General Characteristics & Others

Question 1

: Filaments of fungi

Answer 1

Hyphae

Question 2

: Mats of hyphae

Answer 2

Mycelia

Question 3

: viruses w bacterial host

Answer 3

Bacteriophage

Question 4

: causes transmissible spongiform encephalopathy

Answer 4

Prions

Question 5

Spirilla is motile by:
Spirochete is motile by:

Answer 5

Flagella

Axial Filaments

Question 6

Cellular arrangement forming “Picket Fence” / “Chinese Character” appearance

Answer 6

Palisades

Question 7

Test to identify encapsulated organisms

Answer 7

Neufeld Quellung Test

Question 8

Teichoic acid in cell wall is a characteristic of gram ____

Answer 8

Positive

Question 9

Beta-lactam antibiotics resistance:
G (+):
G(-):

Answer 9

Sensitive
Resistant

Question 10

End product of Fluorochrome stains:
Acid-fast:
Non-acid fast:

Answer 10

Yellow fluorescence
Red fluorescence

Question 11

Method which uses 0.5-1% sulfuric acid decolorizer

Answer 11

Modified Kinyoun Method

Question 12

Organisms considered as mollicutes

Answer 12

Mycoplasma and ureaplasma

Question 13

: temporary loss of cell wall

Answer 13

L-form

Question 14

: site of energy metabolism

Answer 14

Plasma membrane

Question 15

Point of attachment of chromosome

Answer 15

Mesosome

Question 16

Metachromatic granules
- Babes Ernst Granules:
- Much granules:
- Bipolar bodies (Safety pin appearance):

Answer 16

: Corynebacterium diptheria
: Mycobacterium tuberculosis
: Yersinia pestis, Pasteurella

Question 17

: flagella-like organelles in periplasmic space (not true flagella)

Answer 17

Endoflagella

Question 18

: allows bacteria to resist heat and chemicals; found in the endospore

Answer 18

Calcium dipicolinate

Question 19

: temperature best for locomotion

Answer 19

25deg C (Mr)

Question 20

CHARACTERISTIC MOTILITY
: Listeria
: Campylobacter
: Capnocytophaga
: Proteus, C. Tetani, C. Septicum

Answer 20

: Tumbling
: darting
: gliding
: swarming

Question 21

Biosafety cabinet used for handling radioisotype carcinogens

Answer 21

Biosafety cabinet level II, B1 / B2

Question 22

HEPA means

Answer 22

High efficiency particulate air

Question 23

Minimum volume of specimen for body fluids sample

Answer 23

1mL

10-15mL for Fungal/Mycobacteria samples

Question 24

CSF sample order of collection:

Answer 24

1. Chemistry
2. Microbiology
3. Hematology

CoMe Here

Question 25

1 bottle CSF specimen, order of sections for testing:

Answer 25

Microbio——Hema——Chem

Question 26

Preferred urine sample for anaerobic culture:

Answer 26

Suprapubic aspiration

Question 27

Stool to preservative ratio

Answer 27

1:3

Question 28

: method used for examination of motility

Answer 28

Hanging drop method *True Motility - characteristic motility* *Brownian Motility - random movement*

Question 29

Liquid media (+) growth result/reaction

Answer 29

Increase in turbidity

Question 30

: used to get rid of bubbles in culture media preparation

Answer 30

Pass the flame of a bunsen burner quickly over the surface of the agar

Question 31

: to prevent formation of water droplets

Answer 31

Store upside down

Question 32

: technique used for determining hemolysis pattern

Answer 32

Transillumination technique *light source from bottom of plate*

Question 33

: alpha hemolysis surrounded by beta hemolysis

Answer 33

Alpha-prime / wide zone *Viridans group of Streptococcus*

Question 34

: Double zone of hemolysis

Answer 34

Target hemolysis *Bull’s-eye* *C.perfringens*

Question 35

: lowest amount of antibiotic that prevents visible growth

Answer 35

Minimal Inhibitory Concentration (MIC)

Question 36

: concentration of antibiotic resulting in 99.9% reduction of CFU/mL

Answer 36

Minimal Bactericidal Concentration

Question 37

Standard-sized inoculum- compare broth to __________

Answer 37

0.5 McFarland Standard *bacterial suspension of 1.5x10^8 CFU/mL*

Question 38

Zone of inhibition and MIC relationship

Answer 38

Inversely proportional *INC zone of inhibition = DEC MIC*

Question 39

: used to induce resistance to clindamycin in D-test

Answer 39

Erythromycin

Question 40

: uses a plastic strip w/ antibiotic concentration gradient

Answer 40

E-test / Epsilometer test

Question 41

: supplements added to MHA in Oxacillin screen plate

Answer 41

4% NaCl and 6ug/mL Oxacillin

Question 42

Test to detect Methicillin-resistant S.aureus

Answer 42

Oxacillin screen plate

Question 43

Aerobic g(+) bacilli

Answer 43

**STARiNG (branching)** Streptomyces Tsukamurella Actinomadura Rhodococcus Nocardia Gordonia **LAGE BC (non-branching)** Listeria Arcanobacterium Gardnerella vaginallis Erysipelothrix Bacillus (spore former)