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MLT 140 > Enterobacteriaceae, Introduction & Testing > Flashcards

Enterobacteriaceae, Introduction & Testing Flashcards

(118 cards)

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1
Q

What do Methyl Red and Vogues-Proskaur determine?

A

End products of glucose fermentation

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2
Q

What does the Indole test determine?

A

If Indole is formed from tryptophan by tryptophanase

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3
Q

What does the urease test determine?

A

Hydrolysis of urea

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4
Q

What does Simmon’s citrate determine?

A

If citrate can be used as the sole carbon source

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5
Q

What does the Lysine Iron Agar (LIA) determine?

A

Lysine decarboxylase activity

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6
Q

What is SMAC?

A

MacConkey agar with soribitol

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7
Q

What does alpha hemolysis look like?

A

Green discoloration of the media

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8
Q

What does beta hemolysis look like?

A

Complete clearing of erythrocytes around colonies

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9
Q

What does Triple Sugar Iron (iron) agar determine?

A

Glucose and lactose, or sucrose utilization and hydrogen sulfide production

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10
Q

Which GI pathogen is characterized by watery diarrhea which turns to bloody diarrhea but has no leukocytes?

A

E.coli O157:H7

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11
Q

Media used for detection of salmonella and shigella

A

XLD, HEK, SS

One or the other

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12
Q

Media used for isolation of Yersinia only, incubates at room temperature

A

CIN

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13
Q

Media that selectively enhances the growth of salmonella and shigella while suppressing the growth of normal bowel flora, also used for the detection of shiga-like toxin producing strains of E.coli

A

GN broth/selenite

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14
Q

Media for isolation of Campylobacter - incubates at 42 Celsius

A

Campy agar

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15
Q

Media for isolation of Vibrio

A

TCBS

Thiosulfate-citrate-bile salts-sucrose

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16
Q

Media for detection of pathogenic strains of E.coli

A

MAC Sorbitol

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17
Q

Media for isolation of salmonella

A

Bismuth sulfite

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18
Q

Immunologic tests available for:

A

Shiga-toxins, campylobacter, C.diff, H.pylori

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19
Q

GI culture collection

-if not processed quickly, should be collected and transported in:

A

Cary-Blair, Amie’s or Stuart media

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20
Q

Microscopic examination of stool for:

A

WBCs - inflammatory diseases

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21
Q

GI culture collection

-preservatives should be used if examination for _________ is intended

A

Ova and parasites

-avoid preservatives if cultures are ordered

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22
Q

At the very least, stool cultures should set up media for the detection of:

A

Salmonella, shigella, campylobacter

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23
Q

_______ and _____ media are used to ID bacterial pathogens in stool

A

Selective and differential

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24
Q

_______ media contain antimicrobial said or chemicals to limit the growth of normal bacterial flora and to enhance the growth of pathogenic bacteria

