Flashcards in Gene Expression In Mammalian Cells Deck (5)
What are the most common cell types used in mammalian expression systems?
Epithelial and fibroblast
What are the 4 mammalian expression systems?
What are the advantages and disadvantages of a mammalian expression system?
+ proper modifications
+ several vectors available
+ well characterised
- low cell density
- slow expansion
- bovine proteins in culture media
What are 4 ways of studying gene expression in mammalian cells?
DNA microarrays - compare effects of different treatments
Protein microarrays - study expression at protein level
RNAi - silence expression of a particular gene
Gene over expression - study effects of over expression