Gene Therapy 2

Question 1

What are biomarkers in cancer?

Answer 1

Biomarkers are produced by cancer cells or by the body in response to cancer.

Question 2

Where can biomarkers be found?

Answer 2

Biomarkers can be found in blood, urine, stool, and biopsies.

Question 3

What are the uses of biomarkers in cancer?

Answer 3

Biomarkers are used for diagnosis, prognosis, and treatment monitoring.

Question 4

Define immunodetection

Answer 4

Term to describe the detection of a specific antigen using antigen binding. Interested in the antigen binding site at the end. Looking at antibodies that have been raised against a particular antigen. This technique allows us to determine whether a specific protein is in the sample or not

Question 5

What does ELISA stand for?

Answer 5

Enzyme-Linked Immunosorbent Assay

Question 6

What does ELISA identify?

Answer 6

Detects specific antigens/antibodies in a sample.

Question 7

What are the applications of this detection method?

Answer 7

Useful in research and clinically to detect infection or disease markers.

Question 8

What type of antibodies are used in ELISA?

Answer 8

Uses enzyme-linked secondary antibodies.

Question 9

What is involved in the process of detection?

Answer 9

Involves adsorption of antigen or antibody to multiwell plates.