Golgi Flashcards
(30 cards)
What is the role of ER Exit Sites in vesicular transport?
They form transport vesicles and properly package them for transport to the Golgi
What gives vesicles their unique ‘fuzzy’ surface appearance?
COP attached to the outside surface of the vesicle membrane.
Name the three classes of vesicle coat proteins.
COPI, COPII and Clathrin
In what direction does COPI travel?
Backwards from the Golgi to the ER, and backwards within the Golgi as well
Vesicles that transport proteins from the Golgi and PM to endosomes are called;
Clathrin-coated vesicles
Explain the function of Sar1-GDP.
It changes into Sar1-GTP to integrate into the ERES to create the bud that becomes a vesicle.
True or False: ERES-derived vesicles traffic to the trans Golgi network.
False. They go to the cis Golgi network, which is closest to the ER.
What mediates the movement of vesicles through the cytosol?
Cytoskeleton highways, and molecular motors that move them across the highways.
What is the function of Rab proteins?
They recognize incoming vesicle and recipient membranes
What is the name of the protein that forms a ‘bridge’ between vesicles and recipient membranes?
Tethering proteins
The docking of vesicles is mediated by which proteins?
SNAREs
True or False: SNAREs are located on every transport vesicle and recipient membrane.
True. They are necessary for proper docking
What is the difference between t-SNAREs and v-SNAREs?
v-SNAREs and found on vesicles, and t-SNAREs are found on acceptor membranes.
What type of sequence is responsible for escaped protein ER retrieval?
C-terminal KDEL sequence
True or False: COPI recognizes the KKxx sequence on escaped ER membrane proteins.
True. This is how it is transported back to the ER from the Golgi.
True or False: plant and animal cells have one large Golgi complex.
False. Plant cells often contain numerous Golgi complexes.
How are the different Golgi sub compartments held together?
With GRASP tethering proteins
What are Golgins?
Long, filamentous proteins that tether the Golgi to the cytoskeleton
What is the name of the protein that facilitates ER to Golgi communication through microtubules?
GMAPs
What is the function of the cis Golgi Network?
To receive transport vesicles from the ER and sort materials for transport.
Name the three main sections of the Golgi cisternae.
The cis, medial and trans cisternae
Which part of the Golgi mediates Golgi metabolism?
The cisternae
What two types of protein coatings will transport vesicles that come from the trans Golgi network have?
Clathrin if the vesicle is going to the endosome or PM, or COPI if it’s going back to the cisternae
Which organelle is known as the ‘processing plant’ of the cell?
The Golgi Complex
