genetic engineering

Question 1

genetic engineering

Answer 1

manip of an org’s DNA

Question 2

tranformed organisms

Answer 2

DNA altered by GE

Question 3

recombinant DNA

Answer 3

mol of DNA consists of DNA from two sources joined togther

Question 4

final goal of techniques

Answer 4

getting a host org to prod proteins encoded by a new gene that has been inserted into the host’s DNA

Question 5

why are plasmids a perfect vector

Answer 5

can be transcribed by having simple bacterial promoter region upstream of the gene

Question 6

main req of org

Answer 6

large quantites of a protien can be prod as cheaply and easily as possible

Question 7

org needs to be

Answer 7

grow faast
easily manip
naturally occing vectors- plasmids

Question 8

good option for org

Answer 8

yeasts or bacteria

Question 9

b4 insersion of gene

Answer 9

same r,endonucleases used to cut plasmid

Question 10

where do r.endo cut plasmid

Answer 10

specific recognion sites

Question 11

what does r.endo create in plasmid

Answer 11

complimentary sticky ends

Question 12

what forms bw bases

Answer 12

h bonds

Question 13

DNA ligase

Answer 13

joins DNA togther by caalysing formation of phosphodiester bonds

Question 14

waht are ppd bonds formed bw

Answer 14

sugar and phosphate groups of the backbone

Question 15

transformation

Answer 15

injecting the vector in2 the chosen bacterium

Question 16

why are vectors contaning genes moved to living cells

Answer 16

replication and/ expression

Question 17

host cell

Answer 17

cells recieving the vector

Question 18

vectors

Answer 18

lasrge mols which dont readily cross cell membranes

Question 19

how are vectors made permeable

Answer 19

electroporation
viruses
microinjection
liposomes

Question 20

electroporation

Answer 20

cells subjected to high voltage pulse, temporarily distrupts the mem and allows vectpr to enter cell

Question 21

viruses

Answer 21

vector incrorp into a virus

inj host cels by inj gen mat- containing selected gene

Question 22

microinjection

Answer 22

gen mat inj using a micro pipette

Question 23

Ti plasmids

Answer 23

only plasmids plant cells take up

Question 24

liposomes

Answer 24

DNA warpped in a lipid mol allowing it to pass through phospholipid bilayer

Question 25

steps of gene cloning

Answer 25

isolate gene
produce recombinant plasmid
tranfrom bacteria that will express the gene

Question 26

isolating euk gene to be cloned

Answer 26

extrac mrna
reverse transcriptase
prod cdna- no introns
pcr to amplify cdna

Question 27

identifyin dna transformed w/recomb plasmids

Answer 27

marker genes- abiotic res/flourescent genes

Question 28

Recognition sites of sticky eNTs are

Answer 28

Palindromic- read same way backwards