A

Selective

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25
Salmonella TSI results
K/A+
26
E.coli TSI results
A/Ag
27
Proteus TSI results
K/A+
28
Klebsiella TSI results
A/Ag
29
Shigella TSI results
K/A
30
A pink colony on SMAC indicates what organism?
E.coli other than O157:H7
31
The Kaufman White Scheme is used to serotype which organism?
Salmonella
32
Name the organism that can lead to a carrier-state in the host after infection for which removal of the gallbladder is sometimes the only cure
Salmonella
33
Which of the Enterobacteriaceae is known to be highly drug resistant?
Klebsiella
34
Which GI pathogen requires only a very small inoculum and it also resists stomach acid?
Shigella
35
Which E.coli causes traveler's diarrhea?
ETEC
36
Which E.coli causes Hemolytic uremic syndrome (HUS)?
EHEC
37
Which E.coli causes dysentery?
EIEC
38
CIN
Yersinia
39
SMAC
E.coli except O157:H7
40
Swarming colonies
Proteus
41
Bismuth sulfite
Salmonella
42
Red pigment
Serratia
43
Burnt chocolate smell
Proteus
44
ESBL
Klebsiella
45
Vi antigen
Salmonella
46
Mug (+)
E.coli except O157:H7
47
Widely test (+)
Salmonella
48
Safety pin appearance on gram stain
Yersinia
49
How does a positive motility test appear?
Cloudiness around inoculation line
50
Name a test that detects motility
SIM
51
Name an organism that would give a positive motility reaction
E.coli
52
Name an organism that would give a negative motility reaction
Klebsiella
53
The production of acetylmethylcarbinol from dextrose is the principle of which test?
Vogues-Proskaur
54
In which test is ammonia formed?
Urease
55
What is the amino acid from which indole is formed in the indole test?
Tryptophan
56
What must be added to the indole test before reading?
Kovac's or Erlich's reagent
57
How would the following tests be resulted for klebsiella? Indole, citrate, VP
Indole: (-) Citrate: (+) VP: (+)
58
If the methyl red test is red, the MLT should report the test as:
Positive
59
If a SIM media is black, what does this indicate?
Positive - production of hydrogen sulfide
60
What is Christensen's media used for?
Citrate
61
What is the pH indicator in TSI?
Phenol red
62
Name a urea positive organism:
Proteus mirabilis
63
After the addition of Kovac's reagent, there is a pink color at the interface of the reagent and the media. This is the ______ test
Indole
64
In the TSI, the media is pushed up off the bottom of the tube. What would this indicate?
Gas production
65
If a TSI is solid yellow, how would you report it? What sugars were utilized?
A/A Glucose, lactose and or sucrose fermentation
66
If a citrate is green, it is reported as _____. Name an organism that would give this result
- negative | - E.coli
67
Name the three sugars in TSI
- glucose - lactose - sucrose
68
If a urea test is pink, how would it be reported?
Positive
69
Name an organism that would give a pink result on a urea test
Proteus mirabilis
70
What must be added to a VP test before the test can be interpreted?
Alpha naphthalene and KOH
71
Positive reaction appearance for VP
Red
72
Positive reaction appearance for Indole
Red
73
Positive reaction appearance for LIA
Purple
74
Which organism has an IMViC reaction | of ++--?
E.coli
75
How is klebsiella differentiated from Enterobacter biochemically?
Klebsiella is ornithine (-) and motile | Enterobacter is ornithine (+) and nonmotile
76
Which test produces acetylmethylcarbinol from dextrose?
Voges Proskauer
77
Which test causes fermentation of glucose?
Methyl red test
78
Which test produces ammonia?
Urea test
79
Which test uses Simmons medium?
Citrate test
80
Which test uses Christensen's medium?
Urea test
81
Which test utilizes citrate as a carbon source?
Citrate test
82
If an Enterobacteriaceae is motile, where on the organism are the flagella found?
All the way around | Peritrichous
83
A yellow color appears in a nitrite test after the addition of zinc dust. This result is considered to be:
Positive
84
A red result in LIA indicates
Deanimation of lysine
85
Which test is KOH associated with?
Voges Proskauer
86
Which test is Kovac associated with?
Indole
87
Which test is Alpha naphthal associated with?
Voges Proskauer
88
Which test is Erlich associated with?
Indole
89
Utilization of sucrose I'm TSI is demonstrated by the appearance of:
Yellow/yellow slant
90
A methyl red test result is yellow. This indicates a ________ reaction
Negative
91
A TSI that should be K/A is read too early. How will it appear?
A/A
92
Name the reagents that must be added to the Voges-Proskauer test before it can be interpreted
Alpha naphthol and KOH
93
Which E.coli causes infantile diarrhea?
EPEC
94
What causes typhoid?
Salmonella
95
What causes Pseudomembranous colitis?
Clostridium
96
What causes HUS?
E.coli O157:H7
97
What causes dysentery?
Shigella
98
How does shigella appear on MAC and HEK?
- Colorless on MAC | - green/clear on HEK
99
What is shigella's TSI?
K/A
100
Name three organisms that are phenylalanine deanimase
- proteus - providencia - morganella
101
Is salmonella normal flora in stool?
No, it's always a pathogen
102
If the MUG test is negative, what possible pathogen is indicated?
EHEC - Enterohemmoragic | E.coli O157:H7
103
How can shigella and salmonella be differentiated by TSI?
Shigella - K/A | Salmonella - K/A++
104
Name an organism that produces a dark red pigment
Serratia
105
What is the name of the chromogen that causes this red pigmentation?
Prodigiosin
106
The TSI is red over yellow. How would this be reported?
K/A
107
Enterobacteriaceae gram stain reaction:
Gram negative rods or coccobacilli
108
Enterobacteriaceae glucose reaction
Fermentation
109
Enterobacteriaceae oxidase reaction:
Negative
110
If a colony is pink on MAC, this indicates:
Lactose fermenter
111
An organism that has a green metallic sheen on EMB would be identified as:
E.coli
112
Positive citrate
Klebsiella
113
Positive Indole
E.coli | Proteus
114
Positive urease
Klebsiella | Proteus
115
Positive VP
Klebsiella
116
What causes cholera?
Vibrio
117
What causes plague?
Yersinia
118
What causes ulcers?
Heliobacter

MLT 140 (3 decks)

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