission to others (Typhoid Mary)
Salmonella typhi
57
The most common species in the US, one of the most easily communicable bacterial diarrheal pathogens
Shigella sonnei
58
Produces several toxins including shiga toxin which can have cytotoxic, enterotoxic and neurotoxic effects
Shigella sonnei
59
Antibiotics can delay clinical improvement
Shigella sonnei
60
Most common bacterial cause of gastroenteritis in the world
Campylobacter jejuni
61
Contaminated poultry
Campylobacter jejuni
62
What is Guillain-Barré syndrome?
Life-threatening neurological disease
63
What causes Guillan-Barre syndrome and reactive arthritis?
Campylobacter jejuni