GMO Lab Part II Flashcards Preview

BIOC 3622 - Biochemistry Lab > GMO Lab Part II > Flashcards

Flashcards in GMO Lab Part II Deck (12)
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1
Q

Immediately after PCR amplification, can you determine whether or not you have PCR products?

A

No.

2
Q

To visualize your PCR products, what method/tool will you use?

A

Gel electrophoresis

3
Q

Your PCR product bands are very ____ compared to those in other DNA experiments you may have done.

A

small

4
Q

What is needed to impede the movement of these bands so that it is separated more on the gel and can be seen?

A

Tighter gel matrix

5
Q

How much agarose is needed to use agarose electrophoresis?

A

3%

6
Q

What is the alternative gel instead of agarose gel?

A

polyacrylamide gel

7
Q

What is used to separate smaller molecules for visualization?

A

PAGE: polyacrylamide gel electrophoresis

8
Q

What reference will you use to determine the product bands’ sizes?

A

molecular weight ruler (DNA standard)

9
Q

After the molecular weight ruler is loaded, what stain will be used to stain the gel?

A

Fast Blast stain

10
Q

Why will Fast Blast stain be used to stain the gel?

A

To make the bands visible.

11
Q

What is the absorbance of PMM?

A

455 bp

12
Q

What is the absorbance of GMO?

A

200 bp